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Human PIG-U and Yeast Cdc91p Are the Fifth Subunit of GPI Transamidase That Attaches GPI-Anchors to Proteins
Open accessAbstract
Many eukaryotic proteins are anchored to the cell surface via glycosylphosphatidylinositol (GPI), which is posttranslationally attached to the carboxyl-terminus by GPI transamidase. The mammalian GPI transamidase is a complex of at least four subunits, GPI8, GAA1, PIG-S, and PIG-T. Here, we report Chinese hamster ovary cells representing a new complementation group of GPI-anchored protein-deficient mutants, class U. The class U cells accumulated mature and immature GPI and did not have in vitro GPI transamidase activity. We cloned the gene responsible, termed PIG-U, that encoded a 435-amino-acid hydrophobic protein. The GPI transamidase complex affinity-purified from cells expressing epitope-tagged-GPI8 contained PIG-U and four other known components. Cells lacking PIG-U formed complexes of the four other components normally but had no ability to cleave the GPI attachment signal peptide. Saccharomyces cerevisiae Cdc91p, with 28% amino acid identity to PIG-U, partially restored GPI-anchored proteins on the surface of class U cells. PIG-U and Cdc91p have a functionally important short region with similarity to a region conserved in long-chain fatty acid elongases. Taken together, PIG-U and the yeast orthologue Cdc91p are the fifth component of GPI transamidase that may be involved in the recognition of either the GPI attachment signal or the lipid portion of GPI.
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“The five subunits of S. cerevisiae GPI-T are Gpi8 (which contains the catalytic active site), Gpi16, Gaa1, Gpi17, and Gab1 (88,92,93).”
From Computed structures of core eukaryotic protein complexes (2021) · cites this paper as [88]
In the citing paper
“the hydrophobic Gab1 is also predicted to participate in anchor recognition (88).”
From Computed structures of core eukaryotic protein complexes (2021) · cites this paper as [88]
In the citing paper
“Glycosylphosphatidylinositol transamidase (GPI-T) is a pentameric enzyme complex of unknown structure (88–90)”
From Computed structures of core eukaryotic protein complexes (2021) · cites this paper as [88]
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Metadata
- Authors
- Hong, Y., Ohishi, K., Kang, J. Y., Tanaka, S., Inoue, N., Nishimura, J.-I., Maeda, Y., & Kinoshita, T.
- Affiliations
- Research Institute for Microbial Diseases, The University of Osaka (JP)
- ORCID
- Venue
- Molecular Biology of the Cell
- Published
- 2003-05
- Volume
- 14
- Issue
- 5
- Pages
- 1780-1789
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- PMC
- PMC165076
- OpenAlex
- W2158957854
- Cited by
- 121
- References
- 30
- Citations by year
- 2025: 3 · 2024: 6 · 2023: 1 · 2022: 4 · 2021: 12 · 2020: 11 · 2019: 9 · 2018: 6 · 2017: 6 · 2016: 1 · 2015: 2 · 2014: 5 · 2013: 5 · 2012: 5
- FWCI
- 2.92
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Molecular Biology of the Cell (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- glycosylphosphatidylinositol anchoring, GPI-anchored proteins, protein complex formation, CHO cells, Saccharomyces cerevisiae
- MeSH terms
- Acyltransferases, Amino Acid Sequence, Animals, Cricetinae, Humans, Molecular Sequence Data, Saccharomyces cerevisiae, Glycosylphosphatidylinositols, Protein Subunits
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