The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Quantitative Analysis Reveals Expansion of Human Hematopoietic Repopulating Cells After Short-term Ex Vivo Culture
Open accessAbstract
Ex vivo culture of human hematopoietic cells is a crucial component of many therapeutic applications. Although current culture conditions have been optimized using quantitative in vitro progenitor assays, knowledge of the conditions that permit maintenance of primitive human repopulating cells is lacking. We report that primitive human cells capable of repopulating nonobese diabetic (NOD)/severe combined immunodeficiency (SCID) mice (SCID-repopulating cells; SRC) can be maintained and/or modestly increased after culture of CD34+CD38− cord blood cells in serum-free conditions. Quantitative analysis demonstrated a 4- and 10-fold increase in the number of CD34+CD38− cells and colony-forming cells, respectively, as well as a 2- to 4-fold increase in SRC after 4 d of culture. However, after 9 d of culture, all SRC were lost, despite further increases in total cells, CFC content, and CD34+ cells. These studies indicate that caution must be exercised in extending the duration of ex vivo cultures used for transplantation, and demonstrate the importance of the SRC assay in the development of culture conditions that support primitive cells.
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1 retracted
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RetractedThe Basic Science of Gene Therapy
Mulligan, R. C. (1993) · Science · doi:10.1126/science.8493530 · Cited by 1,741
Purification of primitive human hematopoietic cells capable of repopulating immune-deficient mice
Bhatia, M., Wang, J. C. Y., Kapp, U., Bonnet, D., & Dick, J. E. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.10.5320 · Cited by 850
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Cytokine Stimulation of Multilineage Hematopoiesis from Immature Human Cells Engrafted in SCID Mice
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Self-citationA functional comparison of CD34 + CD38- cells in cord blood and bone marrow
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Cited by
Delaney, C., Heimfeld, S., Brashem-Stein, C., Voorhies, H., Manger, R. L., & Bernstein, I. D. (2010) · Nature Medicine · doi:10.1038/nm.2080 · Cited by 771
Sonic hedgehog induces the proliferation of primitive human hematopoietic cells via BMP regulation
Bhardwaj, G., Murdoch, B., Wu, D., Baker, D. P., Williams, K. P., Chadwick, K., Ling, L. E., Karanu, F. N., & Bhatia, M. (2001) · Nature Immunology · doi:10.1038/84282 · Cited by 677
Self-citationDemaison, C., Parsley, K., Brouns, G., Scherr, M., Battmer, K., Kinnon, C., Grez, M., & Thrasher, A. J. (2002) · Human Gene Therapy · doi:10.1089/10430340252898984 · Cited by 486
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Metadata
- Authors
- Bhatia, M., Bonnet, D., Kapp, U., Wang, J. C. Y., Murdoch, B., & Dick, J. E.
- Venue
- The Journal of Experimental Medicine
- Publisher
- Rockefeller University Press
- Published
- 1997-08-18
- Volume
- 186
- Issue
- 4
- Pages
- 619-624
- ISSN
- 0022-1007 (ISSN-L)
- 1540-9538
- OpenAlex
- W2054031422
- Cited by
- 386
- References
- 22
- FWCI
- 18.89
- Type
- journal article
- Language
- en
- Open access
- green
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- Bhatia, M., Bonnet, D., Kapp, U., Wang, J. C. Y., Murdoch, B., & Dick, J. E. (1997). Quantitative Analysis Reveals Expansion of Human Hematopoietic Repopulating Cells After Short-term Ex Vivo Culture. The Journal of Experimental Medicine. https://doi.org/10.1084/jem.186.4.619
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