The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.
DNA Source Selection for Downstream Applications Based on DNA Quality Indicators Analysis
Open accessAbstract
High-quality human DNA samples and associated information of individuals are necessary for biomedical research. Biobanks act as a support infrastructure for the scientific community by providing a large number of high-quality biological samples for specific downstream applications. For this purpose, biobank methods for sample preparation must ensure the usefulness and long-term functionality of the products obtained. Quality indicators are the tool to measure these parameters, the purity and integrity determination being those specifically used for DNA. This study analyzes the quality indicators in DNA samples derived from 118 frozen human tissues in optimal cutting temperature (OCT) reactive, 68 formalin-fixed paraffin-embedded (FFPE) tissues, 119 frozen blood samples, and 26 saliva samples. The results obtained for DNA quality are discussed in association with the usefulness for downstream applications and availability of the DNA source in the target study. In brief, if any material is valid, blood is the most approachable option of prospective collection of samples providing high-quality DNA. However, if diseased tissue is a requisite or samples are available, the recommended source of DNA would be frozen tissue. These conclusions will determine the best source of DNA, according to the planned downstream application. Furthermore our results support the conclusion that a complete procedure of DNA quantification and qualification is necessary to guarantee the appropriate management of the samples, avoiding low confidence results, high costs, and a waste of samples.
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Cites
Singer, V. L., Jones, L. J., Yue, S. T., & Haugland, R. P. (1997) · Analytical Biochemistry · doi:10.1006/abio.1997.2177 · Cited by 778
Effect of pH and Ionic Strength on the Spectrophotometric Assessment of Nucleic Acid Purity
Wilfinger, W. W., Mackey, K., & Chomczynski, P. (1997) · BioTechniques · doi:10.2144/97223st01 · Cited by 553
Wagle, N., Berger, M. F., Davis, M. J., Blumenstiel, B., DeFelice, M., Pochanard, P., Ducar, M., Van Hummelen, P., MacConaill, L. E., Hahn, W. C., et al. (2012) · Cancer Discovery · doi:10.1158/2159-8290.cd-11-0184 · Cited by 533
The Condensed Protocols from Molecular Cloning: A Laboratory Manual
Russell, D. W., Sambrook, J., & Russell, D. W. (2006) · Medical Entomology and Zoology · Cited by 526
Validity of nucleic acid purities monitored by 260nm/280nm absorbance ratios.
Glasel, J. A. (1995) · PubMed · Cited by 436
Collection of genomic DNA from adults in epidemiological studies by buccal cytobrush and mouthwash.
García‐Closas, M., Egan, K. M., Abruzzo, J., Newcomb, P. A., Titus-Ernstoff, L. T., Franklin, T., Bender, P. K., Beck, J. C., Marchand, L. L., Lum, A., et al. (2001) · PubMed · Cited by 285
DNA Qualification Workflow for Next Generation Sequencing of Histopathological Samples
Simbolo, M., Gottardi, M., Corbo, V., Fassan, M., Mafficini, A., Malpeli, G., Lawlor, R. T., & Scarpa, A. (2013) · PLoS ONE · doi:10.1371/journal.pone.0062692 · Cited by 282
Evaluation of saliva as a source of human DNA for population and association studies
Quinque, D., Kittler, R., Kayser, M., Stoneking, M., & Nasidze, I. (2006) · Analytical Biochemistry · doi:10.1016/j.ab.2006.03.021 · Cited by 191
Revisiting absorbance at 230 nm as a protein unfolding probe
Liu, P.-F., Avramova, L. V., & Park, C. (2009) · Analytical Biochemistry · doi:10.1016/j.ab.2009.03.028 · Cited by 84
Robustness of genome-wide scanning using archived dried blood spot samples as a DNA source
Hollegaard, M. V., Grove, J., Grauholm, J., Kreiner-Møller, E., Bønnelykke, K., Nørgaard, M., Benfield, T. L., Nørgaard-Pedersen, B., Mortensen, P. B., Mors, O., et al. (2011) · BMC Genetics · doi:10.1186/1471-2156-12-58 · Cited by 83
Cited by
Naveed, M., Tianying, H., Wang, F., Yin, X., Chan, M. W. H., Ullah, A., Xu, B., Aslam, S., Ali, N., Abbas, Q., et al. (2022) · Current Research in Biotechnology · doi:10.1016/j.crbiot.2022.06.002 · Cited by 26
Metadata
- Authors
- Lucena-Aguilar, G., Sánchez-López, A. M., Barberán-Aceituno, C., Carrillo-Ávila, J. A., López-Guerrero, J. A., & Aguilar-Quesada, R.
- Venue
- Biopreservation and Biobanking
- Publisher
- SAGE Publications
- Published
- 2016-08
- Volume
- 14
- Issue
- 4
- Pages
- 264-270
- ISSN
- 1947-5535 (ISSN-L)
- 1947-5543
- OpenAlex
- W2415930568
- Cited by
- 286
- References
- 16
- FWCI
- 2.56
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Lucena-Aguilar, G., Sánchez-López, A. M., Barberán-Aceituno, C., Carrillo-Ávila, J. A., López-Guerrero, J. A., & Aguilar-Quesada, R. (2016). DNA Source Selection for Downstream Applications Based on DNA Quality Indicators Analysis. Biopreservation and Biobanking. https://doi.org/10.1089/bio.2015.0064
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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