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Serial propagation of human endothelial cells in vitro.
Open accessAbstract
Human umbilical vein (HUV) endothelial cells were grown for 15 to 21 passages at a split ratio of 1:5 (at least 27 population doublings) on a human fibronectin (HFN) matrix in Medium 199 supplemented with fetal bovine serum (FBS) and endothelial-cell growth factor (ECGF). This system also permitted the growth of HUV endothelial cells at cell densities as low as 1.25 cells/cm2. In addition to delaying the premature senescence of HUV endothelial cells, ECGF also reduced the serum requirement for low-density HUV endothelial-cell growth; 2.5% serum and ECGF yields half-maximum growth as compared to high serum controls. Significant HUV endothelial-cell growth was also observed in medium supplemented with either ovine hypophysectomized (HYPOX) serum, plasma-derived serum (PDS), or HYPOX-PDS in the presence of ECGF, suggesting that neither the pituitary nor the platelet contributes to HUV endothelial-cell growth.
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Cited by
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Dimri, G. P., Lee, X., Basile, G., Acosta, M., Scott, G., Roskelley, C., Medrano, E. E., Linskens, M., Rubelj, I., & Pereira-Smith, O. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.20.9363 · Cited by 7,477
DeCaprio, J. A., Ludlow, J. W., Lynch, D., Furukawa, Y., Griffin, J., Piwnica-Worms, H., Huang, C.-M., & Livingston, D. M. (1989) · Cell · doi:10.1016/0092-8674(89)90507-2 · Cited by 897
Ke, Y., Li, D., Zhao, M., Liu, C., Liu, J., Zeng, A., Shi, X., Cheng, S., Pan, B., Zheng, L., et al. (2018) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2018.01.007 · Cited by 285
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Metadata
- Authors
- Maciag, T., Hoover, G. A., Stemerman, M. B., & Weinstein, R.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1981-11-01
- Volume
- 91
- Issue
- 2
- Pages
- 420-426
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2159329277
- Cited by
- 342
- References
- 0
- FWCI
- 6.77
- Type
- journal article
- Language
- en
- Open access
- green
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- Maciag, T., Hoover, G. A., Stemerman, M. B., & Weinstein, R. (1981). Serial propagation of human endothelial cells in vitro. The Journal of cell biology. https://doi.org/10.1083/jcb.91.2.420
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