The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum.
Open accessAbstract
A rapid and simple method for the isolation of membranes from subcellular organelles is described. The procedure consists of diluting the organelles in ice-cold 100 mM Na2CO3 followed by centrifugation to pellet the membranes. Closed vesicles are converted to open membrane sheets, and content proteins and peripheral membrane proteins are released in soluble form. Here we document the method by applying it to various subfractions of a rat liver microsomal fraction, prepared by continuous density gradient centrifugation according to Beaufay et al. (1974, J. Cell Biol. 61:213-231). The results confirm and extend those of previous investigators on the distribution of enzymes and proteins among the membranes of the smooth and rough endoplasmic reticulum. In the accompanying paper (1982, J. Cell Biol. 93:103-110) the procedure is applied to peroxisomes and mitochondria.
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Citations
Cites
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751
Cited by
Brown, D. A., & Rose, J. K. (1992) · Cell · doi:10.1016/0092-8674(92)90189-j · Cited by 2,921
Nile red: a selective fluorescent stain for intracellular lipid droplets.
Greenspan, P., Mayer, E. P., & Fowler, S. D. (1985) · The Journal of cell biology · doi:10.1083/jcb.100.3.965 · Cited by 2,543
Self-citationMammalian Sir2 homolog SIRT3 regulates global mitochondrial lysine acetylation
Lombard, D. B., Alt, F. W., Cheng, H.-L., Bunkenborg, J., Streeper, R. S., Mostoslavsky, R., Kim, J., Yancopoulos, G., Valenzuela, D., Murphy, A., et al. (2007) · Molecular and Cellular Biology · doi:10.1128/mcb.01636-07 · Cited by 1,216
Emmanouilidou, E., Melachroinou, K., Roumeliotis, T., Garbis, S. D., Ntzouni, M., Margaritis, L. H., Stefanis, L., & Vekrellis, K. (2010) · The Journal of Neuroscience · doi:10.1523/jneurosci.5699-09.2010 · Cited by 1,115
“To remove non-integral membrane proteins, exosomes were treated with Na2CO3 100 mM, pH 11, for 30 min at 4°C (Fujiki et al., 1982).”
MethodSpectrofluorometric studies of the lipid probe, nile red.
Greenspan, P., & Fowler, S. D. (1985) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)34307-8 · Cited by 980
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Metadata
- Authors
- Fujiki, Y., Hubbard, A. L., Fowler, S., & Lazarow, P. B.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1982-04-01
- Volume
- 93
- Issue
- 1
- Pages
- 97-102
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2160107586
- Cited by
- 1,751
- References
- 1
- FWCI
- 9.84
- Type
- journal article
- Language
- en
- Open access
- green
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- Fujiki, Y., Hubbard, A. L., Fowler, S., & Lazarow, P. B. (1982). Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum. The Journal of cell biology. https://doi.org/10.1083/jcb.93.1.97
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