3D bioprinting of BM-MSCs-loaded ECM biomimetic hydrogels for in vitro neocartilage formation
Open accessAbstract
In this work we demonstrate how to print 3D biomimetic hydrogel scaffolds for cartilage tissue engineering with high cell density (>10 7 cells ml −1 ), high cell viability (85 ÷ 90%) and high printing resolution (≈100 μ m) through a two coaxial-needles system. The scaffolds were composed of modified biopolymers present in the extracellular matrix (ECM) of cartilage, namely gelatin methacrylamide (GelMA), chondroitin sulfate amino ethyl methacrylate (CS-AEMA) and hyaluronic acid methacrylate (HAMA). The polymers were used to prepare three photocurable bioinks with increasing degree of biomimicry: (i) GelMA, (ii) GelMA + CS-AEMA and (iii) GelMA + CS-AEMA + HAMA. Alginate was added to the bioinks as templating agent to form stable fibers during 3D printing. In all cases, bioink solutions were loaded with bone marrow-derived human mesenchymal stem cells (BM-MSCs). After printing, the samples were cultured in expansion (negative control) and chondrogenic media to evaluate the possible differentiating effect exerted by the biomimetic matrix or the synergistic effect of the matrix and chondrogenic supplements. After 7, 14, and 21 days, gene expression of the chondrogenic markers (COL2A1 and aggrecan), marker of osteogenesis (COL1A1) and marker of hypertrophy (COL10A1) were evaluated qualitatively by means of fluorescence immunocytochemistry and quantitatively by means of RT-qPCR. The observed enhanced viability and chondrogenic differentiation of BM-MSCs, as well as high robustness and accuracy of the employed deposition method, make the presented approach a valid candidate for advanced engineering of cartilage tissue.
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Various biofunctional materials and cell types can be printed simultaneously onto a substrate of cell-compatible biomaterials to build 3D complex constructs with good spatial resolution and reproducibility.
“Various biofunctional materials and cell types can thus be printed simultaneously onto a substrate of cell-compatible biomaterials to build 3D complex constructs with good spatial resolution and reproducibility (Fig. 3a)130,148–151.”
From Tumor-on-a-chip: from bioinspired design to biomedical application (2021) · cites this paper as [149]
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References 45 · Cited by 250
- Authors
- Costantini, M., Idaszek, J., Szöke, K., Jaroszewicz, J., Dentini, M., Barbetta, A., Brinchmann, J. E., & Święszkowski, W.
- Affiliations
- Warsaw University of Technology (PL)
- Oslo University Hospital (NO)
- Sapienza University of Rome (IT)
- ORCID
- Venue
- Biofabrication
- Publisher
- IOP Publishing
- Published
- 2016-07-18
- Volume
- 8
- Issue
- 3
- Pages
- 035002
- ISSN
- 1758-5082 (ISSN-L)
- 1758-5090
- OpenAlex
- W2478743684
- Cited by
- 250
- References
- 45
- Citations by year
- 2026: 8 · 2025: 15 · 2024: 17 · 2023: 20 · 2022: 34 · 2021: 35 · 2020: 36 · 2019: 29 · 2018: 31 · 2017: 23 · 2016: 1 · 2012: 1
- FWCI
- 13.50
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Biofabrication (publishedVersion)
- PubMed (publishedVersion)
- IRIS Research product catalog (Sapienza University of Rome) (submittedVersion)
- Funders
- Ateneo Found - La Sapienza
- NewJoint (Nor/202132/68/2013)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- 3D bioprinting, cartilage tissue engineering, bone marrow-derived mesenchymal stem cells, biomimetic hydrogels, gelatin methacryloyl, hyaluronic acid methacrylate, chondrogenic differentiation, neocartilage formation, COL2A1, aggrecan, COL10A1
- MeSH terms
- Bone Marrow Cells, Collagen, Extracellular Matrix, Humans, Hydrogels, Biomimetic Materials, Aggrecans, Mesenchymal Stem Cells, Printing, Three-Dimensional
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