The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Evidence That Ternary Complex (eIF2-GTP-tRNAiMet)–Deficient Preinitiation Complexes Are Core Constituents of Mammalian Stress Granules
Open accessAbstract
Environmental stress-induced phosphorylation of eIF2α inhibits protein translation by reducing the availability of eIF2-GTP-tRNA i Met, the ternary complex that joins initiator tRNA Met to the 43S preinitiation complex. The resulting untranslated mRNA is dynamically routed to discrete cytoplasmic foci known as stress granules (SGs), a process requiring the related RNA-binding proteins TIA-1 and TIAR. SGs appear to be in equilibrium with polysomes, but the nature of this relationship is obscure. We now show that most components of the 48S preinitiation complex (i.e., small, but not large, ribosomal subunits, eIF3, eIF4E, eIF4G) are coordinately recruited to SGs in arsenite-stressed cells. In contrast, eIF2 is not a component of newly assembled SGs. Cells expressing a phosphomimetic mutant (S51D) of eIF2α assemble SGs of similar composition, confirming that the recruitment of these factors is a direct consequence of blocked translational initiation and not due to other effects of arsenite. Surprisingly, phospho-eIF2α is recruited to SGs that are disassembling in cells recovering from arsenite-induced stress. We discuss these results in the context of a translational checkpoint model wherein TIA and eIF2 are functional antagonists of translational initiation, and in which lack of ternary complex drives SG assembly.
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Cites
Regulated Translation Initiation Controls Stress-Induced Gene Expression in Mammalian Cells
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Self-citationDynamic Shuttling of Tia-1 Accompanies the Recruitment of mRNA to Mammalian Stress Granules
Kedersha, N., Cho, M. R., Li, W., Yacono, P. W., Chen, S., Gilks, N., Golan, D. E., & Anderson, P. (2000) · The Journal of Cell Biology · doi:10.1083/jcb.151.6.1257 · Cited by 814
Self-citationPhosphorylation of initiation factor eIF-2 and the control of reticulocyte protein synthesis
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TIA-1 is a translational silencer that selectively regulates the expression of TNF-α
Piecyk, M., Wax, S., Beck, A. R. P., Kedersha, N., Gupta, M., Maritim, B., Chen, S., Gueydan, C., Kruys, V., Streuli, M., et al. (2000) · The EMBO Journal · doi:10.1093/emboj/19.15.4154 · Cited by 509
Self-citationCONTROL OF TRANSLATION INITIATION IN ANIMALS
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Cited by
Stress granules: the Tao of RNA triage
Anderson, P., & Kedersha, N. (2008) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2007.12.003 · Cited by 1,116
Self-citationAnderson, P., & Kedersha, N. (2006) · The Journal of Cell Biology · doi:10.1083/jcb.200512082 · Cited by 984
Self-citationRETRACTED: The RasGAP-associated endoribonuclease G3BP assembles stress granules
Tourrière, H., Chebli, K., Zekri, L., Courselaud, B., Blanchard, J. M., Bertrand, E., & Tazi, J. (2003) · The Journal of Cell Biology · doi:10.1083/jcb.200212128 · Cited by 834
RetractedStress granules and neurodegeneration
Wolozin, B., & Ivanov, P. (2019) · Nature Reviews Neuroscience · doi:10.1038/s41583-019-0222-5 · Cited by 810
P-Body Purification Reveals the Condensation of Repressed mRNA Regulons
Hubstenberger, A., Courel, M., Bénard, M., Souquere, S., Ernoult-Lange, M., Chouaib, R., Yi, Z., Morlot, J.-B., Munier, A., Fradet, M., et al. (2017) · Molecular Cell · doi:10.1016/j.molcel.2017.09.003 · Cited by 787
Metadata
- Authors
- Kedersha, N., Chen, S., Gilks, N., Li, W., Miller, I. J., Stahl, J., & Anderson, P.
- Venue
- Molecular Biology of the Cell
- Published
- 2002-01
- Volume
- 13
- Issue
- 1
- Pages
- 195-210
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- OpenAlex
- W2049311171
- Cited by
- 622
- References
- 25
- FWCI
- 4.64
- Type
- journal article
- Language
- en
- Open access
- green
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- Kedersha, N., Chen, S., Gilks, N., Li, W., Miller, I. J., Stahl, J., & Anderson, P. (2002). Evidence That Ternary Complex (eIF2-GTP-tRNAiMet)–Deficient Preinitiation Complexes Are Core Constituents of Mammalian Stress Granules. Molecular Biology of the Cell. https://doi.org/10.1091/mbc.01-05-0221
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