The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
Optimization of conventional plant surface sterilization procedure for isolation of phosphate solubilizing endophytic bacteria from wresah and peanut leaves
Open accessAbstract
Endophytes are microorganisms that colonize and live within plant tissues. The success of endophytes isolation was determined by surface sterilization to remove contaminants. Many studies have reported root surface sterilization for isolation endophytic bacteria but there is still limited information about leaf surface sterilization. This study aims to optimize the effectiveness of conventional plant surface sterilization procedure for isolation of P-solubilizing endophytic bacteria from wresah and peanut leaves. The assessment was conducted in four procedures: Procedure A, the leaf samples were rinsed with only running water; Procedure B, the leaf samples were sterilized sequentially with 70% ethanol, 2.5% NaOCl and sterile distilled water; Procedure C and Procedure D were similar to Procedure B, but using the higher concentration of NaOCl (3.25 and 5.25%). For all procedures, 0.1 ml aliquot from the final rinse water, serial dilution of whole leaves and mashed leaves were inoculated on Pikovskaya media. The results showed as the concentration of NaOCl increased, the surface bacterial contamination of leaf samples decreased. Samples sterilized by Procedure B showed the highest endophytic bacterial population density of 0.014x10 5 cfug−1 (wresah leaves) and 0.072x10 5 cfug−1 (peanut leaves). Procedure B was found effective in removing contaminants and maintaining the endophytic bacteria community.
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IOP Conference Series: Earth and Environmental Science
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Surface sterilization for isolation of endophytes: Ensuring what (not) to grow
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Chemistry and bioactivity of plants from the genus Amomum
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Research works printed but not linked: 6
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- Anjum, 2015, Asian Journal of Pharmaceutical and Clinical Research, 8
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Metadata
- Authors
- Prasgi, H. C., Ariyanto, D. P., & Cahyani, V. R.
- Affiliations
- Sebelas Maret University (ID)
- Venue
- IOP Conference Series: Earth and Environmental Science
- Publisher
- IOP Publishing
- Published
- 2025-04-01
- Volume
- 1490
- Issue
- 1
- Pages
- 012025
- ISSN
- 1755-1315 (ISSN-L)
- 1755-1307
- OpenAlex
- W4409947224
- Semantic Scholar
- a6a9d14f9e42b3abf134cf537c4aaef34ce64e8c
- Cited by
- 2
- References
- 19
- Citations by year
- 2026: 2
- FWCI
- 4.77
- Type
- conference paper
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- Locations
- IOP Conference Series Earth and Environmental Science (publishedVersion, cc-by)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Plant and fungal interactions
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Agriculture, Plant Science, Crop Management
- Keywords
- endophytic bacteria, phosphate solubilization, groundnut, leaf endophytes, ethanol treatment, bacterial contamination removal, endophyte isolation
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