The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Post-Golgi Sec Proteins Are Required for Autophagy in Saccharomyces cerevisiae
Open accessAbstract
In eukaryotic cells, autophagy mediates the degradation of cytosolic contents in response to environmental change. Genetic analyses in fungi have identified over 30 autophagy-related (ATG) genes and provide substantial insight into the molecular mechanism of this process. However, one essential issue that has not been resolved is the origin of the lipids that form the autophagosome, the sequestering vesicle that is critical for autophagy. Here, we report that two post-Golgi proteins, Sec2 and Sec4, are required for autophagy. Sec4 is a Rab family GTPase, and Sec2 is its guanine nucleotide exchange factor. In sec2 and sec4 conditional mutant yeast, the anterograde movement of Atg9, a proposed membrane carrier, is impaired during starvation conditions. Similarly, in the sec2 mutant, Atg8 is inefficiently recruited to the phagophore assembly site, which is involved in autophagosome biogenesis, resulting in the generation of fewer autophagosomes. We propose that following autophagy induction the function of Sec2 and Sec4 are diverted to direct membrane flow to autophagosome formation.
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Cites
Formation Process of Autophagosome Is Traced with Apg8/Aut7p in Yeast
Kirisako, T., Baba, M., Ishihara, N., Miyazawa, K., Ohsumi, M., Yoshimori, T., Noda, T., & Ohsumi, Y. (1999) · The Journal of Cell Biology · doi:10.1083/jcb.147.2.435 · Cited by 881
Reggiori, F., Tucker, K. A., Stromhaug, P. E., & Klionsky, D. J. (2004) · Developmental Cell · doi:10.1016/s1534-5807(03)00402-7 · Cited by 476
Self-citationKlionsky, D. J., & Emr, S. D. (1989) · The EMBO Journal · doi:10.1002/j.1460-2075.1989.tb08348.x · Cited by 325
Self-citationAtg9 Cycles Between Mitochondria and the Pre-Autophagosomal Structure in Yeasts
Reggiori, F., Shintani, T., Chong, H., Nair, U., & Klionsky, D. J. (2005) · Autophagy · doi:10.4161/auto.1.2.1840 · Cited by 266
Self-citationFengsrud, M., Erichsen, E. S., Berg, T. O., Raiborg, C., & Seglen, P. O. (2000) · European Journal of Cell Biology · doi:10.1078/0171-9335-00125 · Cited by 123
Cited by
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).
Klionsky, D. J., Abdelmohsen, K., Abe, A., Abedin, M. J., Abeliovich, H., Acevedo Arozena, A., Adachi, H., Adams, C. M., Adams, P. D., Adeli, K., et al. (2016) · Autophagy · doi:10.1080/15548627.2015.1100356 · Cited by 5,894
Self-citationThe Role of Atg Proteins in Autophagosome Formation
Mizushima, N., Yoshimori, T., & Ohsumi, Y. (2011) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-092910-154005 · Cited by 2,978
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1
Klionsky, D. J., Abdel-Aziz, A. K., Abdelfatah, S., Abdellatif, M., Abdoli, A., Abel, S., Abeliovich, H., Abildgaard, M. H., Abudu, Y. P., Acevedo-Arozena, A., et al. (2021) · Autophagy · doi:10.1080/15548627.2020.1797280 · Cited by 2,897
Self-citationThe autophagosome: origins unknown, biogenesis complex
Lamb, C. A., Yoshimori, T., & Tooze, S. A. (2013) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3696 · Cited by 1,314
Regulation of autophagy by ROS: physiology and pathology
Scherz-Shouval, R., & Elazar, Z. (2011) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2010.07.007 · Cited by 1,263
Metadata
- Authors
- Geng, J., Nair, U., Yasumura-Yorimitsu, K., & Klionsky, D. J.
- Venue
- Molecular Biology of the Cell
- Published
- 2010-07
- Volume
- 21
- Issue
- 13
- Pages
- 2257-2269
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- OpenAlex
- W2110866976
- Cited by
- 179
- References
- 5
- FWCI
- 14.26
- Type
- journal article
- Language
- en
- Open access
- green
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- Geng, J., Nair, U., Yasumura-Yorimitsu, K., & Klionsky, D. J. (2010). Post-Golgi Sec Proteins Are Required for Autophagy in Saccharomyces cerevisiae. Molecular Biology of the Cell. https://doi.org/10.1091/mbc.e09-11-0969
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
