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Zinc‐Reversible Antimicrobial Activity of Recombinant Calprotectin (Migration Inhibitory Factor–Related Proteins 8 and 14)
Abstract
Recombinant calprotectin, consisting of 2 individual peptide chains also called migration inhibitory factor-related protein (MRP)-8 and MRP14, was tested for antimicrobial activity in a Candida albicans growth inhibition assay. Both chains contain HEXXH zinc-binding sites and might be expected to manifest zinc-reversible, antimicrobial activity similar to that of native calprotectin. When tested alone, neither MRP8 nor MRP14 showed activity in the Candida growth assay. A synthetic 20-amino acid peptide containing the HEXXH sequence of MRP14, along with a nearby HHH sequence, was also inactive in this assay. However, equimolar concentrations of MRP8 and MRP14 demonstrated a potent growth inhibitory effect that was reversible by 30 microM zinc. Truncated MRP14 (missing the C-terminal GHHHKPGLGEGTP tail) used in combination with MRP8 demonstrated zinc-reversible activity that was somewhat less than that with complete MRP14. These results suggest that intact calprotectin, consisting of a heterodimer of MRP8 and MRP14, is necessary to form a zinc-binding site capable of inhibiting microbial growth.
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Cited by
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Kehl-Fie, T. E., Chitayat, S., Hood, M. I., Damo, S., Restrepo, N., Garcia, C., Munro, K. A., Chazin, W. J., & Skaar, E. P. (2011) · Cell Host & Microbe · doi:10.1016/j.chom.2011.07.004 · Cited by 419
Self-citationDamo, S. M., Kehl-Fie, T. E., Sugitani, N., Holt, M. E., Rathi, S., Murphy, W. J., Zhang, Y., Betz, C., Hench, L., Fritz, G., et al. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1220341110 · Cited by 388
Self-citationLiu, J. Z., Jellbauer, S., Poe, A. J., Ton, V., Pesciaroli, M., Kehl-Fie, T. E., Restrepo, N. A., Hosking, M. P., Edwards, R. A., Battistoni, A., et al. (2012) · Cell Host & Microbe · doi:10.1016/j.chom.2012.01.017 · Cited by 328
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Metadata
- Authors
- Sohnle, P. G., Hunter, M. J., Hahn, B., & Chazin, W. J.
- Venue
- The Journal of Infectious Diseases
- Publisher
- Oxford University Press (OUP)
- Published
- 2000-10
- Volume
- 182
- Issue
- 4
- Pages
- 1272-1275
- ISSN
- 0022-1899 (ISSN-L)
- 1537-6613
- OpenAlex
- W2140816250
- Cited by
- 224
- References
- 0
- FWCI
- 1.35
- Type
- journal article
- Language
- en
- Open access
- closed
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- Sohnle, P. G., Hunter, M. J., Hahn, B., & Chazin, W. J. (2000). Zinc‐Reversible Antimicrobial Activity of Recombinant Calprotectin (Migration Inhibitory Factor–Related Proteins 8 and 14). The Journal of Infectious Diseases. https://doi.org/10.1086/315810
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