The DOI matches its Crossref record; 55 of the 55 references it cites were found in a registry or an index.
Spindle Assembly in Xenopus Egg Extracts: Respective Roles of Centrosomes and Microtubule Self-Organization
Open accessAbstract
In Xenopus egg extracts, spindles assembled around sperm nuclei contain a centrosome at each pole, while those assembled around chromatin beads do not. Poles can also form in the absence of chromatin, after addition of a microtubule stabilizing agent to extracts. Using this system, we have asked (a) how are spindle poles formed, and (b) how does the nucleation and organization of microtubules by centrosomes influence spindle assembly? We have found that poles are morphologically similar regardless of their origin. In all cases, microtubule organization into poles requires minus end–directed translocation of microtubules by cytoplasmic dynein, which tethers centrosomes to spindle poles. However, in the absence of pole formation, microtubules are still sorted into an antiparallel array around mitotic chromatin. Therefore, other activities in addition to dynein must contribute to the polarized orientation of microtubules in spindles. When centrosomes are present, they provide dominant sites for pole formation. Thus, in Xenopus egg extracts, centrosomes are not necessarily required for spindle assembly but can regulate the organization of microtubules into a bipolar array.
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Cites
Beyond self-assembly: From microtubules to morphogenesis
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A Complex of NuMA and Cytoplasmic Dynein Is Essential for Mitotic Spindle Assembly
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Cytoplasmic dynein is localized to kinetochores during mitosis
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Cited by
Meraldi, P. (2002) · The EMBO Journal · doi:10.1093/emboj/21.4.483 · Cited by 658
Ran Induces Spindle Assembly by Reversing the Inhibitory Effect of Importin α on TPX2 Activity
Gruss, O. J., Carazo-Salas, R. E., Schatz, C. A., Guarguaglini, G., Kast, J., Wilm, M., Le Bot, N., Vernos, I., Karsenti, E., & Mattaj, I. W. (2001) · Cell · doi:10.1016/s0092-8674(01)00193-3 · Cited by 593
Self-citationAssembly of centrosomal proteins and microtubule organization depends on PCM-1
Dammermann, A., & Merdes, A. (2002) · The Journal of Cell Biology · doi:10.1083/jcb.200204023 · Cited by 489
Centrosome duplication in mammalian somatic cells requires E2F and Cdk2–Cyclin A
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CENP-E is a plus end-directed kinetochore motor required for metaphase chromosome alignment
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Metadata
- Authors
- Heald, R., Tournebize, R., Habermann, A., Karsenti, E., & Hyman, A.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1997-08-11
- Volume
- 138
- Issue
- 3
- Pages
- 615-628
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2042901082
- Cited by
- 342
- References
- 55
- FWCI
- 9.79
- Type
- journal article
- Language
- en
- Open access
- green
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- Heald, R., Tournebize, R., Habermann, A., Karsenti, E., & Hyman, A. (1997). Spindle Assembly in Xenopus Egg Extracts: Respective Roles of Centrosomes and Microtubule Self-Organization. The Journal of Cell Biology. https://doi.org/10.1083/jcb.138.3.615
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