The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Osteogenic Performance of Donor-Matched Human Adipose and Bone Marrow Mesenchymal Cells Under Dynamic Culture
Open accessAbstract
Adipose-derived mesenchymal cells (ACs) and bone marrow-derived mesenchymal cells (BMCs) have been widely used for bone regeneration and can be seeded on a variety of rigid scaffolds. However, to date, a direct comparison of mesenchymal cells (MC) harvested from different tissues from the same donor and cultured in identical osteogenic conditions has not been investigated. Indeed, it is unclear whether marrow-derived or fat-derived MC possess intrinsic differences in bone-forming capabilities, since within-patient comparisons have not been previously done. This study aims at comparing ACs and BMCs from three donors ranging in age from neonatal to adult. Matched cells from each donor were studied in three distinct bioreactor settings, to determine the best method to create a viable osseous engineered construct. Human ACs and BMCs were isolated from each donor, cultured, and seeded on decellularized porcine bone (DCB) constructs. The constructs were then subjected to either static or dynamic (stirring or perfusion) bioreactor culture conditions for 7-21 days. Afterward, the constructs were analyzed for cell adhesion and distribution and osteogenic differentiation. ACs demonstrated higher seeding efficiency than BMCs. However, static and dynamic culture significantly increased BMCs proliferation more than ACs. In all conditions, BMCs demonstrated stronger osteogenic activity as compared with ACs, through higher alkaline phosphatase activity and gene expression for various bony markers. Conversely, ACs expressed more collagen I, which is a nonspecific matrix molecule in most connective tissues. Overall, dynamic bioreactor culture conditions enhanced osteogenic gene expression in both ACs and BMCs. Scaffolds seeded with BMCs in dynamic stirring culture conditions exhibit the greatest osteogenic proliferation and function in vitro, proving that marrow-derived MC have superior bone-forming potential as compared with adipose-derived cells.
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“Regarding bone tissue engineering, studies comparing the osteogenic potential of human BM-MSCs and ASCs have yielded mixed results.”
From Mesenchymal Stem/Stromal Cells: Immunomodulatory and Bone Regeneration Potential after Tumor Excision in Osteosarcoma Patients (2023) · cites this paper as [60]
In the citing paper
“Some suggest that BM-MSCs exhibit greater osteogenic capacity [58,59,60,61,62], while others propose that ASCs have equal or superior potential [58,63,64,65,66].”
From Mesenchymal Stem/Stromal Cells: Immunomodulatory and Bone Regeneration Potential after Tumor Excision in Osteosarcoma Patients (2023) · cites this paper as [60]
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Cites
Stöckl, S., Göttl, C., Grifka, J. K., & Grässel, S. (2013) · Cellular Physiology and Biochemistry · doi:10.1159/000350089 · Cited by 4,670
Growth Factors, Matrices, and Forces Combine and Control Stem Cells
Discher, D. E., Mooney, D. J., & Zandstra, P. W. (2009) · Science · doi:10.1126/science.1171643 · Cited by 2,666
Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells
Rehman, J., Traktuev, D., Li, J., Merfeld-Clauss, S., Temm-Grove, C. J., Bovenkerk, J. E., Pell, C. L., Johnstone, B. H., Considine, R. V., & March, K. L. (2004) · Circulation · doi:10.1161/01.cir.0000121425.42966.f1 · Cited by 2,269
Recent advances in bone tissue engineering scaffolds
Bose, S., Roy, M., & Bandyopadhyay, A. K. (2012) · Trends in biotechnology · doi:10.1016/j.tibtech.2012.07.005 · Cited by 2,217
Park, D., Spencer, J. A., Koh, B. I., Kobayashi, T., Fujisaki, J., Clemens, T. L., Lin, C. P., Kronenberg, H. M., & Scadden, D. T. (2012) · Cell stem cell · doi:10.1016/j.stem.2012.02.003 · Cited by 637
Bone recognition mechanism of porcine osteocalcin from crystal structure
