The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Supercoil Sequencing: A Fast and Simple Method for Sequencing Plasmid DNA
Abstract
A method for obtaining sequence information directly from plasmid DNA is presented. The procedure involves the rapid preparation of clean supercoiled plasmid DNA from small bacterial cultures, its complete denaturation by alkali, and sequence determination using oligodeoxyribonucleotide-primed enzymatic DNA synthesis in the presence of dideoxynucleoside triphosphates. The advantages of the method include speed, simplicity, avoidance of additional cloning steps into single-stranded phage M13 vectors, and hence applicability to sequencing large numbers of samples.
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Citations
Cites
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
Cited by
floricaula: A homeotic gene required for flower development in antirrhinum majus
Coen, E. S., Romero, J., Doyle, S., Elliott, R., Murphy, G., & Carpenter, R. (1990) · Cell · doi:10.1016/0092-8674(90)90426-f · Cited by 835
Sorger, P. K., & Pelham, H. R. B. (1988) · Cell · doi:10.1016/s0092-8674(88)91219-6 · Cited by 832
The three operators of the lac operon cooperate in repression.
Oehler, S., Eismann, E. R., Krämer, H., & Müller-Hill, B. (1990) · The EMBO Journal · doi:10.1002/j.1460-2075.1990.tb08199.x · Cited by 473
Cloning and expression of rat cDNA encoding corticosteroid 11β-dehydrogenase
Agarwal, A. K., Monder, C., Eckstein, B., & White, P. C. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)47248-7 · Cited by 455
Tannin, G. M., Agarwal, A. K., Monder, C., New, M. I., & White, P. C. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55351-5 · Cited by 436
Metadata
- Authors
- CHEN, E. Y., & SEEBURG, P. H.
- Venue
- DNA
- Publisher
- SAGE Publications
- Published
- 1985-04
- Volume
- 4
- Issue
- 2
- Pages
- 165-170
- ISSN
- 0198-0238 (ISSN-L)
- OpenAlex
- W2025450446
- Cited by
- 2,436
- References
- 1
- FWCI
- 92.78
- Type
- journal article
- Language
- en
- Open access
- closed
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- CHEN, E. Y., & SEEBURG, P. H. (1985). Supercoil Sequencing: A Fast and Simple Method for Sequencing Plasmid DNA. DNA. https://doi.org/10.1089/dna.1985.4.165
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
