Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 46Pineapple and bromelain studiesImmune Response and InflammationNeutrophil, Myeloperoxidase and Oxidative Mechanisms

The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.

Proteolytic Enzymes and Amylase Induce Cytokine Production in Human Peripheral Blood Mononuclear Cells in vitro

Desser, Lucia, Rehberger, Alexander, Paukovits, Walter·1994·Cancer Biotherapy
DOI 10.1089/cbr.1994.9.253
Open this paper in CoChat — free account

Abstract

In vitro treatment of human peripheral blood mononuclear cells (PBMNC) with proteolytic enzymes (bromelain, papain) and amylase leads to the production of large amounts of tumor necrosis factor-alpha (TNF-alpha), interleukin-1-beta (IL-1 beta), and interleukin-6 (IL-6) in a time and dose dependent manner. Increased TNF-alpha and IL-6 production was already found after 4-6 hours of incubation, and plateau levels were reached after 12-16 hours. Plateau levels up to 1500 pg TNF-alpha/ml/10(6) PBMNC, 13000 pg IL-1 beta/ml/10(6) PBMNC, and 23000 pg IL-6/ml/10(6) PBMNC were observed. Control cultures contained below 35 pg/ml/10(6) PBMNC of TNF-alpha, IL-1 beta or IL-6. In contrast to TNF-alpha which was undetectable after more than 24 hours, peak levels of IL-1 beta and IL-6 were still present at 24 hours. After incubation of the enzyme solution for some hours at 56 degrees C the cytokine inducing capacity disappeared. Neutralization experiments with inactivating antibodies, radioimmunoassay, and western blotting after electrophoretic separation showed that the TNF-like activity found in the lytic assay was due to TNF-alpha. Interferon-alpha (IFN-alpha) and Interferon-gamma (IFN-gamma), which had no effect alone, synergistically increased TNF-alpha production when applied together with the enzymes. A commercial mixture of these enzymes (Wobenzym), which was also investigated, showed a similar concentration and time dependence, as well as synergism with the interferons. A synergisti…

Overview

Integrity signals

Registry

Crossref · 10.1089/cbr.1994.9.253

Cites

12 of 12 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Research works printed but not linked: 1

  1. 1976, Chemotherapy, 8, 517

Cited by

See all 46

Related

See all

Metadata

Authors
Desser, L., Rehberger, A., & Paukovits, W.
Affiliations
University of Vienna (AT)
Venue
Cancer Biotherapy
Publisher
SAGE Publications
Published
1994-01
Volume
9
Issue
3
Pages
253-263
ISSN
1062-8401 (ISSN-L)
DOI
10.1089/cbr.1994.9.253
OpenAlex
W2091127196
Cited by
45
References
12
Citations by year
2025: 2 · 2023: 2 · 2022: 1 · 2021: 3 · 2019: 2 · 2018: 2 · 2017: 2 · 2016: 4 · 2015: 2 · 2014: 2 · 2013: 1 · 2012: 2
FWCI
0.50
Type
journal article
Language
en
Open access
closed
Licence
www.liebertpub.com/nv/resources-tools/text-and-data-mining-policy/121/ (tdm)
Locations
  • Cancer Biotherapy (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pineapple and bromelain studies
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
  • Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Keywords
proteolytic enzymes, bromelain, papain, amylase, peripheral blood mononuclear cells, cytokine production, TNF-α, IL-1β, interleukin-6, interferon-alpha, interferon-gamma, enzyme synergy, phorbol ester, Wobenzym
MeSH terms
Amylases, Bromelains, Cells, Cultured, Drug Synergism, Gene Expression Regulation, Humans, Interferons, Interleukin-1, Leukocytes, Mononuclear, Papain, Tetradecanoylphorbol Acetate, Tumor Necrosis Factor-alpha, Interleukin-6, Cytokines

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.