The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis.
Open accessAbstract
Centromere protein-F (CENP-F) is mammalian kinetochore protein that was recently identified by an autoimmune serum (Rattner, J. B., A. Rao, M. J. Fritzler, D. W. Valencia, and T. J. Yen. Cell Motil. Cytoskeleton. 26:214-226). We report here the human cDNA sequence of CENP-F, along with its expression and localization patterns at different stages of the HeLa cell cycle. CENP-F is protein of the nuclear matrix that gradually accumulates during the cell cycle until it reaches peak levels in G2 and M phase cells and is rapidly degraded upon completion of mitosis. CENP-F is first detected at the prekinetochore complex during late G2, and is clearly detectable as paired foci that correspond to all the centromeres by prophase. During mitosis, CENP-F is associated with kinetochores from prometaphase until early anaphase and is then detected at the spindle midzone throughout the remainder of anaphase. By telophase, CENP-F is concentrated within the intracellular bridge at either side of the mid-body. The predicted structure of the 367-kD CENP-F protein consists of two 1,600-amino acid-long coil domains that flank a central flexible core. A putative P-loop nucleotide binding site (ADIPTGKT) is located within the globular carboxy terminus. The structural features deduced from our sequence studies and the spatial and temperal distribution of CENP-F revealed in our cytological and biochemical studies suggest that it may play a role in several mitotic events.
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Metadata
- Authors
- Liao, H., Winkfein, R. J., Mack, G., Rattner, J. B., & Yen, T. J.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1995-08-01
- Volume
- 130
- Issue
- 3
- Pages
- 507-518
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2100147739
- Cited by
- 384
- References
- 34
- FWCI
- 5.38
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis. https://app.cochat.ai/papers/10.1083/jcb.130.3.507
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- Liao, H., Winkfein, R. J., Mack, G., Rattner, J. B., & Yen, T. J. (1995). CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis. The Journal of cell biology. https://doi.org/10.1083/jcb.130.3.507
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