The DOI matches its Crossref record.
A ROUTINE TECHNIQUE FOR DOUBLE-STAINING ULTRATHIN SECTIONS USING URANYL AND LEAD SALTS
Open accessAbstract
For more than 2 years, a double-staining of sections of OsO 4-fixed and Epon-embedded tissues with uranyl acetate followed by lead hydroxide has been routine technique in this laboratory. It was found to give substantially higher contrast, thus permitting the use of thinner sections. It facilitated focusing and routinely provided clearer pictures of cell fine structure than could be obtained with either stain alone. However, the formation of a fine precipitate frequently marred the sections, and this difficulty could never be satisfactorily controlled. Apparently, it is caused by the uranyl acetate solution which is not stable at the pH and concentration used. Decreasing the concentration and/or the pH of the solution caused an undesirable loss of staining intensity. Therefore, a number of different uranyl salts were tested as a substitute for uranyl acetate in the procedure, and one of them, uranyl magnesium acetate, has been found to give consistently good results.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Graus-Porta, D., Blaess, S., Senften, M., Littlewood-Evans, A., Damsky, C., Huang, Z., Orban, P., Klein, R., Schittny, J. C., & Müller, U. (2001) · Neuron · doi:10.1016/s0896-6273(01)00374-9 · Cited by 559
Nair, P. N. R., Duncan, H. F., Pitt Ford, T. R., & Luder, H. U. (2008) · International Endodontic Journal · doi:10.1111/j.1365-2591.2007.01329.x · Cited by 469
Peterson, J. B., & Ris, H. (1976) · Journal of Cell Science · doi:10.1242/jcs.22.2.219 · Cited by 278
PERMEABILITY OF INTESTINAL CAPILLARIES
Simionescu, N., Simionescu, M., & Palade, G. E. (1972) · The Journal of Cell Biology · doi:10.1083/jcb.53.2.365 · Cited by 275
Venkateshappa, C., Harish, G., Mahadevan, A., Srinivas Bharath, M. M., & Shankar, S. K. (2012) · Neurochemical Research · doi:10.1007/s11064-012-0755-8 · Cited by 232
Metadata
- Authors
- Frasca, J. M., & Parks, V. R.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1965-04-01
- Volume
- 25
- Issue
- 1
- Pages
- 157-161
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2060253502
- Cited by
- 445
- References
- 0
- FWCI
- 7.72
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: A ROUTINE TECHNIQUE FOR DOUBLE-STAINING ULTRATHIN SECTIONS USING URANYL AND LEAD SALTS. https://app.cochat.ai/papers/10.1083/jcb.25.1.157
- The paper
- Frasca, J. M., & Parks, V. R. (1965). A ROUTINE TECHNIQUE FOR DOUBLE-STAINING ULTRATHIN SECTIONS USING URANYL AND LEAD SALTS. The Journal of Cell Biology. https://doi.org/10.1083/jcb.25.1.157
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
