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Actin polymerization driven by WASH causes V-ATPase retrieval and vesicle neutralization before exocytosis

Carnell, Michael, Zech, Tobias, Calaminus, Simon D., Ura, Seiji, Hagedorn, Monica, Johnston, Simon A., May, Robin C., Soldati, Thierry, Machesky, Laura M., Insall, Robert H.·2011·Journal of Cell Biology
DOI 10.1083/jcb.201009119PMC PMC3105540
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Abstract

WASP and SCAR homologue (WASH) is a recently identified and evolutionarily conserved regulator of actin polymerization. In this paper, we show that WASH coats mature Dictyostelium discoideum lysosomes and is essential for exocytosis of indigestible material. A related process, the expulsion of the lethal endosomal pathogen Cryptococcus neoformans from mammalian macrophages, also uses WASH-coated vesicles, and cells expressing dominant negative WASH mutants inefficiently expel C. neoformans. D. discoideum WASH causes filamentous actin (F-actin) patches to form on lysosomes, leading to the removal of vacuolar adenosine triphosphatase (V-ATPase) and the neutralization of lysosomes to form postlysosomes. Without WASH, no patches or coats are formed, neutral postlysosomes are not seen, and indigestible material such as dextran is not exocytosed. Similar results occur when actin polymerization is blocked with latrunculin. V-ATPases are known to bind avidly to F-actin. Our data imply a new mechanism, actin-mediated sorting, in which WASH and the Arp2/3 complex polymerize actin on vesicles to drive the separation and recycling of proteins such as the V-ATPase.

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  • Regulation of branched F-actin formation by WASH is needed for proper morphology of the endolysosomal system and endosomal trafficking of a broad range of cargoes.

    “Regulation of branched F-actin formation by WASH is needed for proper morphology of the endolysosomal system and endosomal trafficking of a broad range of cargoes37,39,41–44”

    From Endosomal PI(3)P regulation by the COMMD/CCDC22/CCDC93 (CCC) complex controls membrane protein recycling (2019) · cites this paper as [42]

    The Results and discussion show that WASH-driven actin polymerization is needed for V-ATPase retrieval and exocytosis, and that WASH-null cells develop enlarged vesicles. The Introduction also describes endosomal tubulation and two trafficking pathways in mammalian cells. These passages support a role in endolysosomal morphology and trafficking, but do not establish trafficking of a broad range of cargoes. (LLM, conf=0.86)

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  • The WASH complex functions in endocytic recycling, maintenance of endolysosomal network integrity, exocytosis, cell adhesion, and cell migration.

    “The WASH complex functions in the endocytic recycling of various types of membrane proteins, maintenance of endolysosomal network integrity, exocytosis, cell adhesion, and cell migration (11-23).”

    From Hereditary spastic paraplegia SPG8 mutations impair CAV1-dependent, integrin-mediated cell adhesion (2020) · cites this paper as [14]

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Research works printed but not linked: 35

