The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
βig-h3: A Transforming Growth Factor-β-Responsive Gene Encoding a Secreted Protein That Inhibits Cell Attachment In Vitro and Suppresses the Growth of CHO Cells in Nude Mice
Abstract
beta ig-h3 is a novel gene first discovered by differential screening of a cDNA library made from A549 human lung adenocarcinoma cells treated with transforming growth factor-beta 1 (TGF-beta 1). It encodes a 683-amino-acid protein containing a secretory signal sequence and four homologous internal domains. Here we show that treatment of several types of cells, including human melanoma cells, human mammary epithelial cells, human keratinocytes, and human fibroblasts, with TGF-beta resulted in a significant increase in beta ig-h3 RNA. A portion of the beta ig-h3 coding sequence was expressed in bacteria, and antisera against the bacterially produced protein was raised in rabbits. This antisera was used to demonstrate that several cell lines secreted a 68-kD beta IG-H3 protein after treatment with TGF-beta. Transfection of beta IG-H3 expression plasmids into Chinese hamster ovary (CHO) cells led to a marked decrease in the ability of these cells to form tumors in nude mice. The beta IG-H3 protein was purified from media conditioned by recombinant CHO cells, characterized by immunoblotting and protein sequencing and shown to function in an anti-adhesion assay in that it inhibited the attachment of A549, HeLa, and WI-38 cells to plastic in serum-free media. Sequencing of cDNA clones encoding murine beta ig-H3 indicated 90.6% conservation at the amino acid level between the murine and human proteins. Finally, the beta ig-h3 gene was localized to human chromosome 5q31, a region frequently deleted in preleukemic myelodysplasia and leukemia. The corresponding mouse beta ig-h3 gene was mapped to mouse chromosome 13 region B to C1, which confirms a region of conservation on human chromosome 5 and mouse chromosome 13. We suggest that this protein be named p68 beta ig-h3.
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Ignotz, R. A., & Massagué, J. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)35666-1 · Cited by 2,492
Identification of a Gene Family Regulated by Transforming Growth Factor-β
Brunner, A., Chinn, J., Neubauer, M., & Purchio, A. F. (1991) · DNA and Cell Biology · doi:10.1089/dna.1991.10.293 · Cited by 124
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Advances in animal cell recombinant protein production: GS-NS0 expression system
Barnes, L. M., Bentley, C. M., & Dickson, A. J. (2000) · Cytotechnology · doi:10.1023/a:1008170710003 · Cited by 129
Reinboth, B., Thomas, J., Hanssen, E., & Gibson, M. A. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m511316200 · Cited by 89
Metadata
- Authors
- SKONIER, J., BENNETT, K., ROTHWELL, V., KOSOWSKI, S., PLOWMAN, G., WALLACE, P., EDELHOFF, S., DISTECHE, C., NEUBAUER, M., MARQUARDT, H., RODGERS, J., & PURCHIO, A. F.
- Venue
- DNA and Cell Biology
- Publisher
- SAGE Publications
- Published
- 1994-06
- Volume
- 13
- Issue
- 6
- Pages
- 571-584
- ISSN
- 1044-5498 (ISSN-L)
- 1557-7430
- OpenAlex
- W1983773767
- Cited by
- 273
- References
- 2
- FWCI
- 1.35
- Type
- journal article
- Language
- en
- Open access
- closed
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- SKONIER, J., BENNETT, K., ROTHWELL, V., KOSOWSKI, S., PLOWMAN, G., WALLACE, P., EDELHOFF, S., DISTECHE, C., NEUBAUER, M., MARQUARDT, H., RODGERS, J., & PURCHIO, A. F. (1994). βig-h3: A Transforming Growth Factor-β-Responsive Gene Encoding a Secreted Protein That Inhibits Cell Attachment In Vitro and Suppresses the Growth of CHO Cells in Nude Mice. DNA and Cell Biology. https://doi.org/10.1089/dna.1994.13.571
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