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Cited by 348Cell Adhesion Molecules ResearchBarrier Structure and Function StudiesWnt/β-catenin signaling in development and cancer

α-Catenin-Vinculin Interaction Functions to Organize the Apical Junctional Complex in Epithelial Cells

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Watabe-Uchida, Mitsuko, Uchida, Naoshige, Imamura, Yuzo, Nagafuchi, Akira, Fujimoto, Kazushi, Uemura, Tadashi, Vermeulen, Stefan, van Roy, Frans +2 more·1998·The Journal of Cell Biology
DOI 10.1083/jcb.142.3.847PMC PMC2148175
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Abstract

αE-catenin, a cadherin-associated protein, is required for tight junction (TJ) organization, but its role is poorly understood. We transfected an αE-catenin–deficient colon carcinoma line with a series of αE-catenin mutant constructs. The results showed that the amino acid 326–509 domain of this catenin was required to organize TJs, and its COOH-terminal domain was not essential for this process. The 326–509 internal domain was found to bind vinculin. When an NH2-terminal αE-catenin fragment, which is by itself unable to organize the TJ, was fused with the vinculin tail, this chimeric molecule could induce TJ assembly in the αE-catenin–deficient cells. In vinculin-null F9 cells, their apical junctional organization was impaired, and this phenotype was rescued by reexpression of vinculin. These results indicate that the αE-catenin-vinculin interaction plays a role in the assembly of the apical junctional complex in epithelia.

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  • In simple epithelia, vinculin was shown to control apical positioning of the ZA.

    “In simple epithelia, vinculin was shown to control apical positioning of the ZA43.”

    From E-cadherin integrates mechanotransduction and EGFR signaling to control junctional tissue polarization and tight junction positioning (2017) · cites this paper as [43]

    The Results section, “Apical Junctional Organization in Vinculin-deficient Cells” (page unavailable), shows that loss of vinculin disrupted apical ZO-1 networks in F9 cells and that restoring vinculin rescued them. The Discussion proposes a role for vinculin in extending the ZA along apicolateral surfaces, but does not show that vinculin controls the ZA’s apical position in simple epithelia; it also cautions that F9 cells may not represent mature epithelial cells. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

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Authors
Watabe-Uchida, M., Uchida, N., Imamura, Y., Nagafuchi, A., Fujimoto, K., Uemura, T., Vermeulen, S., van Roy, F., Adamson, E. D., & Takeichi, M.
Affiliations
  • *Department of Biophysics, Faculty of Science, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502; ‡Department of Cell Biology and §Department of Anatomy, Faculty of Medicine, Kyoto University, Yoshida, Sakyo-ku, Kyoto 606-8507, Japan; ‖University Hospital of Ghent and ¶Department of Molecular Biology, VIB-University of Ghent, B-9000 Ghent, Belgium; and **The Burnham Institute, La Jolla, California
  • Sanford Burnham Prebys Medical Discovery Institute (US)
ORCID
Venue
The Journal of Cell Biology
Publisher
Rockefeller University Press
Published
1998-08-10
Volume
142
Issue
3
Pages
847-857
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
DOI
10.1083/jcb.142.3.847
PMC
PMC2148175
OpenAlex
W2069990200
Cited by
348
References
1
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FWCI
8.30
Type
journal article
Language
en
Open access
Locations
  • The Journal of Cell Biology (publishedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
  • Ghent University Academic Bibliography (Ghent University) (submittedVersion, other-oa)
Funders
Japan Society for the Promotion of Science
Topics
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
Keywords
alphaE-catenin, vinculin, tight junction assembly, apical junctional complex, epithelial cells, F9 cells, colon cancer cells, protein-protein interaction
MeSH terms
Binding Sites, Cell Communication, Cytoskeletal Proteins, Epithelial Cells, Humans, Intercellular Junctions, Membrane Proteins, Phosphoproteins, Recombinant Fusion Proteins, Tumor Cells, Cultured, Vinculin, alpha Catenin, Zonula Occludens-1 Protein

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