Actin dynamics coupled to clathrin-coated vesicle formation at the trans-Golgi network
Open accessAbstract
In diverse species, actin assembly facilitates clathrin-coated vesicle (CCV) formation during endocytosis. This role might be an adaptation specific to the unique environment at the cell cortex, or it might be fundamental, facilitating CCV formation on different membranes. Proteins of the Sla2p/Hip1R family bind to actin and clathrin at endocytic sites in yeast and mammals. We hypothesized that Hip1R might also coordinate actin assembly with clathrin budding at the trans-Golgi network (TGN). Using deconvolution and time-lapse microscopy, we showed that Hip1R is present on CCVs emerging from the TGN. These vesicles contain the mannose 6-phosphate receptor involved in targeting proteins to the lysosome, and the actin nucleating Arp2/3 complex. Silencing of Hip1R expression by RNAi resulted in disruption of Golgi organization and accumulation of F-actin structures associated with CCVs on the TGN. Hip1R silencing and actin poisons slowed cathepsin D exit from the TGN. These studies establish roles for Hip1R and actin in CCV budding from the TGN for lysosome biogenesis.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Arf activation promotes recruitment of several actin-regulatory proteins, including cortactin and dynamin, to the vesicle-budding sites of the trans-Golgi network (TGN).
“Recent studies have shown that Arf activation promotes recruitment of several actin-regulatory proteins, including cortactin and dynamin, to the vesicle-budding sites of the trans-Golgi network (TGN) [62], [63].”
From Arf4 Determines Dentate Gyrus-Mediated Pattern Separation by Regulating Dendritic Spine Development (2012) · cites this paper as [63]
The Introduction (page not available in our copy) says that clathrin-coated vesicle formation at the TGN involves recruitment of dynamin. The Results and discussion place the actin-regulatory Arp2/3 complex on TGN-derived vesicles. Neither passage supports the more specific claim that Arf activation recruits cortactin and dynamin to those sites. (LLM, conf=0.84)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
No references could be resolved yet.
Research works printed but not linked: 24
- Barbero, P., L. Bittova, and S.R. Pfeffer. 2002. Visualization of Rab9-mediated vesicle transport from endosomes to the trans-Golgi in living cells. J. Cell Biol. 156: 511–518. 11827983 PMC2173336
- Brodsky, F.M., C.Y. Chen, C. Knuehl, M.C. Towler, and D.E. Wakeham. 2001. Biological basket weaving: formation and function of clathrin-coated vesicles. Annu. Rev. Cell Dev. Biol. 17: 517–568. 11687498
- Campbell, C.H., and L.H. Rome. 1983. Coated vesicles from rat liver and calf brain contain lysosomal enzymes bound to mannose 6-phosphate receptors. J. Biol. Chem. 258: 13347–13352. 6138357
- Engqvist-Goldstein, A.E., and D.G. Drubin. 2003. Actin assembly and endocytosis: from yeast to mammals. Annu. Rev. Cell Dev. Biol. 19: 287–332. 14570572
- Engqvist-Goldstein, A.E., M.M. Kessels, V.S. Chopra, M.R. Hayden, and D.G. Drubin. 1999. An actin-binding protein of the Sla2/Huntingtin interacting protein 1 family is a novel component of clathrin-coated pits and vesicles. J. Cell Biol. 147: 1503–1518. 10613908 PMC2174256
- Engqvist-Goldstein, A.E., R.A. Warren, M.M. Kessels, J.H. Keen, J. Heuser, and D.G. Drubin. 2001. The actin-binding protein Hip1R associates with clathrin during early stages of endocytosis and promotes clathrin assembly in vitro. J. Cell Biol. 154: 1209–1223. 11564758 PMC2150824
- Engqvist-Goldstein, A.E., C.X. Zhang, S. Carreno, C. Barroso, J. Heuser, and D.G. Drubin. 2004. RNAi-mediated Hip1R silencing results in stable association between the endocytic machinery and the actin assembly machinery. Mol. Biol. Cell. 15: 1666–1679. 14742709 PMC379265
- Frischknecht, F., S. Cudmore, V. Moreau, I. Reckmann, S. Rottger, and M. Way. 1999. Tyrosine phosphorylation is required for actin-based motility of vaccinia but not Listeria or Shigella. Curr. Biol. 9: 89–92. 10021367
- Gaidarov, I., F. Santini, R.A. Warren, and J.H. Keen. 1999. Spatial control of coated-pit dynamics in living cells. Nat. Cell Biol. 1: 1–7. 10559856
- Godbold, G.D., K. Ahn, S. Yeyeodu, L.F. Lee, J.P. Ting, and A.H. Erickson. 1998. Biosynthesis and intracellular targeting of the lysosomal aspartic proteinase cathepsin D. Adv. Exp. Med. Biol. 436: 153–162. 9561213
- Jones, S.M., K.E. Howell, J.R. Henley, H. Cao, and M.A. McNiven. 1998. Role of dynamin in the formation of transport vesicles from the trans-Golgi network. Science. 279: 573–577. 9438853
