The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Pannexin 3 functions as an ER Ca2+ channel, hemichannel, and gap junction to promote osteoblast differentiation
Open accessAbstract
The pannexin proteins represent a new gap junction family. However, the cellular functions of pannexins remain largely unknown. Here, we demonstrate that pannexin 3 (Panx3) promotes differentiation of osteoblasts and ex vivo growth of metatarsals. Panx3 expression was induced during osteogenic differentiation of C2C12 cells and primary calvarial cells, and suppression of this endogenous expression inhibited differentiation. Panx3 functioned as a unique Ca2+ channel in the endoplasmic reticulum (ER), which was activated by purinergic receptor/phosphoinositide 3-kinase (PI3K)/Akt signaling, followed by activation of calmodulin signaling for differentiation. Panx3 also formed hemichannels that allowed release of ATP into the extracellular space and activation of purinergic receptors with the subsequent activation of PI3K–Akt signaling. Panx3 also formed gap junctions and propagated Ca2+ waves between cells. Blocking the Panx3 Ca2+ channel and gap junction activities inhibited osteoblast differentiation. Thus, Panx3 appears to be a new regulator that promotes osteoblast differentiation by functioning as an ER Ca2+ channel and a hemichannel, and by forming gap junctions.
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Cites
The versatility and universality of calcium signalling
Berridge, M. J., Lipp, P., & Bootman, M. D. (2000) · Nature Reviews Molecular Cell Biology · doi:10.1038/35036035 · Cited by 5,701
Akt Enhances Mdm2-mediated Ubiquitination and Degradation of p53
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“Akt-mediated MDM2 phosphorylation leads to p53 ubiquitination (Ogawara et al., 2002).”
Biswas, G., Adebanjo, O. A., Freedman, B. D., Anandatheerthavarada, H. K., Vijayasarathy, C., Zaidi, M., Kotlikoff, M., & Avadhani, N. G. (1999) · The EMBO Journal · doi:10.1093/emboj/18.3.522 · Cited by 369 · cited 2×
“, 2001), whereas RyRs are not expressed (Fig. S1 C; Biswas et al., 1999).”
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Cited by
Zhou, T., Gao, B., Fan, Y., Liu, Y., Feng, S., Cong, Q., Zhang, X., Zhou, Y., Yadav, P. S., Lin, J., et al. (2020) · eLife · doi:10.7554/elife.52779 · Cited by 392
Intercellular Ca2+Waves: Mechanisms and Function
Leybaert, L., & Sanderson, M. J. (2012) · Physiological Reviews · doi:10.1152/physrev.00029.2011 · Cited by 331
Metadata
- Authors
- Ishikawa, M., Iwamoto, T., Nakamura, T., Doyle, A., Fukumoto, S., & Yamada, Y.
- Venue
- Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2011-06-20
- Volume
- 193
- Issue
- 7
- Pages
- 1257-1274
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2138977093
- Cited by
- 226
- References
- 4
- FWCI
- 8.35
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Ishikawa, M., Iwamoto, T., Nakamura, T., Doyle, A., Fukumoto, S., & Yamada, Y. (2011). Pannexin 3 functions as an ER Ca2+ channel, hemichannel, and gap junction to promote osteoblast differentiation. Journal of Cell Biology. https://doi.org/10.1083/jcb.201101050
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