Cultivation in Vitro of Excised Pea Roots
Abstract
1. A medium suitable for the growth of excised pea roots has been described. This medium contains 4 per cent sucrose in addition to inorganic salts. 2. Freshly excised pea root tips 3-4 mm. long grow 60 to 95 mm. in this medium in the first week; 200 mm. or more in the first two weeks. 3. Yeast extract (0.01 per cent) must be added to the basic medium if good growth in passages after the first is to be obtained. Roots have been kept in culture with weekly transfers for four months on the basic medium with added yeast extract. 4. Crystalline vitamin B1 is capable of partially replacing yeast extract; vitamin B1 alone is not able to support the continued optimal growth of excised pea roots in later passages. 5. A mixture of pure, crystalline amino acids is capable of replacing that portion of the activity of yeast extract which is not due to vitamin B1. With the use of vitamin B1 and this mixture of amino acids a highly satisfactory, completely known medium for the cultivation of excised pea roots has been obtained. This medium of known composition supports the growth of such roots as well or better than media containing yeast extract. 6. Substances such as vitamin B1, which are necessary in minute amounts for the growth of the isolated root but which are normally supplied by some other portion of the intact plant, are carriers of a growth correlation and hence are to be regarded as phytohormones.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Such roots are very sensitive to differences in cultural conditions such as temperature, pH, osmotic value, concentration and proportion of nutrient salts, and carbohydrate supply; this sensitivity manifests itself either in change of growth habit or change in growth rate.
“Such roots are, in fact, very sensitive to differences in cultural conditions such as temperature, pH (White, 1932, 63), osmotic value (Bonner and Addicott, 1937, 42), concentra- tion and proportion of nutrient salts (White, 1933, 141, 1937, 143; Bonner and Addicott, 1937, 42), carbohydrate supply (White, 1932, 63, 1940, 172, 173), etc.; this sensitivity manifests itself either in change of growth”
From A handbook of plant tissue culture, by Philip R. White... (1943) · cites this paper as Bonner and Addicott, 1937
The only universally necessary member of the vitamin group seems to be thiamin (vitamin B1).
“The only universally necessary member of this group seems to be thiamin (vitamin B x ) (Bonner, 1937, 198, 199; Robbins and Bartley, 1937, 222, 223 ; White, 1937, 231 ; Gautheret, 1938, 69 ; Nobecourt, 1938, 75).”
From A handbook of plant tissue culture, by Philip R. White... (1943) · cites this paper as Bonner, 1937, 198, 199
In many types of tissues, the requirement for thiamin becomes evident in complete failure of the cultures if thiamin or thiazole is not included in the nutrient.
“In many types of tissues, this requirement becomes evident in com- plete failure of the cultures if thiamin or thiazole is not included in the nutrient (Bonner, 1937(Bonner, , 198, 1938, 199; Robbins and Bartley, 1937, 222, 223; White, 1937, 231, 1939, 182 ; Bonner and Devirian, 1939, 205).”
From A handbook of plant tissue culture, by Philip R. White... (1943) · cites this paper as Bonner, 1937, 198
Cited by
Sorted by citations
A handbook of plant tissue culture, by Philip R. White...
White, P. R. (1943) · doi:10.5962/bhl.title.4656 · Cited by 434 · see citing works
Höxtermann, E. (1997) · Physiologia Plantarum · doi:10.1111/j.1399-3054.1997.tb03079.x · Cited by 15 · see citing works
- Authors
- Bonner, J., & Addicott, F.
- Venue
- Botanical Gazette
- Publisher
- University of Chicago Press
- Published
- 1937-09
- Volume
- 99
- Issue
- 1
- Pages
- 144-170
- ISSN
- 0006-8071 (ISSN-L)
- OpenAlex
- W2025211487
- Cited by
- 130
- References
- 0
- Citations by year
- 2024: 1 · 2016: 1 · 2015: 1 · 2014: 2
- FWCI
- 15.94
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Botanical Gazette (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- Pisum sativum, in vitro root culture, culture medium composition, sucrose, yeast extract, thiamine, amino acids, plant hormones, root tip growth, plant tissue culture
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
