Ammonia pulses and metabolic oscillations guide yeast colony development.
Open accessAbstract
On solid substrate, growing yeast colonies alternately acidify and alkalinize the medium. Using morphological, cytochemical, genetic, and DNA microarray approaches, we characterized six temporal steps in the "acid-to-alkali" colony transition. This transition is connected with the production of volatile ammonia acting as starvation signal between colonies. We present evidence that the three membrane proteins Ato1p, Ato2p, and Ato3p, members of the YaaH family, are involved in ammonia production in Saccharomyces cerevisiae colonies. The acid-to-alkali transition is connected with decrease of mitochondrial oxidative catabolism and by peroxisome activation, which in parallel with activation of biosynthetic pathways contribute to decrease the general stress level in colonies. These metabolic features characterize a novel survival strategy used by yeast under starvation conditions prevalent in nature.
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In conjunction with its two additional homologues, ATO2 and ATO3, ADY2 has also been implicated in ammonia export.
“In conjunction with its two additional homologues, ATO2 and ATO3, it has also been implicated in ammonia export (Palkova et al., 2002; Vachova et al., 2009).”
From Candida glabrata susceptibility to antifungals and phagocytosis is modulated by acetate (2015) · cites this paper as Palkova et al., 2002
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References 32 · Cited by 151
- Authors
- Palková, Z., Devaux, F., R̆ic̆icová, M., Mináriková, L., Le Crom, S., & Jacq, C.
- Affiliations
- Charles University (CZ)
- Centre National de la Recherche Scientifique (FR)
- Department of Genetics and Microbiology, Charles University, Vinic̆ná 5, 12844 Prague 2, Czech Republic; and
- Laboratoire de Génétique Moléculaire, CNRS 8541, Ecole Normale Supérieure, 75005 Paris, France
- Venue
- Molecular Biology of the Cell
- Published
- 2002-11
- Volume
- 13
- Issue
- 11
- Pages
- 3901-3914
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- PMC
- PMC133602
- OpenAlex
- W2150910409
- Cited by
- 151
- References
- 32
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 3 · 2023: 3 · 2022: 1 · 2021: 6 · 2020: 5 · 2019: 5 · 2018: 7 · 2017: 3 · 2016: 7 · 2015: 10 · 2014: 7 · 2013: 8 · 2012: 13
- FWCI
- 2.99
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Molecular Biology of the Cell (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Polyamine Metabolism and Applications
- Keywords
- metabolic oscillations, stress response, Saccharomyces cerevisiae
- MeSH terms
- Amino Acid Sequence, Amino Acids, Ammonia, Energy Metabolism, Fatty Acids, Hydrogen-Ion Concentration, Models, Biological, Molecular Sequence Data, Oxidative Phosphorylation, Phylogeny, Saccharomyces cerevisiae, Gene Expression Regulation, Fungal, Sequence Alignment, Oligonucleotide Array Sequence Analysis, Peroxisomes, Gene Expression Profiling, Membrane Transport Proteins, Saccharomyces cerevisiae Proteins
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