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Endothelial Cell “Memory” of Inflammatory Stimulation: Human Venular Endothelial Cells Store Interleukin 8 in Weibel-Palade Bodies
Open accessAbstract
The expression and secretion of interleukin (IL)-8, the prototype member of the C-X-C subfamily of chemokines, can be induced by diverse inflammatory stimuli in many cells, including endothelial cells (EC). Upon de novo synthesis, IL-8 localizes intracellularly in the Golgi apparatus, from where it is secreted. In addition to this constitutive secretory pathway, we describe a depot storage and separate regulated secretory pathway of IL-8 in EC. The prolonged stimulation of primary human EC with inflammatory mediators resulted in the accumulation of IL-8 in Weibel-Palade bodies, where it colocalized with von Willebrand factor. IL-8 was retained in these storage organelles for several days after the removal of the stimulus and could be released by EC secretagogues such as phorbol myristate acetate, the calcium ionophore A23187, and histamine. These findings suggest that storage of IL-8 in Weibel-Palade bodies may serve as the EC “memory” of a preceding inflammatory insult, which then enables the cells to secrete IL-8 immediately without de novo protein synthesis.
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Cited by
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The blood-brain barrier, chemokines and multiple sclerosis
Holman, D. W., Klein, R. S., & Ransohoff, R. M. (2011) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2010.07.019 · Cited by 247
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Nabah, Y. N. A., Mateo, T., Estellés, R., Mata, M., Zagorski, J., Sarau, H., Cortijo, J., Morcillo, E. J., Jose, P. J., & Sanz, M.-J. (2004) · Circulation · doi:10.1161/01.cir.0000148824.93600.f3 · Cited by 124
Ferrari, S. M., Ragusa, F., Paparo, S. R., Nasini, F., Nardi, M., Franceschini, S. S., Fallahi, P., & Antonelli, A. (2019) · Autoimmunity Reviews · doi:10.1016/j.autrev.2019.05.004 · Cited by 26
Metadata
- Authors
- Wolff, B., Burns, A. R., Middleton, J., & Rot, A.
- Venue
- The Journal of Experimental Medicine
- Publisher
- Rockefeller University Press
- Published
- 1998-11-02
- Volume
- 188
- Issue
- 9
- Pages
- 1757-1762
- ISSN
- 0022-1007 (ISSN-L)
- 1540-9538
- OpenAlex
- W1989237374
- Cited by
- 219
- References
- 0
- FWCI
- 3.94
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Endothelial Cell “Memory” of Inflammatory Stimulation: Human Venular Endothelial Cells Store Interleukin 8 in Weibel-Palade Bodies. https://app.cochat.ai/papers/10.1084/jem.188.9.1757
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- Wolff, B., Burns, A. R., Middleton, J., & Rot, A. (1998). Endothelial Cell “Memory” of Inflammatory Stimulation: Human Venular Endothelial Cells Store Interleukin 8 in Weibel-Palade Bodies. The Journal of Experimental Medicine. https://doi.org/10.1084/jem.188.9.1757
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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