Mannose 6-Phosphate Receptors Regulate the Formation of Clathrin-coated Vesicles in the TGN
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The transport of the two mannose 6-phosphate receptors (MPRs) from the secretory pathway to the endocytic pathway is mediated by carrier vesicles coated with the AP-1 Golgi-specific assembly protein and clathrin. Using an in vitro assay that reconstitutes the ARF-1–dependent translocation of cytosolic AP-1 onto membranes of the TGN, we have previously reported that the MPRs are key components for the efficient recruitment of AP-1 (Le Borgne, R., G. Griffiths, and B. Hoflack. 1996. J. Biol. Chem. 271:2162–2170). Using a polyclonal antibody against the mouse γ-adaptin, we have now examined the steady state distribution of AP-1 after subcellular fractionation of mouse fibroblasts lacking both MPRs or reexpressing physiological levels of either MPR. We report that the amount of AP-1 bound to membranes and associated with clathrin-coated vesicles depends on the expression level of the MPRs and on the integrity of their cytoplasmic domains. Thus, these results indicate that the concentration of the MPRs, i.e., the major transmembrane proteins sorted toward the endosomes, determines the number of clathrin-coated vesicles formed in the TGN.
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Recruitment of AP-1 to TGN membranes is regulated by Arf1 and Arf4 small G proteins.
“Given that the recruitment of AP-1 to TGN membranes has been reported to be regulated by both Arf1 (Stamnes and Rothman, 1993; Le Borgne and Hoflack, 1997; Lee et al., 2008) and Arf4 (Lowery et al., 2013; Nakai et al., 2013) small G proteins”
From Distinct anterograde trafficking pathways of BACE1 and amyloid precursor protein from the TGN and the regulation of amyloid-β production (2020) · cites this paper as Le Borgne and Hoflack, 1997
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Structural relationships between clathrin assembly proteins from the Golgi and the plasma membrane.
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Tan, J. Z. A., Fourriere, L., Wang, J., Perez, F., Boncompain, G., & Gleeson, P. A. (2020) · Molecular Biology of the Cell · doi:10.1091/mbc.e19-09-0487 · Cited by 37 · see citing works
- Authors
- Borgne, R. L., & Hoflack, B.
- Affiliations
- European Molecular Biology Laboratory (DE)
- Corresponding author
- Hoflack, Bernard
- ORCID
- Borgne, Roland Le
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1997-04-21
- Volume
- 137
- Issue
- 2
- Pages
- 335-345
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC2139777
- OpenAlex
- W2156516862
- Cited by
- 112
- References
- 1
- Citations by year
- 2026: 2 · 2025: 1 · 2023: 3 · 2021: 2 · 2020: 2 · 2019: 1 · 2018: 1 · 2017: 4 · 2016: 2 · 2015: 1 · 2014: 2 · 2012: 3
- FWCI
- 4.67
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Cell Biology (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Keywords
- mannose 6-phosphate receptor, clathrin-coated vesicles, trans-Golgi network, AP-1 complex, vesicle formation, endocytic pathway, secretory pathway, receptor cytoplasmic domain, fibroblasts
- MeSH terms
- Amino Acid Sequence, Animals, Biological Transport, Cell Fractionation, Cells, Cultured, Clathrin, Cytoplasm, Endocytosis, Fibroblasts, Golgi Apparatus, Intracellular Membranes, Membrane Proteins, Molecular Sequence Data, Mutation, Receptors, Transferrin, Biomarkers, Receptor, IGF Type 2, Coated Vesicles, Adaptor Proteins, Vesicular Transport, Adaptor Protein Complex alpha Subunits, Adaptor Protein Complex gamma Subunits, Mice
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