Activation of basophil and mast cell histamine release by eosinophil granule major basic protein.
Open accessAbstract
Major basic protein (MBP) is a primary constituent of eosinophil granules. In this report, we demonstrate that MBP from human eosinophil granules initiates a nonlytic histamine release from human leukocytes. A direct effect of MBP on basophils was confirmed using purified human basophils. The kinetics of release were similar to those reported for poly-L-arginine, although MBP was less potent than poly-L-arginine of similar molecular weight. Reduction and alkylation of MBP diminished both the potency and efficacy of the molecule. Native MBP also stimulated histamine secretion from purified rat peritoneal mast cells in a manner characteristic of other polycations. These results emphasize the bidirectional nature of the basophil/mast cell-eosinophil regulatory axis.
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Many of the downregulated proteins in TET2-CH are markers of other haematopoietic lineages, such as KIR2DL2 and KIR2DL3, and PRG2 and PRG3.
“Many of the downregulated proteins are markers of other haematopoietic lineages, such as KIR2DL2 and KIR2DL3 (natural killer cell activation)52, and PRG2 and PRG3 (constituents of eosinophil granules also involved in basophil stimulation)53,54.”
From Rare variant associations with plasma protein levels in the UK Biobank (2023) · cites this paper as [53]
The introduction (p. 1981) identifies major basic protein as a constituent of eosinophil granules, and the discussion (p. 1988–1989) reports its activation of human basophils. The cited paper does not identify PRG2 or PRG3 by those names or establish the stated role for both proteins; the downregulation finding belongs to the citing study, not this citation. (LLM, conf=0.84)
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Rare variant associations with plasma protein levels in the UK Biobank
Dhindsa, R. S., Burren, O. S., Sun, B. B., Prins, B. P., Matelska, D., Wheeler, E., Mitchell, J., Oerton, E., Hristova, V. A., Smith, K. R., et al. (2023) · Nature · doi:10.1038/s41586-023-06547-x · Cited by 193 · see citing works
Localization of eosinophil granule major basic protein in human basophils.
Ackerman, S. J., Kephart, G. M., Habermann, T. M., Greipp, P. R., & Gleich, G. J. (1983) · The Journal of experimental medicine · doi:10.1084/jem.158.3.946 · Cited by 99 · see citing works
Self-citationNew insights into the role of the mast cell in asthma.
Brightling, C. E., Bradding, P., Pavord, I. D., & Wardlaw, A. J. (2003) · Clinical & Experimental Allergy · doi:10.1046/j.1365-2222.2003.01636.x · Cited by 97 · see citing works
Mast-cell responses in the development of asthma
Bingham, C. O., & Austen, K. F. (2000) · Journal of Allergy and Clinical Immunology · doi:10.1016/s0091-6749(00)90056-3 · Cited by 80 · see citing works
Increases of plasma eosinophil major basic protein levels late in pregnancy predict onset of labor.
Wasmoen, T. L., Coulam, C. B., Leiferman, K. M., & Gleich, G. J. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.9.3029 · Cited by 41 · see citing works
Self-citationEvidence of eosinophil granule major basic protein in human placenta.
Wasmoen, T. L., McKean, D. J., Benirschke, K., Coulam, C. B., & Gleich, G. J. (1989) · The Journal of experimental medicine · doi:10.1084/jem.170.6.2051 · Cited by 29 · see citing works
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- Authors
- O'Donnell, M. C., Ackerman, S. J., Gleich, G. J., & Thomas, L. L.
- Affiliations
- Rush University (US)
- Venue
- The Journal of experimental medicine
- Publisher
- Rockefeller University Press
- Published
- 1983-06-01
- Volume
- 157
- Issue
- 6
- Pages
- 1981-1991
- ISSN
- 0022-1007 (ISSN-L)
- 1540-9538
- PMC
- PMC2187055
- OpenAlex
- W2187357944
- Cited by
- 321
- References
- 0
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 2 · 2023: 1 · 2022: 5 · 2021: 5 · 2020: 4 · 2019: 7 · 2018: 5 · 2017: 2 · 2016: 2 · 2015: 3 · 2014: 1 · 2013: 4 · 2012: 7
- FWCI
- 5.46
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- The Journal of Experimental Medicine (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Mast cells and histamine
- Health Sciences › Medicine › Physiology › Asthma and respiratory diseases
- Health Sciences › Medicine › Immunology and Allergy › Allergic Rhinitis and Sensitization
- Keywords
- major basic protein, eosinophil granules, histamine release, basophils, mast cells, cationic polymers, poly-L-arginine, protein alkylation, protein reduction
- MeSH terms
- Animals, Basophils, Blood Proteins, Cytoplasmic Granules, Eosinophils, Histamine, Humans, Kinetics, Male, Mast Cells, Peptides, Rats, Inbred Strains, Ribonucleases, Structure-Activity Relationship, Eosinophil Granule Proteins, Rats
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