mtDNA Analysis of a Prehistoric Oneota Population: Implications for the Peopling of the New World
Open accessAbstract
mtDNA was successfully extracted from 108 individuals from the Norris Farms Oneota, a prehistoric Native American population, to compare the mtDNA diversity from a pre-Columbian population with contemporary Native American and Asian mtDNA lineages and to examine hypotheses about the peopling of the New World. Haplogroup and hypervariable region I sequence data indicate that the lineages from haplogroups A, B, C, and D are the most common among Native Americans but that they were not the only lineages brought into the New World from Asia. The mtDNA evidence does not support the three-wave hypothesis of migration into the New World but rather suggests a single "wave" of people with considerable mtDNA diversity that exhibits a signature of expansion 23,000-37,000 years ago.
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Whole mitogenome reconstruction from dental calculus was successful for the three individuals (NF-15, NF-217, and NF-262) that had previously failed to yield amplifiable mtDNA from bone.
“Importantly, whole mitogenome reconstruction from dental calculus was successful for the three individuals (NF‐15, NF‐217, and NF‐262) that had previously failed to yield amplifiable mtDNA from bone (Stone and Stoneking, 1998).”
From Successful enrichment and recovery of whole mitochondrial genomes from ancient human dental calculus (2016) · cites this paper as Stone and Stoneking, 1998
The Results report that 44 of 152 Norris Farms samples did not contain sufficient DNA for analysis, and Subjects and Methods says DNA was extracted primarily from bone. The paper does not identify NF-15, NF-217, or NF-262 as failed samples, so it cannot verify the failure attributed to those three individuals. The cited paper does not report the dental-calculus reconstruction, which appears to be the citing authors’ own finding. (LLM, conf=0.84)
Experimental — accuracy not yet measuredThe Norris Farms #36 cemetery was selected for analysis because bone samples from this site had been previously investigated using ancient DNA techniques, and mtDNA haplogroup assignments and HVRI sequences are available for 108 and 52 individuals, respectively, making it the most intensively studied pre-Contact North American site to date.
“The Norris Farms #36 cemetery was selected for analysis because bone samples from this site had been previously investigated using ancient DNA techniques (Stone and Stoneking, 1993, 1998, 1999), and mtDNA haplogroup assignments and HVRI sequences are available for 108 and 52 individuals, respectively, making it the most intensively studied pre‐Contact North American site to date.”
From Successful enrichment and recovery of whole mitochondrial genomes from ancient human dental calculus (2016) · cites this paper as Stone and Stoneking, 1998
Split into 2 atoms — 1 confirmed, 1 partially supported, 0 disputed, 0 misattributed, 0 unverifiable. Expand the claim to see per-atom verdicts.
Experimental — accuracy not yet measuredOf the six individuals, ribs from three (NF-15, NF-217, and NF-262) were previously unsuccessful for mtDNA haplogroup analysis and HVRI sequencing using targeted PCR, while three other rib samples (NF-47, NF-95, and NF-108) successfully produced results for mtDNA haplogroup markers, HVRI sequences, and amelogenin gene sex identification markers.
“Of these, ribs from three individuals (NF‐15, NF‐217, and NF‐262) were previously unsuccessful for mtDNA haplogroup analysis and HVRI sequencing using targeted PCR, while three other rib samples (NF‐47, NF‐95, and NF‐108) successfully produced results for mtDNA haplogroup markers, HVRI sequences, and amelogenin gene sex identification markers (Stone et al., 1996; Stone and Stoneking, 1998).”
From Successful enrichment and recovery of whole mitochondrial genomes from ancient human dental calculus (2016) · cites this paper as Stone and Stoneking, 1998
Split into 2 atoms — 0 confirmed, 2 partially supported, 0 disputed, 0 misattributed, 0 unverifiable. Expand the claim to see per-atom verdicts.
Experimental — accuracy not yet measuredHaplogroup assignments from dental calculus matched those for paired dentine samples in this study and matched bone samples previously analyzed by targeted PCR of the HVRI region.
“haplogroup assignments matched those for paired dentine samples in this study and matched bone samples previously analyzed by targeted PCR of the HVRI region (Stone and Stoneking, 1998) (Table 4).”
From Successful enrichment and recovery of whole mitochondrial genomes from ancient human dental calculus (2016) · cites this paper as Stone and Stoneking, 1998
Three of the six individuals from whom mitogenomes were reconstructed had previously tested negative for DNA preservation in bone using conventional PCR techniques.
“including three individuals who had previously tested negative for DNA preservation in bone using conventional PCR techniques”
From Successful enrichment and recovery of whole mitochondrial genomes from ancient human dental calculus (2016) · cites this paper as Stone and Stoneking, 1998
The Introduction describes an earlier PCR-based screening of 100 adults and says some were reexamined, but it does not identify three individuals who previously tested negative. The Results report mtDNA findings for a much larger sample, not six reconstructed mitogenomes. Searches for prior negative PCR tests and the three-of-six figure found no passage establishing the claim. (LLM, conf=0.74)
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References 87 · Cited by 287
Research works printed but not linked: 5
- Greenberg JH (1987) Language in the Americas. Stanford University Press, Stanford
- Handt O (1995) Molekulargenetische analyse alter menschlicher DNA Biology. PhD thesis, Ludwig-MaximilliansUniversitat, Munich
- Thornton R (1987) American Indian holocaust and survival: a population history since 1492. University of Oklahoma Press, Norman
- Vigilant L (1990) Control region sequences from African populations and the evolution of human mitochondrial DNA Biochemistry. PhD thesis, University of California (Berkeley), Berkeley
- Wallace DC, Torroni A (1992) American Indian prehistory as written in the mitchondrial DNA: a review. Hum Biol 64: 403-416
- Authors
- Stone, A. C., & Stoneking, M.
- Affiliations
- Pennsylvania State University (US)
- Corresponding author
- Stone, Anne C.
- Venue
- The American Journal of Human Genetics
- Publisher
- Elsevier BV
- Published
- 1998-05
- Volume
- 62
- Issue
- 5
- Pages
- 1153-1170
- ISSN
- 0002-9297 (ISSN-L)
- PMC
- PMC1377095
- OpenAlex
- W2033697698
- Cited by
- 287
- References
- 87
- Citations by year
- 2023: 2 · 2022: 2 · 2021: 5 · 2020: 3 · 2019: 2 · 2018: 4 · 2017: 2 · 2016: 9 · 2015: 2 · 2014: 5 · 2013: 8 · 2012: 6
- FWCI
- 11.65
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- The American Journal of Human Genetics (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Forensic and Genetic Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic diversity and population structure
- Social Sciences › Anthropology › Pleistocene-Era Hominins and Archaeology
- Keywords
- mitochondrial DNA, Oneota, prehistoric Native Americans, peopling of the Americas, mtDNA haplogroups, hypervariable region 1, migration waves, population growth
- MeSH terms
- DNA, Mitochondrial, Humans, Indians, North American, Mathematical Computing, Population
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