The DOI matches its Crossref record.
Nuclear translation visualized by ribosome-bound nascent chain puromycylation
Open accessAbstract
Whether protein translation occurs in the nucleus is contentious. To address this question, we developed the ribopuromycylation method (RPM), which visualizes translation in cells via standard immunofluorescence microscopy. The RPM is based on ribosome-catalyzed puromycylation of nascent chains immobilized on ribosomes by antibiotic chain elongation inhibitors followed by detection of puromycylated ribosome-bound nascent chains with a puromycin (PMY)-specific monoclonal antibody in fixed and permeabilized cells. The RPM correlates localized translation with myriad processes in cells and can be applied to any cell whose translation is sensitive to PMY. In this paper, we use the RPM to provide evidence for translation in the nucleoplasm and nucleolus, which is regulated by infectious and chemical stress.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
G3BP–Caprin1–USP10 complexes mediate stress granule condensation and associate with 40S subunits
Kedersha, N., Panas, M. D., Achorn, C. A., Lyons, S., Tisdale, S., Hickman, T., Thomas, M., Lieberman, J., McInerney, G. M., Ivanov, P., et al. (2016) · Journal of Cell Biology · doi:10.1083/jcb.201508028 · Cited by 659
V'kovski, P., Gerber, M., Kelly, J., Pfaender, S., Ebert, N., Braga Lagache, S., Simillion, C., Portmann, J., Stalder, H., Gaschen, V., et al. (2019) · eLife · doi:10.7554/elife.42037 · Cited by 186
Dong, Z., Chen, W., Chen, C., Wang, H., Cui, W., Tan, Z., Robinson, H., Gao, N., Luo, B., Zhang, L., et al. (2020) · Neuron · doi:10.1016/j.neuron.2019.10.035 · Cited by 109
Aviner, R., Geiger, T., & Elroy-Stein, O. (2014) · Nature Protocols · doi:10.1038/nprot.2014.051 · Cited by 76
Cellular maintenance of nuclear protein homeostasis.
Gallagher, P. S., Oeser, M. L., Abraham, A.-C., Kaganovich, D., & Gardner, R. G. (2014) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-013-1530-y · Cited by 22
Metadata
- Authors
- David, A., Dolan, B. P., Hickman, H. D., Knowlton, J. J., Clavarino, G., Pierre, P., Bennink, J. R., & Yewdell, J. W.
- Venue
- Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2012-04-02
- Volume
- 197
- Issue
- 1
- Pages
- 45-57
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2101990520
- Cited by
- 295
- References
- 0
- FWCI
- 8.71
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Nuclear translation visualized by ribosome-bound nascent chain puromycylation. https://app.cochat.ai/papers/10.1083/jcb.201112145
- The paper
- David, A., Dolan, B. P., Hickman, H. D., Knowlton, J. J., Clavarino, G., Pierre, P., Bennink, J. R., & Yewdell, J. W. (2012). Nuclear translation visualized by ribosome-bound nascent chain puromycylation. Journal of Cell Biology. https://doi.org/10.1083/jcb.201112145
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
