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DETECTION OF SPECIFIC ANTIGEN IN SV40-TRANSFORMED CELLS BY IMMUNOFLUORESCENCE

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Pope, John H., Rowe, Wallace P.·1964·The Journal of Experimental Medicine

Abstract

With an immunofluorescent technique involving the use of serum of hamsters with SV40 tumors, nuclear fluorescence was detected in each of five cell lines, derived from four mammalian species, transformed by SV40 virus. Essentially all nuclei, including those of multinuclear cells, were fluorescent-stainable. Serum of hamsters bearing SV40 tumors was also found to give nuclear fluorescence in susceptible cells (AGMK or BSC-1) acutely infected with SV40 virus. These findings provide further evidence that cellular incorporation of the SV40 viral genome, with partial expression of the genome by synthesis of at least one virus-specific antigen, is an integral property of all SV40 transformed cells.

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Metadata

Authors
Pope, J. H., & Rowe, W. P.
Venue
The Journal of Experimental Medicine
Published
1964-08-01
Volume
120
Issue
2
Pages
121-128
ISSN
  • 0022-1007 (ISSN-L)
  • 1540-9538
OpenAlex
W2008107181
Cited by
420
References
0
FWCI
41.46
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: DETECTION OF SPECIFIC ANTIGEN IN SV40-TRANSFORMED CELLS BY IMMUNOFLUORESCENCE. https://app.cochat.ai/papers/10.1084/jem.120.2.121
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Pope, J. H., & Rowe, W. P. (1964). DETECTION OF SPECIFIC ANTIGEN IN SV40-TRANSFORMED CELLS BY IMMUNOFLUORESCENCE. The Journal of Experimental Medicine. https://doi.org/10.1084/jem.120.2.121

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.