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Cited by 169Cellular transport and secretionMonoclonal and Polyclonal Antibodies ResearchDrug Transport and Resistance Mechanisms

The recycling and transcytotic pathways for IgG transport by FcRn are distinct and display an inherent polarity

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Tzaban, Salit, Massol, Ramiro H., Yen, Elizabeth, Hamman, Wendy, Frank, Scott R., Lapierre, Lynne A., Hansen, Steen H., Goldenring, James R. +2 more·2009·Journal of Cell Biology
DOI 10.1083/jcb.200809122PMC PMC2711563
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Abstract

The Fc receptor FcRn traffics immunoglobulin G (IgG) in both directions across polarized epithelial cells that line mucosal surfaces, contributing to host defense. We show that FcRn traffics IgG from either apical or basolateral membranes into the recycling endosome (RE), after which the actin motor myosin Vb and the GTPase Rab25 regulate a sorting step that specifies transcytosis without affecting recycling. Another regulatory component of the RE, Rab11a, is dispensable for transcytosis, but regulates recycling to the basolateral membrane only. None of these proteins affect FcRn trafficking away from lysosomes. Thus, FcRn transcytotic and recycling sorting steps are distinct. These results are consistent with a single structurally and functionally heterogeneous RE compartment that traffics FcRn to both cell surfaces while discriminating between recycling and transcytosis pathways polarized in their direction of transport.

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Authors
Tzaban, S., Massol, R. H., Yen, E., Hamman, W., Frank, S. R., Lapierre, L. A., Hansen, S. H., Goldenring, J. R., Blumberg, R. S., & Lencer, W. I.
Affiliations
  • Brigham and Women's Hospital (US)
  • Vanderbilt University (US)
Corresponding author
Lencer, Wayne I.
ORCID
Venue
Journal of Cell Biology
Publisher
Rockefeller University Press
Published
2009-05-18
Volume
185
Issue
4
Pages
673-684
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
DOI
10.1083/jcb.200809122
PMC
PMC2711563
OpenAlex
W2137960793
Cited by
169
References
2
Citations by year
2026: 4 · 2025: 9 · 2024: 8 · 2023: 4 · 2022: 9 · 2021: 10 · 2020: 11 · 2019: 8 · 2018: 8 · 2017: 16 · 2016: 9 · 2015: 19 · 2014: 10 · 2013: 13 · 2012: 12
FWCI
4.60
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • The Journal of Cell Biology (publishedVersion, cc-by-nc-sa)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
  • Digital Access to Scholarship at Harvard (DASH) (Harvard University) (submittedVersion, cc-by)
Funders
  • National Institutes of Health (DK53056)
  • Harvard Digestive Diseases Center, Harvard University (P30DK034854, DK034854)
  • National Cancer Institute (R01CA092354, CA092354)
  • National Institute of Diabetes and Digestive and Kidney Diseases (DK-070856, R56DK070856, R01DK084424, R01DK070856, R01DK053056, R01 DK53056, R01DK-48370, P30DK034854, R01DK048370, P30DK040561)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
  • Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
Keywords
neonatal Fc receptor, IgG transport, transcytosis, recycling endosomes, epithelial polarity, MYO5B, Rab25, Rab11a, mucosal immunity, lysosomal escape
MeSH terms
rab11 GTP-Binding Proteins, Animals, Cell Compartmentation, Cell Line, Cell Membrane, Dogs, Humans, Immunoglobulin G, Receptors, Fc, Endosomes, Cell Polarity, Myosin Heavy Chains, rab GTP-Binding Proteins, Protein Transport, Myosin Type V

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