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Regulation of Staphylococcus aureus α‐Toxin Gene(hla)Expression by agr, sarA,and sae In Vitro and in Experimental Infective Endocarditis
Abstract
BACKGROUND: The Staphylococcus aureus global regulators--agr, sarA, and sae--coordinately control alpha-toxin gene (hla) expression in vitro. However, their relative in vivo contributions to hla expression, particularly in endovascular infections, have not been defined. METHODS: A plasmid-based hla-promoter:green fluorescent protein reporter system was constructed in 2 genetically related S. aureus strains: RN6390 (a natural sigma factor B [sigB]-deficient mutant), SH1000 (a sigB-repaired variant of RN6390 lineage), and their respective agr, sarA, agr/sarA, and sae mutants. These strain sets were used to quantify hla expression in vitro and in an experimental infective endocarditis (IE) model using flow cytometry. RESULTS: In vitro, hla expression was positively modulated by all 3 regulons (sae > agr/sarA > agr and sarA) in both RN6390 and SH1000 backgrounds. In the IE model, hla expression in cardiac vegetations was lower in all single mutants than in the respective parental strains (P<.05 for sae mutant) but was maintained at near-parental levels in the agr/sarA double mutant in both backgrounds. A similar finding was also observed in kidneys and spleens. CONCLUSIONS: These results indicate that, although several distinct regulatory circuits can affect hla expression in vitro and in vivo, sae appears to play a crucial role in this context.
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“SarA not only induces the transcription of ica operon but also of its suppressor icaR, indicating that SarA may prevent the excessive production of PIA [109].”
From A Review of Biofilm Formation of Staphylococcus aureus and Its Regulation Mechanism (2022) · cites this paper as [109]
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“SaeRS regulates the expression of genes associated with biofilm adhesion proteins such as fnbA, fnbB, and fib [109,145,146].”
From A Review of Biofilm Formation of Staphylococcus aureus and Its Regulation Mechanism (2022) · cites this paper as [109]
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Cited by
A Review of Biofilm Formation of Staphylococcus aureus and Its Regulation Mechanism
Peng, Q., Tang, X., Dong, W., Sun, N., & Yuan, W. (2022) · Antibiotics · doi:10.3390/antibiotics12010012 · Cited by 440
Kennedy, A. D., Wardenburg, J. B., Gardner, D. J., Long, D., Whitney, A. R., Braughton, K. R., Schneewind, O., & DeLeo, F. R. (2010) · The Journal of Infectious Diseases · doi:10.1086/656043 · Cited by 335
Fleitas Martínez, O., Cardoso, M. H., Ribeiro, S. M., & Franco, O. L. (2019) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2019.00074 · Cited by 304
Torres, V. J., Attia, A. S., Mason, W. J., Hood, M. I., Corbin, B. D., Beasley, F. C., Anderson, K. L., Stauff, D. L., McDonald, W. H., Zimmerman, L. J., et al. (2010) · Infection and Immunity · doi:10.1128/iai.01423-09 · Cited by 169
Eisen, D. P., Corey, G. R., McBryde, E. S., Fowler, V. G., Miro, J. M., Cabell, C. H., Street, A. C., Paiva, M. G., Ionac, A., Tan, R.-S., et al. (2009) · Journal of Infection · doi:10.1016/j.jinf.2009.03.006 · Cited by 40
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Metadata
- Authors
- Xiong, Y. Q., Willard, J., Yeaman, M. R., Cheung, A. L., & Bayer, A. S.
- Venue
- The Journal of Infectious Diseases
- Publisher
- Oxford University Press (OUP)
- Published
- 2006-11
- Volume
- 194
- Issue
- 9
- Pages
- 1267-1275
- ISSN
- 0022-1899 (ISSN-L)
- 1537-6613
- OpenAlex
- W2152862371
- Cited by
- 162
- References
- 0
- FWCI
- 3.44
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Regulation of Staphylococcus aureus α‐Toxin Gene(hla)Expression by agr, sarA,and sae In Vitro and in Experimental Infective Endocarditis. https://app.cochat.ai/papers/10.1086/508210
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- Xiong, Y. Q., Willard, J., Yeaman, M. R., Cheung, A. L., & Bayer, A. S. (2006). Regulation of Staphylococcus aureus α‐Toxin Gene(hla)Expression by agr, sarA,and sae In Vitro and in Experimental Infective Endocarditis. The Journal of Infectious Diseases. https://doi.org/10.1086/508210
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