The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
Viscometric analysis of the gelation of Acanthamoeba extracts and purification of two gelation factors.
Open accessAbstract
We have studied the kinetics of the gelation process that occurs upon warming cold extracts of Acanthamoeba using a low-shear falling ball assay. We find that the reaction has at least two steps, requires 0.5 mM ATP and 1.5 mM MgCl2, and is inhibited by micromolar Ca++. The optimum pH is 7.0 and temperature, 25 degrees-30 degrees C. The rate of the reaction is increased by cold preincubation with both MgCl2 and ATP. Nonhydrolyzable analogues of ATP will not substitute for ATP either in this "potentiation reaction" or in the gelation process. Either of two purified or any one of four partially purified Acanthamoeba proteins will cross-link purified actin to form a gel, but none can account for the dependence of the reaction in the crude extract on Mg-ATP or its regulation by Ca++. This suggests that the extract contains, in addition to actin-cross-linking proteins, factors dependent on Mg-ATP and Ca++ that regulate the gelation process.
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Citations
Cites
Hartree, E. F. (1972) · Analytical Biochemistry · doi:10.1016/0003-2697(72)90094-2 · Cited by 5,516
The Regulation of Rabbit Skeletal Muscle Contraction
Spudich, J. A., & Watt, S. (1971) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)62016-2 · Cited by 4,493
Actin polymerizability is influenced by profilin, a low molecular weight protein in non-muscle cells
Carlsson, L., Nyström, L.-E., Sundkvist, I., Markey, F., & Lindberg, U. (1977) · Journal of Molecular Biology · doi:10.1016/0022-2836(77)90166-8 · Cited by 701
Cytoplasmic Structure and Contractility in Amoeboid Cells
Taylor, D. L., & Condeelis, J. S. (1979) · International Review of Cytology · doi:10.1016/s0074-7696(08)61821-5 · Cited by 339
Stossel, T. P., & Hartwig, J. H. (1976) · The Journal of cell biology · doi:10.1083/jcb.68.3.602 · Cited by 287
Preparation and purification of polymerized actin from sea urchin egg extracts.
Kane, R. E. (1975) · The Journal of cell biology · doi:10.1083/jcb.66.2.305 · Cited by 219
THE CONTRACTILE BASIS OF AMOEBOID MOVEMENT
Taylor, D. L., Condeelis, J. S., Moore, P. L., & Allen, R. D. (1973) · The Journal of Cell Biology · doi:10.1083/jcb.59.2.378 · Cited by 217
The role of actin in the temperature-dependent gelation and contraction of extracts of Acanthamoeba.
Pollard, T. D. (1976) · The Journal of cell biology · doi:10.1083/jcb.68.3.579 · Cited by 215
Self-citationCondeelis, J. S., & Taylor, D. L. (1977) · The Journal of Cell Biology · doi:10.1083/jcb.74.3.901 · Cited by 198
Separation and interaction of the major components of sea urchin actin gel
Bryan, J., & Kane, R. E. (1978) · Journal of Molecular Biology · doi:10.1016/0022-2836(78)90345-5 · Cited by 194
Cited by
Functional significance of cell volume regulatory mechanisms
LANG, F., BUSCH, G. L., RITTER, M., VÖLKL, H., WALDEGGER, S., GULBINS, E., & HÄUSSINGER, D. (1998) · Physiological Reviews · doi:10.1152/physrev.1998.78.1.247 · Cited by 1,891
Hartwig, J. H., Thelen, M., Resen, A., Janmey, P. A., Nairn, A. C., & Aderem, A. (1992) · Nature · doi:10.1038/356618a0 · Cited by 730
Mechanics of Living Cells Measured by Laser Tracking Microrheology
Yamada, S., Wirtz, D., & Kuo, S. C. (2000) · Biophysical Journal · doi:10.1016/s0006-3495(00)76725-7 · Cited by 517
MacLean-Fletcher, S., & Pollard, T. D. (1980) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(80)91175-4 · Cited by 465
Self-citationHemostatic, inflammatory, and fibroblast responses are blunted in mice lacking gelsolin
Witke, W., Sharpe, A. H., Hartwig, J. H., Azuma, T., Stossel, T. P., & Kwiatkowski, D. J. (1995) · Cell · doi:10.1016/0092-8674(95)90369-0 · Cited by 431
Metadata
- Authors
- MacLean-Fletcher, S. D., & Pollard, T. D.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1980-05-01
- Volume
- 85
- Issue
- 2
- Pages
- 414-428
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W1988312243
- Cited by
- 340
- References
- 15
- FWCI
- 12.23
- Type
- journal article
- Language
- en
- Open access
- bronze
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- MacLean-Fletcher, S. D., & Pollard, T. D. (1980). Viscometric analysis of the gelation of Acanthamoeba extracts and purification of two gelation factors. The Journal of cell biology. https://doi.org/10.1083/jcb.85.2.414
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