- Kaksonen, M., Y. Sun, and D.G. Drubin. 2003. A pathway for association of receptors, adaptors, and actin during endocytic internalization. Cell. 115: 475–487. 14622601
- Llopis, J., J.M. McCaffery, A. Miyawaki, M.G. Farquhar, and R.Y. Tsien. 1998. Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins. Proc. Natl. Acad. Sci. USA. 95: 6803–6808. 9618493 PMC22642
- McDonald, K. 1984. Osmium ferricyanide fixation improves microfilament preservation and membrane visualization in a variety of animal cell types. J. Ultrastruct. Res. 86: 107–118. 6539826
- Merrifield, C.J., M.E. Feldman, L. Wan, and W. Almers. 2002. Imaging actin and dynamin recruitment during invagination of single clathrin-coated pits. Nat. Cell Biol. 4: 691–698. 12198492
- Motley, A., N.A. Bright, M.N. Seaman, and M.S. Robinson. 2003. Clathrin-mediated endocytosis in AP-2–depleted cells. J. Cell Biol. 162: 909–918. 12952941 PMC2172830
- Munier-Lehmann, H., F. Mauxion, U. Bauer, P. Lobel, and B. Hoflack. 1996. Re-expression of the mannose 6-phosphate receptors in receptor-deficient fibroblasts. Complementary function of the two mannose 6-phosphate receptors in lysosomal enzyme targeting. J. Biol. Chem. 271: 15166–15174. 8662879
- Okano, A., N. Usuda, K. Furihata, K. Nakayama, Q. Bao Tian, T. Okamoto, and T. Suzuki. 2003. Huntingtin-interacting protein-1-related protein of rat (rHIP1R) is localized in the postsynaptic regions. Brain Res. 967: 210–225. 12650982
- Percival, J.M., J.A. Hughes, D.L. Brown, G. Schevzov, K. Heimann, B. Vrhovski, N. Bryce, J.L. Stow, and P.W. Gunning. 2004. Targeting of a tropomyosin isoform to short microfilaments associated with the Golgi complex. Mol. Biol. Cell. 15: 268–290. 14528022 PMC307546
- Puertollano, R., R.C. Aguilar, I. Gorshkova, R.J. Crouch, and J.S. Bonifacino. 2001. Sorting of mannose 6-phosphate receptors mediated by the GGAs. Science. 292: 1712–1716. 11387475
- Rozelle, A.L., L.M. Machesky, M. Yamamoto, M.H. Driessens, R.H. Insall, M.G. Roth, K. Luby-Phelps, G. Marriott, A. Hall, and H.L. Yin. 2000. Phosphatidylinositol 4,5-bisphosphate induces actin-based movement of raft-enriched vesicles through WASP-Arp2/3. Curr. Biol. 10: 311–320. 10744973
- Schulze-Lohoff, E., A. Hasilik, and K. von Figura. 1985. Cathepsin D precursors in clathrin-coated organelles from human fibroblasts. J. Cell Biol. 101: 824–829. 3928634 PMC2113708
- Stinchcombe, J.C., L.J. Page, and G.M. Griffiths. 2000. Secretory lysosome biogenesis in cytotoxic T lymphocytes from normal and Chediak Higashi syndrome patients. Traffic. 1: 435–444. 11208129
- Welch, M.D., A.H. DePace, S. Verma, A. Iwamatsu, and T.J. Mitchison. 1997. The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly. J. Cell Biol. 138: 375–384. 9230079 PMC2138188
Cited by
Sorted by citations
del Toro, D., Canals, J. M., Ginés, S., Kojima, M., Egea, G., & Alberch, J. (2006) · The Journal of Neuroscience · doi:10.1523/jneurosci.3873-06.2006 · Cited by 95 · see citing works
Arf4 Determines Dentate Gyrus-Mediated Pattern Separation by Regulating Dendritic Spine Development
Jain, S., Yoon, S. Y., Zhu, L., Brodbeck, J., Dai, J., Walker, D., & Huang, Y. (2012) · PLoS ONE · doi:10.1371/journal.pone.0046340 · Cited by 43 · see citing works
- Authors
- Carreno, S., Engqvist-Goldstein, A. E., Zhang, C. X., McDonald, K. L., & Drubin, D. G.
- Affiliations
- 1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720
- University of California, Berkeley (US)
- ORCID
- Drubin, David G.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2004-06-21
- Volume
- 165
- Issue
- 6
- Pages
- 781-788
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC2172402
- OpenAlex
- W1985586898
- Cited by
- 118
- References
- 0
- Citations by year
- 2026: 4 · 2025: 1 · 2022: 3 · 2021: 4 · 2020: 1 · 2019: 2 · 2018: 1 · 2017: 3 · 2016: 2 · 2015: 3 · 2014: 6 · 2013: 6 · 2012: 6
- FWCI
- 4.72
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- The Journal of Cell Biology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (R01GM065462, GM65462)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Keywords
- clathrin-coated vesicles, actin dynamics, HIP1R, trans-Golgi network, endocytosis, Arp2/3 complex, mannose 6-phosphate receptor, lysosomal biogenesis, cathepsin D, Golgi organization, actin nucleation, F-actin accumulation
- MeSH terms
- Actins, Cathepsin D, HeLa Cells, Humans, Image Processing, Computer-Assisted, Lysosomes, Protein Binding, Proto-Oncogene Proteins pp60(c-src), Fluorescent Antibody Technique, Indirect, trans-Golgi Network, Clathrin-Coated Vesicles, RNA, Small Interfering, Adaptor Proteins, Signal Transducing
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
