The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Spatio-temporal activation of caspase revealed by indicator that is insensitive to environmental effects
Open accessAbstract
Indicator molecules for caspase-3 activation have been reported that use fluorescence resonance energy transfer (FRET) between an enhanced cyan fluorescent protein (the donor) and enhanced yellow fluorescent protein (EYFP; the acceptor). Because EYFP is highly sensitive to proton (H+) and chloride ion (Cl−) levels, which can change during apoptosis, this indicator's ability to trace the precise dynamics of caspase activation is limited, especially in vivo. Here, we generated an H+- and Cl−-insensitive indicator for caspase activation, SCAT, in which EYFP was replaced with Venus, and monitored the spatio-temporal activation of caspases in living cells. Caspase-3 activation was initiated first in the cytosol and then in the nucleus, and rapidly reached maximum activation in 10 min or less. Furthermore, the nuclear activation of caspase-3 preceded the nuclear apoptotic morphological changes. In contrast, the completion of caspase-9 activation took much longer and its activation was attenuated in the nucleus. However, the time between the initiation of caspase-9 activation and the morphological changes was quite similar to that seen for caspase-3, indicating the activation of both caspases occurred essentially simultaneously during the initiation of apoptosis.
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Nagai, T., Ibata, K., Park, E. S., Kubota, M., Mikoshiba, K., & Miyawaki, A. (2002) · Nature Biotechnology · doi:10.1038/nbt0102-87 · Cited by 2,753
Self-citationA Combinatorial Approach Defines Specificities of Members of the Caspase Family and Granzyme B
Thornberry, N. A., Rano, T. A., Peterson, E. P., Rasper, D. M., Timkey, T., Garcia-Calvo, M., Houtzager, V. M., Nordstrom, P. A., Roy, S., Vaillancourt, J. P., et al. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.29.17907 · Cited by 2,068
Decreased apoptosis in the brain and premature lethality in CPP32-deficient mice
Kuida, K., Zheng, T. S., Na, S., Kuan, C.-Y., Yang, D., Karasuyama, H., Rakic, P., & Flavell, R. A. (1996) · Nature · doi:10.1038/384368a0 · Cited by 1,882
Mechanisms of Caspase Activation and Inhibition during Apoptosis
Shi, Y. (2002) · Molecular Cell · doi:10.1016/s1097-2765(02)00482-3 · Cited by 1,833
Reduced Apoptosis and Cytochrome c–Mediated Caspase Activation in Mice Lacking Caspase 9
Kuida, K., Haydar, T. F., Kuan, C.-Y., Gu, Y., Taya, C., Karasuyama, H., Su, M. S.-S., Rakic, P., & Flavell, R. A. (1998) · Cell · doi:10.1016/s0092-8674(00)81476-2 · Cited by 1,643
Differential Requirement for Caspase 9 in Apoptotic Pathways In Vivo
Hakem, R., Hakem, A., Duncan, G. S., Henderson, J. T., Woo, M., Soengas, M. S., Elia, A., de la Pompa, J. L., Kagi, D., Khoo, W., et al. (1998) · Cell · doi:10.1016/s0092-8674(00)81477-4 · Cited by 1,292
Cryns, V., & Yuan, J. (1998) · Genes & Development · doi:10.1101/gad.12.11.1551 · Cited by 1,228
Apaf1 Is Required for Mitochondrial Pathways of Apoptosis and Brain Development
Yoshida, H., Kong, Y.-Y., Yoshida, R., Elia, A. J., Hakem, A., Hakem, R., Penninger, J. M., & Mak, T. W. (1998) · Cell · doi:10.1016/s0092-8674(00)81733-x · Cited by 1,138
Llopis, J., McCaffery, J. M., Miyawaki, A., Farquhar, M. G., & Tsien, R. Y. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.12.6803 · Cited by 1,130
Self-citationApaf1 (CED-4 Homolog) Regulates Programmed Cell Death in Mammalian Development
Cecconi, F., Alvarez-Bolado, G., Meyer, B. I., Roth, K. A., & Gruss, P. (1998) · Cell · doi:10.1016/s0092-8674(00)81732-8 · Cited by 896
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Chudakov, D. M., Matz, M. V., Lukyanov, S., & Lukyanov, K. A. (2010) · Physiological Reviews · doi:10.1152/physrev.00038.2009 · Cited by 1,375
Evolutionary optimization of fluorescent proteins for intracellular FRET
Nguyen, A. W., & Daugherty, P. S. (2005) · Nature Biotechnology · doi:10.1038/nbt1066 · Cited by 678
Quantitative Analysis of Pathways Controlling Extrinsic Apoptosis in Single Cells
Albeck, J. G., Burke, J. M., Aldridge, B. B., Zhang, M., Lauffenburger, D. A., & Sorger, P. K. (2008) · Molecular Cell · doi:10.1016/j.molcel.2008.02.012 · Cited by 379
Tang, H. L., Tang, H. M., Mak, K. H., Hu, S., Wang, S. S., Wong, K. M., Wong, C. S. T., Wu, H. Y., Law, H. T., Liu, K., et al. (2012) · Molecular Biology of the Cell · doi:10.1091/mbc.e11-11-0926 · Cited by 293
Apico-basal forces exerted by apoptotic cells drive epithelium folding
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Metadata
- Authors
- Takemoto, K., Nagai, T., Miyawaki, A., & Miura, M.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2003-01-13
- Volume
- 160
- Issue
- 2
- Pages
- 235-243
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2007688166
- Cited by
- 271
- References
- 34
- FWCI
- 3.54
- Type
- journal article
- Language
- en
- Open access
- green
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