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Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis
Open accessAbstract
We have investigated the function of mitotic kinesin-like protein (MKlp) 2, a kinesin localized to the central spindle, and demonstrate that its depletion results in a failure of cleavage furrow ingression and cytokinesis, and disrupts localization of polo-like kinase 1 (Plk1). MKlp2 is a target for Plk1, and phosphorylated MKlp2 binds to the polo box domain of Plk1. Plk1 also binds directly to microtubules and targets to the central spindle via its polo box domain, and this interaction controls the activity of Plk1 toward MKlp2. An antibody to the neck region of MKlp2 that prevents phosphorylation of MKlp2 by Plk1 causes a cytokinesis defect when introduced into cells. We propose that phosphorylation of MKlp2 by Plk1 is necessary for the spatial restriction of Plk1 to the central spindle during anaphase and telophase, and the complex of these two proteins is required for cytokinesis.
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Cites
Self- and actin-templated assembly of Mammalian septins
Kinoshita, M., Field, C. M., Coughlin, M. L., Straight, A. F., & Mitchison, T. J. (2002) · Developmental Cell · doi:10.1016/s1534-5807(02)00366-0 · Cited by 526
A GRASP55-rab2 effector complex linking Golgi structure to membrane traffic
Short, B., Preisinger, C., Körner, R., Kopajtich, R., Byron, O., & Barr, F. A. (2001) · The Journal of Cell Biology · doi:10.1083/jcb.200108079 · Cited by 226
Self-citation
Cited by
Shared and separate functions of polo-like kinases and aurora kinases in cancer
Lens, S. M. A., Voest, E. E., & Medema, R. H. (2010) · Nature Reviews Cancer · doi:10.1038/nrc2964 · Cited by 597
Traffic control: regulation of kinesin motors
Verhey, K. J., & Hammond, J. W. (2009) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2782 · Cited by 546
Polo on the Rise—from Mitotic Entry to Cytokinesis with Plk1
Petronczki, M., Lénárt, P., & Peters, J.-M. (2008) · Developmental Cell · doi:10.1016/j.devcel.2008.04.014 · Cited by 485
Essential roles of KIF4 and its binding partner PRC1 in organized central spindle midzone formation
Kurasawa, Y., Earnshaw, W. C., Mochizuki, Y., Dohmae, N., & Todokoro, K. (2004) · The EMBO Journal · doi:10.1038/sj.emboj.7600347 · Cited by 333
Proteomic screen defines the Polo‐box domain interactome and identifies Rock2 as a Plk1 substrate
Lowery, D. M., Clauser, K. R., Hjerrild, M., Lim, D., Alexander, J., Kishi, K., Ong, S., Gammeltoft, S., Carr, S. A., & Yaffe, M. B. (2007) · The EMBO Journal · doi:10.1038/sj.emboj.7601683 · Cited by 257
Metadata
- Authors
- Neef, R., Preisinger, C., Sutcliffe, J., Kopajtich, R., Nigg, E. A., Mayer, T. U., & Barr, F. A.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2003-08-25
- Volume
- 162
- Issue
- 5
- Pages
- 863-876
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2069643337
- Cited by
- 319
- References
- 2
- FWCI
- 8.93
- Type
- journal article
- Language
- en
- Open access
- green
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- Neef, R., Preisinger, C., Sutcliffe, J., Kopajtich, R., Nigg, E. A., Mayer, T. U., & Barr, F. A. (2003). Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis. The Journal of Cell Biology. https://doi.org/10.1083/jcb.200306009
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