The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Correlative Light-Electron Microscopy Reveals the Tubular-Saccular Ultrastructure of Carriers Operating between Golgi Apparatus and Plasma Membrane
Open accessAbstract
Transport intermediates (TIs) have a central role in intracellular traffic, and much effort has been directed towards defining their molecular organization. Unfortunately, major uncertainties remain regarding their true structure in living cells. To address this question, we have developed an approach based on the combination of the green fluorescent protein technology and correlative light-electron microscopy, by which it is possible to monitor an individual carrier in vivo and then take a picture of its ultrastructure at any moment of its lifecycle. We have applied this technique to define the structure of TIs operating from the Golgi apparatus to the plasma membrane, whose in vivo dynamics have been characterized recently by light microscopy. We find that these carriers are large (ranging from 0.3–1.7 μm in maximum diameter, nearly half the size of a Golgi cisterna), comprise almost exclusively tubular-saccular structures, and fuse directly with the plasma membrane, sometimes minutes after docking to the fusion site.
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Cites
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Self-citationKirchhausen, T. (2000) · Nature Reviews Molecular Cell Biology · doi:10.1038/35043117 · Cited by 560
Correlated light and electron microscopy: ultrastructure lights up!
de Boer, P., Hoogenboom, J. P., & Giepmans, B. N. G. (2015) · Nature Methods · doi:10.1038/nmeth.3400 · Cited by 526
Maintenance of Golgi structure and function depends on the integrity of ER export
Ward, T. H., Polishchuk, R. S., Caplan, S., Hirschberg, K., & Lippincott-Schwartz, J. (2001) · The Journal of Cell Biology · doi:10.1083/jcb.200107045 · Cited by 420
Self-citationModulation of Membrane Curvature by Phosphatidic Acid and Lysophosphatidic Acid
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Metadata
- Authors
- Polishchuk, R. S., Polishchuk, E. V., Marra, P., Alberti, S., Buccione, R., Luini, A., & Mironov, A. A.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2000-01-10
- Volume
- 148
- Issue
- 1
- Pages
- 45-58
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2051463269
- Cited by
- 340
- References
- 34
- FWCI
- 6.77
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Correlative Light-Electron Microscopy Reveals the Tubular-Saccular Ultrastructure of Carriers Operating between Golgi Apparatus and Plasma Membrane. https://app.cochat.ai/papers/10.1083/jcb.148.1.45
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- Polishchuk, R. S., Polishchuk, E. V., Marra, P., Alberti, S., Buccione, R., Luini, A., & Mironov, A. A. (2000). Correlative Light-Electron Microscopy Reveals the Tubular-Saccular Ultrastructure of Carriers Operating between Golgi Apparatus and Plasma Membrane. The Journal of Cell Biology. https://doi.org/10.1083/jcb.148.1.45
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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