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Cited by 179

The DOI matches its Crossref record.

STRUCTURE OF MEMBRANE HOLES IN OSMOTIC AND SAPONIN HEMOLYSIS

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Seeman, P., Cheng, D., Iles, G. H.·1973·The Journal of Cell Biology
DOI 10.1083/jcb.56.2.519
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Abstract

Serial section electron microscopy of hemolysing erythrocytes (fixed at 12 s after the onset of osmotic hemolysis) revealed long slits and holes in the membrane, extending to around 1 µm in length. Many but not all of the slits and holes (about 100–1000 Å wide) were confluent with one another. Ferritin and colloidal gold (added after fixation) only permeated those cells containing membrane defects. No such large holes or slits were seen in saponin-treated erythrocytes, and the membrane was highly invaginated, giving the ghost a scalloped outline. Freeze-etch electron microscopy of saponin-treated membranes revealed 40–50 Å-wide pits in the extracellular surface of the membrane. If these pits represent regions from which cholesterol was extracted, then cholesterol is uniformly distributed over the entire erythrocyte membrane.

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Metadata

Authors
Seeman, P., Cheng, D., & Iles, G. H.
Venue
The Journal of Cell Biology
Publisher
Rockefeller University Press
Published
1973-02-01
Volume
56
Issue
2
Pages
519-527
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
DOI
10.1083/jcb.56.2.519
OpenAlex
W2131161781
Cited by
179
References
0
FWCI
3.26
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: STRUCTURE OF MEMBRANE HOLES IN OSMOTIC AND SAPONIN HEMOLYSIS. https://app.cochat.ai/papers/10.1083/jcb.56.2.519
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Seeman, P., Cheng, D., & Iles, G. H. (1973). STRUCTURE OF MEMBRANE HOLES IN OSMOTIC AND SAPONIN HEMOLYSIS. The Journal of Cell Biology. https://doi.org/10.1083/jcb.56.2.519

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