Hoang, Q. Q., Sicheri, F., Howard, A. J., & Yang, D. S. C. (2003) · Nature · doi:10.1038/nature02079 · Cited by 554
Engineering anatomically shaped human bone grafts
Grayson, W. L., Fröhlich, M., Yeager, K. J., Bhumiratana, S., Chan, M. E., Cannizzaro, C., Wan, L. Q., Liu, X. S., Guo, X.-D. E., & Vunjak‐Novakovic, G. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0905439106 · Cited by 425
Fluid and Solute Transport in Bone: Flow-Induced Mechanotransduction
Fritton, S. P., & Weinbaum, S. (2008) · Annual Review of Fluid Mechanics · doi:10.1146/annurev.fluid.010908.165136 · Cited by 393
Shear Stress Induces Osteogenic Differentiation of Human Mesenchymal Stem Cells
Yourek, G., McCormick, S. M., Mao, J. J., & Reilly, G. C. (2010) · Regenerative Medicine · doi:10.2217/rme.10.60 · Cited by 304
Yu, X., Botchwey, E. A., Levine, E. M., Pollack, S. R., & Laurencin, C. T. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0402532101 · Cited by 296
Cited by
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Adipose-derived and bone marrow mesenchymal stem cells: A donor-matched comparison
Mohamed-Ahmed, S., Fristad, I., Lie, S. A., Suliman, S., Mustafa, K., Vindenes, H., & Idris, S. B. (2018) · Stem Cell Research & Therapy · doi:10.1186/s13287-018-0914-1 · Cited by 515
Adipose Tissue-Derived Stem Cells in Regenerative Medicine
Frese, L., Dijkman, P. E., & Hoerstrup, S. P. (2016) · Transfusion Medicine and Hemotherapy · doi:10.1159/000448180 · Cited by 426
Ravichandran, A., Liu, Y., & Teoh, S. (2017) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.2270 · Cited by 68
Mohamed‐Ahmed, S., Yassin, M. A., Rashad, A., Espedal, H., Idris, S. B., Finne‐Wistrand, A., Mustafa, K. B. E., Vindenes, H. A., & Fristad, I. (2020) · Cell and Tissue Research · doi:10.1007/s00441-020-03315-5 · Cited by 67
Biophysical Stimuli as the Fourth Pillar of Bone Tissue Engineering
Hao, Z., Xu, Z., Wang, X., Wang, Y., Li, H., Chen, T., Hu, Y., Chen, R., Huang, K., Chen, C., et al. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.790050 · Cited by 67
Metadata
- Authors
- Wu, W., Le, A. V., Mendez, J. J., Chang, J., Niklason, L. E., & Steinbacher, D. M.
- Affiliations
- Yale University (US)
- Corresponding author
- Steinbacher, Derek M.
- Venue
- Tissue Engineering Part A
- Publisher
- SAGE Publications
- Published
- 2015-05
- Volume
- 21
- Issue
- 9-10
- Pages
- 1621-1632
- ISSN
- 1937-3341 (ISSN-L)
- 1937-335X
- PMC
- PMC4426327
- OpenAlex
- W1717550013
- Cited by
- 44
- References
- 29
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2023: 5 · 2022: 4 · 2021: 3 · 2020: 6 · 2019: 7 · 2018: 7 · 2017: 5 · 2016: 3
- FWCI
- 2.49
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Tissue Engineering Part A (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01eb008388-05)
- National Institute of General Medical Sciences (T32GM086287)
- National Institute of Biomedical Imaging and Bioengineering (R01 EB008388-03S1)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- osteogenic differentiation, bone regeneration, cell seeding efficiency, alkaline phosphatase activity, bone-specific gene expression, type I collagen, cell proliferation
- MeSH terms
- Adipose Tissue, Adult, Alkaline Phosphatase, Animals, Bone and Bones, Bone Marrow Cells, Calcium, Cell Adhesion, Cells, Cultured, Child, DNA, Female, Fluorescent Antibody Technique, Gene Expression Regulation, Humans, Infant, Male, Osteogenesis, Tissue Donors, Osteocalcin, Cell Culture Techniques, Bioreactors, Sus scrofa, Mesenchymal Stem Cells
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- CoChat. (2026). Citation check: Osteogenic Performance of Donor-Matched Human Adipose and Bone Marrow Mesenchymal Cells Under Dynamic Culture. https://app.cochat.ai/papers/10.1089/ten.tea.2014.0115
- The paper
- Wu, W., Le, A. V., Mendez, J. J., Chang, J., Niklason, L. E., & Steinbacher, D. M. (2015). Osteogenic Performance of Donor-Matched Human Adipose and Bone Marrow Mesenchymal Cells Under Dynamic Culture. Tissue Engineering Part A. https://doi.org/10.1089/ten.tea.2014.0115
