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NanoJ: a high-performance open-source super-resolution microscopy toolbox
Open accessAbstract
Super-resolution microscopy (SRM) has become essential for the study of nanoscale biological processes. This type of imaging often requires the use of specialised image analysis tools to process a large volume of recorded data and extract quantitative information. In recent years, our team has built an open-source image analysis framework for SRM designed to combine high performance and ease of use. We named it NanoJ-a reference to the popular ImageJ software it was developed for. In this paper, we highlight the current capabilities of NanoJ for several essential processing steps: spatio-temporal alignment of raw data (NanoJ-Core), super-resolution image reconstruction (NanoJ-SRRF), image quality assessment (NanoJ-SQUIRREL), structural modelling (NanoJ-VirusMapper) and control of the sample environment (NanoJ-Fluidics). We expect to expand NanoJ in the future through the development of new tools designed to improve quantitative data analysis and measure the reliability of fluorescent microscopy studies.
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Cited by
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Metadata
- Authors
- Laine, R. F., Tosheva, K. L., Gustafsson, N., Gray, R. D. M., Almada, P., Albrecht, D., Risa, G. T., Hurtig, F., Lindås, A.-C., Baum, B., Mercer, J., Leterrier, C., Pereira, P. M., Culley, S., & Henriques, R.
- Venue
- Journal of Physics D: Applied Physics
- Publisher
- IOP Publishing
- Published
- 2019-02-18
- Volume
- 52
- Issue
- 16
- Pages
- 163001
- ISSN
- 0022-3727 (ISSN-L)
- 1361-6463
- OpenAlex
- W2951719549
- Cited by
- 188
- References
- 0
- FWCI
- 8.91
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Laine, R. F., Tosheva, K. L., Gustafsson, N., Gray, R. D. M., Almada, P., Albrecht, D., Risa, G. T., Hurtig, F., Lindås, A.-C., Baum, B., Mercer, J., Leterrier, C., Pereira, P. M., Culley, S., & Henriques, R. (2019). NanoJ: a high-performance open-source super-resolution microscopy toolbox. Journal of Physics D: Applied Physics. https://doi.org/10.1088/1361-6463/ab0261
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