The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Molecular mechanisms of invadopodium formation
Open accessAbstract
Invadopodia are actin-rich membrane protrusions with a matrix degradation activity formed by invasive cancer cells. We have studied the molecular mechanisms of invadopodium formation in metastatic carcinoma cells. Epidermal growth factor (EGF) receptor kinase inhibitors blocked invadopodium formation in the presence of serum, and EGF stimulation of serum-starved cells induced invadopodium formation. RNA interference and dominant-negative mutant expression analyses revealed that neural WASP (N-WASP), Arp2/3 complex, and their upstream regulators, Nck1, Cdc42, and WIP, are necessary for invadopodium formation. Time-lapse analysis revealed that invadopodia are formed de novo at the cell periphery and their lifetime varies from minutes to several hours. Invadopodia with short lifetimes are motile, whereas long-lived invadopodia tend to be stationary. Interestingly, suppression of cofilin expression by RNA interference inhibited the formation of long-lived invadopodia, resulting in formation of only short-lived invadopodia with less matrix degradation activity. These results indicate that EGF receptor signaling regulates invadopodium formation through the N-WASP–Arp2/3 pathway and cofilin is necessary for the stabilization and maturation of invadopodia.
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Cites
Regulation of actin dynamics by WASP and WAVE family proteins
Stradal, T. E. B., Rottner, K., Disanza, A., Confalonieri, S., Innocenti, M., & Scita, G. (2004) · Trends in Cell Biology · doi:10.1016/j.tcb.2004.04.007 · Cited by 286
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The WASP-WAVE protein network: Connecting the membrane to the cytoskeleton.
Takenawa, T., & Suetsugu, S. (2007) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2069 · Cited by 947
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Lawson, C. D., & Ridley, A. J. (2018) · Journal of Cell Biology · doi:10.1083/jcb.201612069 · Cited by 539
Directed cell invasion and migration during metastasis
Bravo-Cordero, J. J., Hodgson, L., & Condeelis, J. (2012) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2011.12.004 · Cited by 462
Self-citationDual roles and therapeutic targeting of tumor-associated macrophages in tumor microenvironments
Xu, J., Ding, L., Mei, J., Hu, Y., Kong, X., Dai, S., Bu, T., Xiao, Q., & Ding, K. (2025) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-025-02325-5 · Cited by 389
Ins and outs of ADF/cofilin activity and regulation
Van Troys, M., Huyck, L., Leyman, S., Dhaese, S., Vandekerkhove, J., & Ampe, C. (2008) · European Journal of Cell Biology · doi:10.1016/j.ejcb.2008.04.001 · Cited by 329
Metadata
- Authors
- Yamaguchi, H., Lorenz, M., Kempiak, S., Sarmiento, C., Coniglio, S., Symons, M., Segall, J., Eddy, R., Miki, H., Takenawa, T., & Condeelis, J.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2005-01-31
- Volume
- 168
- Issue
- 3
- Pages
- 441-452
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2117116688
- Cited by
- 669
- References
- 1
- FWCI
- 14.59
- Type
- journal article
- Language
- en
- Open access
- green
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- Yamaguchi, H., Lorenz, M., Kempiak, S., Sarmiento, C., Coniglio, S., Symons, M., Segall, J., Eddy, R., Miki, H., Takenawa, T., & Condeelis, J. (2005). Molecular mechanisms of invadopodium formation. The Journal of Cell Biology. https://doi.org/10.1083/jcb.200407076
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