  1. Alvarez, 2006, Phagosome extrusion and host-cell survival after Cryptococcus neoformans phagocytosis by macrophages, Curr. Biol., 16, 2161, 10.1016/j.cub.2006.09.061 · DOI · Paper page
  2. Bear, 2009, Sorting out endosomes in the WASH, Dev. Cell., 17, 583, 10.1016/j.devcel.2009.10.019 · DOI
  3. Campellone, 2008, WHAMM is an Arp2/3 complex activator that binds microtubules and functions in ER to Golgi transport, Cell., 134, 148, 10.1016/j.cell.2008.05.032 · DOI
  4. Clarke, 2002, Dynamics of the vacuolar H(+)-ATPase in the contractile vacuole complex and the endosomal pathway of Dictyostelium cells, J. Cell Sci., 115, 2893, 10.1242/jcs.115.14.2893 · DOI · Paper page
  5. Clarke, 2010, Retrieval of the vacuolar H-ATPase from phagosomes revealed by live cell imaging, PLoS ONE., 5, e8585, 10.1371/journal.pone.0008585 · DOI
  6. Derivery, 2009, The Arp2/3 activator WASH controls the fission of endosomes through a large multiprotein complex, Dev. Cell., 17, 712, 10.1016/j.devcel.2009.09.010 · DOI · Paper page
  7. Drengk, 2003, A coat of filamentous actin prevents clustering of late-endosomal vacuoles in vivo, Curr. Biol., 13, 1814, 10.1016/j.cub.2003.09.037 · DOI
  8. Duleh, 2010, WASH and the Arp2/3 complex regulate endosome shape and trafficking, Cytoskeleton (Hoboken)., 67, 193, 10.1002/cm.20437 · DOI · Paper page
  9. Gomez, 2009, A FAM21-containing WASH complex regulates retromer-dependent sorting, Dev. Cell., 17, 699, 10.1016/j.devcel.2009.09.009 · DOI · Paper page
  10. Gotthardt, 2002, High-resolution dissection of phagosome maturation reveals distinct membrane trafficking phases, Mol. Biol. Cell., 13, 3508, 10.1091/mbc.E02-04-0206 · DOI
  11. Hagedorn, 2009, Infection by tubercular mycobacteria is spread by nonlytic ejection from their amoeba hosts, Science., 323, 1729, 10.1126/science.1169381 · DOI
  12. Hernandez-Valladares, 2010, Structural characterization of a capping protein interaction motif defines a family of actin filament regulators, Nat. Struct. Mol. Biol., 17, 497, 10.1038/nsmb.1792 · DOI
  13. Holliday, 2000, The amino-terminal domain of the B subunit of vacuolar H+-ATPase contains a filamentous actin binding site, J. Biol. Chem., 275, 32331, 10.1074/jbc.M004795200 · DOI
  14. Humphries, 2002, Direct regulation of Arp2/3 complex activity and function by the actin binding protein coronin, J. Cell Biol., 159, 993, 10.1083/jcb.200206113 · DOI
  15. Ibarra, 2005, Regulation of actin assembly by SCAR/WAVE proteins, Biochem. Soc. Trans., 33, 1243, 10.1042/BST20051243 · DOI
  16. Jefferies, 2008, Function, structure and regulation of the vacuolar (H+)-ATPases, Arch. Biochem. Biophys., 476, 33, 10.1016/j.abb.2008.03.025 · DOI
  17. Jenne, 1998, Targeted gene disruption reveals a role for vacuolin B in the late endocytic pathway and exocytosis, J. Cell Sci., 111, 61, 10.1242/jcs.111.1.61 · DOI
  18. Lee, 2002, Visualization of actin dynamics during macropinocytosis and exocytosis, Traffic., 3, 186, 10.1034/j.1600-0854.2002.030304.x · DOI
  19. Linardopoulou, 2007, Human subtelomeric WASH genes encode a new subclass of the WASP family, PLoS Genet., 3, e237, 10.1371/journal.pgen.0030237 · DOI
  20. Linder, 1999, Wiskott-Aldrich syndrome protein regulates podosomes in primary human macrophages, Proc. Natl. Acad. Sci. USA., 96, 9648, 10.1073/pnas.96.17.9648 · DOI
  21. Ma, 2006, Expulsion of live pathogenic yeast by macrophages, Curr. Biol., 16, 2156, 10.1016/j.cub.2006.09.032 · DOI · Paper page
  22. Maniak, 2001, Fluid-phase uptake and transit in axenic Dictyostelium cells, Biochim. Biophys. Acta., 1525, 197, 10.1016/S0304-4165(01)00105-2 · DOI
  23. Neuhaus, 2002, Morphology and dynamics of the endocytic pathway in Dictyostelium discoideum, Mol. Biol. Cell., 13, 1390, 10.1091/mbc.01-08-0392 · DOI
  24. Parra, 1998, Reversible association between the V1 and V0 domains of yeast vacuolar H+-ATPase is an unconventional glucose-induced effect, Mol. Cell. Biol., 18, 7064, 10.1128/MCB.18.12.7064 · DOI
  25. Pollard, 2007, Regulation of actin filament assembly by Arp2/3 complex and formins, Annu. Rev. Biophys. Biomol. Struct., 36, 451, 10.1146/annurev.biophys.35.040405.101936 · DOI
  26. Qualmann, 2002, Endocytosis and the cytoskeleton, Int. Rev. Cytol., 220, 93, 10.1016/S0074-7696(02)20004-2 · DOI
  27. Rauchenberger, 1997, Coronin and vacuolin identify consecutive stages of a late, actin-coated endocytic compartment in Dictyostelium, Curr. Biol., 7, 215, 10.1016/S0960-9822(97)70093-9 · DOI
  28. Rivero, 2006, Quantitative and microscopic methods for studying the endocytic pathway, Methods Mol. Biol., 346, 423
  29. Steffen, 2004, Sra-1 and Nap1 link Rac to actin assembly driving lamellipodia formation, EMBO J., 23, 749, 10.1038/sj.emboj.7600084 · DOI
  30. Veltman, 2010, WASP family proteins: their evolution and its physiological implications, Mol. Biol. Cell., 21, 2880, 10.1091/mbc.E10-04-0372 · DOI
  31. Veltman, 2009, A new set of small, extrachromosomal expression vectors for Dictyostelium discoideum, Plasmid., 61, 110, 10.1016/j.plasmid.2008.11.003 · DOI
  32. Vitavska, 2003, A novel role for subunit C in mediating binding of the H+-V-ATPase to the actin cytoskeleton, J. Biol. Chem., 278, 18499, 10.1074/jbc.M212844200 · DOI
  33. Voelz, 2010, Automated analysis of cryptococcal macrophage parasitism using GFP-tagged cryptococci, PLoS ONE., 5, e15968, 10.1371/journal.pone.0015968 · DOI
  34. Wienke, 2006, Vacuolin, a flotillin/reggie-related protein from Dictyostelium oligomerizes for endosome association, Eur. J. Cell Biol., 85, 991, 10.1016/j.ejcb.2006.04.010 · DOI
  35. Yamaguchi, 2005, Molecular mechanisms of invadopodium formation: the role of the N-WASP-Arp2/3 complex pathway and cofilin, J. Cell Biol., 168, 441, 10.1083/jcb.200407076 · DOI

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Metadata

Authors
Carnell, M., Zech, T., Calaminus, S. D., Ura, S., Hagedorn, M., Johnston, S. A., May, R. C., Soldati, T., Machesky, L. M., & Insall, R. H.
Venue
Journal of Cell Biology
Publisher
Rockefeller University Press
Published
2011-05-23
Volume
193
Issue
5
Pages
831-839
ISSN
  • 1540-8140
  • 0021-9525
DOI
10.1083/jcb.201009119
PMC
PMC3105540
Cited by
159
References
0
Language
en

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