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Form variation in Escherichia coli K1: determined by O-acetylation of the capsular polysaccharide.
Open accessAbstract
The chemical basis for the alternating antigenic change called form variation noted for the Escherichia coli K1-capsular polysaccharide has been shown by 13C nuclear magnetic resonance to be a result of random O-acetylation of C7 and C9 carbons of the alpha-2-8-linked sialic acid homopolymer. A serologic method (antiserum agar) was developed to identify and isolate the form variants. The O-acetyl positive and O-acetyl negative K1 polysaccharides had unique biochemical and immunologic properties. The O-acetyl-positive variants resisted neuraminidase hydrolysis in contrast to the susceptibility of the O-acetyl negative variant to this enzyme. In addition, O-acetylation altered the antigenicity of the O-acetyl polysaccharides. When injected as whole organisms, O-acetyl positive organisms produced anti-K1 -antibodies in rabbits specific for this polysaccharide variant. O-acetyl negative organisms were comparatively less immunogenic; however, antibodies induced by these organisms reacted with both K1 polysaccharide variants. Burros, injected with either variant, produced antibodies reactive with both K1 polysaccharides.
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Citations
Cited by
2 Serotyping of Escherichia coli
Ørskov, F., & Ørskov, I. (1984) · Methods in Microbiology · doi:10.1016/s0580-9517(08)70447-1 · Cited by 579
Masquerading microbial pathogens: capsular polysaccharides mimic host-tissue molecules
Cress, B. F., Englaender, J. A., He, W., Kasper, D., Linhardt, R. J., & Koffas, M. A. G. (2014) · FEMS Microbiology Reviews · doi:10.1111/1574-6976.12056 · Cited by 282
“Many K1 CPS strains have been found to possess PSA that has been O-acetylated at the C7 or C9 hydroxyl group (Orskov et al., 1979).”
BackgroundVANN, W. F., SCHMIDT, M. A., JANN, B., & JANN, K. (1981) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1981.tb05343.x · Cited by 272
Yoshida, Y., Yang, J., Peaker, P.-E., Kato, H., Bush, C. A., & Cisar, J. O. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m801412200 · Cited by 30
Going for baroque at the Escherichia coli K1 cell surface
King, M. R., Steenbergen, S. M., & Vimr, E. R. (2007) · Trends in Microbiology · doi:10.1016/j.tim.2007.03.006 · Cited by 20
Metadata
- Authors
- Orskov, F., Orskov, I., Sutton, A., Schneerson, R., Lin, W., Egan, W., Hoff, G. E., & Robbins, J. B.
- Venue
- The Journal of experimental medicine
- Publisher
- Rockefeller University Press
- Published
- 1979-03-01
- Volume
- 149
- Issue
- 3
- Pages
- 669-685
- ISSN
- 0022-1007 (ISSN-L)
- 1540-9538
- OpenAlex
- W2162242806
- Cited by
- 207
- References
- 0
- FWCI
- 5.47
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Orskov, F., Orskov, I., Sutton, A., Schneerson, R., Lin, W., Egan, W., Hoff, G. E., & Robbins, J. B. (1979). Form variation in Escherichia coli K1: determined by O-acetylation of the capsular polysaccharide. The Journal of experimental medicine. https://doi.org/10.1084/jem.149.3.669
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