The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Cd44 Is a Major E-Selectin Ligand on Human Hematopoietic Progenitor Cells
Open accessAbstract
E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM). Though it is known that a glycoform of PSGL-1 (CLA) functions as the principal E-selectin ligand on human T lymphocytes, the E-selectin ligand(s) of human HPCs has not been identified. We used a shear-based adherence assay to analyze and define the E-selectin ligand activity of membrane proteins from human HPCs. Our data show that PSGL-1 expressed on human HPCs is an E-selectin ligand, and that HPCs also express a previously unrecognized E-selectin ligand, CD44. The E-selectin ligand activity of CD44 is conferred by the elaboration of sialylated, fucosylated binding determinants on N-glycans. This glycoform of CD44 is expressed on primitive CD34+ human HPCs, but not on more mature hematopoietic cells. Under physiologic flow conditions, this molecule mediates E-selectin–dependent rolling interactions over a wider shear range than that of PSGL-1, and promotes human HPC rolling interactions on E-selectin expressed on human BM endothelial cells. These findings offer new insights into the structural biology and physiology of CD44, and into the molecular basis of E-selectin–dependent adhesive interactions that direct homing of human HPC to BM.
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Cites
CD44 is the principal cell surface receptor for hyaluronate
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Selectins and their ligands: Current concepts and controversies
Kansas, G. (1996) · Blood · doi:10.1182/blood.v88.9.3259.bloodjournal8893259 · Cited by 1,415
ELAM-1 is an adhesion molecule for skin-homing T cells
Picker, L. J., Kishimoto, T. K., Smith, C. W., Warnock, R. A., & Butcher, E. C. (1991) · Nature · doi:10.1038/349796a0 · Cited by 826
Cutaneous lymphocyte antigen is a specialized form of PSGL-1 expressed on skin-homing T cells
Fuhlbrigge, R. C., Kieffer, J. D., Armerding, D., & Kupper, T. S. (1997) · Nature · doi:10.1038/40166 · Cited by 577
Self-citationStamenkovic, I., Aruffo, A. A., Amiot, M., & Seed, B. (1991) · The EMBO Journal · doi:10.1002/j.1460-2075.1991.tb07955.x · Cited by 573
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Cited by
Varki, A. (2017) · Glycobiology · doi:10.1093/glycob/cww086 · Cited by 2,635
“In the case of a CD44 glycoform from hematopoietic stem cells that is a specific ligand for E-and L-selectin (1077) (1078) , the authors reasonably chose to rename the molecule altogether as HCELL (hematopoietic cell E-and L-selectin ligand) (921) .”
BackgroundBlood on the tracks: hematopoietic stem cell-endothelial cell interactions in homing and engraftment
Perlin, J. R., Sporrij, A., & Zon, L. I. (2017) · Journal of Molecular Medicine · doi:10.1007/s00109-017-1559-8 · Cited by 52
Dendritic cell entry to lymphatic capillaries is orchestrated by CD44 and the hyaluronan glycocalyx
Johnson, L. A., Banerji, S., Lagerholm, B. C., & Jackson, D. G. (2021) · Life Science Alliance · doi:10.26508/lsa.202000908 · Cited by 37
Metadata
- Authors
- Dimitroff, C. J., Lee, J. Y., Rafii, S., Fuhlbrigge, R. C., & Sackstein, R.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2001-06-11
- Volume
- 153
- Issue
- 6
- Pages
- 1277-1286
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2006608487
- Cited by
- 317
- References
- 35
- FWCI
- 4.77
- Type
- journal article
- Language
- en
- Open access
- green
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- Dimitroff, C. J., Lee, J. Y., Rafii, S., Fuhlbrigge, R. C., & Sackstein, R. (2001). Cd44 Is a Major E-Selectin Ligand on Human Hematopoietic Progenitor Cells. The Journal of Cell Biology. https://doi.org/10.1083/jcb.153.6.1277
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