The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
Identification and Characterization of Sa/Scc3p Subunits in the Xenopus and Human Cohesin Complexes
Open accessAbstract
A multisubunit protein complex, termed cohesin, plays an essential role in sister chromatid cohesion in yeast and in Xenopus laevis cell-free extracts. We report here that two distinct cohesin complexes exist in Xenopus egg extracts. A 14S complex (x-cohesinSA1) contains XSMC1, XSMC3, XRAD21, and a newly identified subunit, XSA1. In a second 12.5S complex (x-cohesinSA2), XSMC1, XSMC3, and XRAD21 associate with a different subunit, XSA2. Both XSA1 and XSA2 belong to the SA family of mammalian proteins and exhibit similarity to Scc3p, a recently identified component of yeast cohesin. In Xenopus egg extracts, x-cohesinSA1 is predominant, whereas x-cohesinSA2 constitutes only a very minor population. Human cells have a similar pair of cohesin complexes, but the SA2-type is the dominant form in somatic tissue culture cells. Immunolocalization experiments suggest that chromatin association of cohesinSA1 and cohesinSA2 may be differentially regulated. Dissociation of x-cohesinSA1 from chromatin correlates with phosphorylation of XSA1 in the cell-free extracts. Purified cdc2-cyclin B can phosphorylate XSA1 in vitro and reduce the ability of x-cohesinSA1 to bind to DNA or chromatin. These results shed light on the mechanism by which sister chromatid cohesion is partially dissolved in early mitosis, far before the onset of anaphase, in vertebrate cells.
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Citations
Cites
Genetic instabilities in human cancers
Lengauer, C., Kinzler, K. W., & Vogelstein, B. (1998) · Nature · doi:10.1038/25292 · Cited by 4,168 · cited 2×
“Malfunction in any one of these processes would result in chromosome missegregation and aneuploidy, potentially contributing to birth defects or tumor development (for reviews see Hassold and Jacobs, 1984; Lengauer et al., 1998).”
BackgroundCyclin is degraded by the ubiquitin pathway
Glotzer, M., Murray, A. W., & Kirschner, M. W. (1991) · Nature · doi:10.1038/349132a0 · Cited by 2,357
Cohesins: Chromosomal Proteins that Prevent Premature Separation of Sister Chromatids
Michaelis, C., Ciosk, R., & Nasmyth, K. (1997) · Cell · doi:10.1016/s0092-8674(01)80007-6 · Cited by 1,471
Sister-chromatid separation at anaphase onset is promoted by cleavage of the cohesin subunit Scc1
Uhlmann, F., Lottspeich, F., & Nasmyth, K. (1999) · Nature · doi:10.1038/21831 · Cited by 943
Guacci, V., Koshland, D., & Strunnikov, A. (1997) · Cell · doi:10.1016/s0092-8674(01)80008-8 · Cited by 828
Zou, H., McGarry, T. J., Bernal, T., & Kirschner, M. W. (1999) · Science · doi:10.1126/science.285.5426.418 · Cited by 805
Cohesin’s binding to chromosomes depends on a separate complex consisting of Scc2 and Scc4 proteins
Ciosk, R., Shirayama, M., Shevchenko, A., Tanaka, T., Toth, A., Shevchenko, A., & Nasmyth, K. (2000) · Molecular Cell · doi:10.1016/s1097-2765(00)80420-7 · Cited by 755
Klein, F., Mahr, P., Galova, M., Buonomo, S. B. C., Michaelis, C., Nairz, K., & Nasmyth, K. (1999) · Cell · doi:10.1016/s0092-8674(00)80609-1 · Cited by 730
Hassold, T. J., & Jacobs, P. A. (1984) · Annual Review of Genetics · doi:10.1146/annurev.ge.18.120184.000441 · Cited by 651
Identification of Xenopus SMC protein complexes required for sister chromatid cohesion
Losada, A., Hirano, M., & Hirano, T. (1998) · Genes & Development · doi:10.1101/gad.12.13.1986 · Cited by 601
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Cited by
Nasmyth, K. (2001) · Annual Review of Genetics · doi:10.1146/annurev.genet.35.102401.091334 · Cited by 805
Wapl controls the dynamic association of cohesin with chromatin
Kueng, S., Hegemann, B., Peters, B. H., Lipp, J. J., Schleiffer, A., Mechtler, K., & Peters, J.-M. (2006) · Cell · doi:10.1016/j.cell.2006.09.040 · Cited by 624
The cohesin complex and its roles in chromosome biology
Peters, J.-M., Tedeschi, A., & Schmitz, J. (2008) · Genes & Development · doi:10.1101/gad.1724308 · Cited by 532
Gandhi, R., Gillespie, P. J., & Hirano, T. (2006) · Current Biology · doi:10.1016/j.cub.2006.10.061 · Cited by 441
Self-citationHauf, S., Roitinger, E., Koch, B., Dittrich, C. M., Mechtler, K., & Peters, J.-M. (2005) · PLoS Biology · doi:10.1371/journal.pbio.0030069 · Cited by 440
Metadata
- Authors
- Losada, A., Yokochi, T., Kobayashi, R., & Hirano, T.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2000-08-07
- Volume
- 150
- Issue
- 3
- Pages
- 405-416
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2170016329
- Cited by
- 317
- References
- 40
- FWCI
- 8.37
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Losada, A., Yokochi, T., Kobayashi, R., & Hirano, T. (2000). Identification and Characterization of Sa/Scc3p Subunits in the Xenopus and Human Cohesin Complexes. The Journal of Cell Biology. https://doi.org/10.1083/jcb.150.3.405
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