Live imaging of wound inflammation in Drosophila embryos reveals key roles for small GTPases during in vivo cell migration
Open accessAbstract
Aa robust inflammatory response to tissue damage and infection is conserved across almost all animal phyla. Neutrophils and macrophages, or their equivalents, are drawn to the wound site where they engulf cell and matrix debris and release signals that direct components of the repair process. This orchestrated cell migration is clinically important, and yet, to date, leukocyte chemotaxis has largely been studied in vitro. Here, we describe a genetically tractable in vivo wound model of inflammation in the Drosophila melanogaster embryo that is amenable to cinemicroscopy. For the first time, we are able to examine the roles of Rho-family small GTPases during inflammation in vivo and show that Rac-mediated lamellae are essential for hemocyte motility and Rho signaling is necessary for cells to retract from sites of matrix– and cell–cell contacts. Cdc42 is necessary for maintaining cellular polarity and yet, despite in vitro evidence, is dispensable for sensing and crawling toward wound cues.
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In Drosophila embryos, Rho family small GTPases regulate hemocyte cytoskeleton during the rapid wound inflammatory response.
“In the Drosophila embryo, movies of hemocytes (the fly equivalent of macrophages) that are mutant for, or expressing dominant negative versions of, each of the Rho family small GTPases (guanosine triphosphatases) have revealed roles for these regulators of the cytoskeleton as hemocytes undergo the rapid wound inflammatory response (119).”
From Wound repair and regeneration: Mechanisms, signaling, and translation (2014) · cites this paper as [119]
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References 25 · Cited by 285
- Authors
- Stramer, B., Wood, W., Galko, M. J., Redd, M. J., Jacinto, A., Parkhurst, S. M., & Martin, P.
- Affiliations
- University of Bristol (GB)
- Instituto Gulbenkian de Ciência (PT)
- Howard Hughes Medical Institute (US)
- University College London (GB)
- 3Centro Biologia Desenvolvimento, Instituto Gulbenkian de Ciência, 2780-156 Oeiras, Portugal
- Fred Hutch Cancer Center (US)
- Corresponding author
- Martin, Paul
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2005-02-07
- Volume
- 168
- Issue
- 4
- Pages
- 567-573
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC2171743
- OpenAlex
- W2134090916
- Cited by
- 285
- References
- 25
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 8 · 2023: 7 · 2022: 6 · 2021: 11 · 2020: 16 · 2019: 9 · 2018: 11 · 2017: 12 · 2016: 11 · 2015: 18 · 2014: 24 · 2013: 23 · 2012: 17
- FWCI
- 5.79
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Cell Biology (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Bristol Research (University of Bristol) (submittedVersion)
- RVC Research Online (Royal Veterinary College) (submittedVersion, cc-by)
- Funders
- National Institute of General Medical Sciences (GM066847, R01GM066847)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Invertebrate Immune Response Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Keywords
- wound inflammation, Drosophila embryo, Rho GTPases, Rac, Rho, Cdc42, leukocyte chemotaxis, hemocyte migration, lamellae, cell polarity, in vivo imaging, tissue repair
- MeSH terms
- Animals, Chemotaxis, Leukocyte, Drosophila melanogaster, Embryo, Nonmammalian, Hemocytes, Inflammation, Intercellular Junctions, Lasers, Pseudopodia, Wound Healing, Signal Transduction, Cell Polarity, rho GTP-Binding Proteins, cdc42 GTP-Binding Protein
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