CoChat
Sign Up
Cited by 118

The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.

Protrudin serves as an adaptor molecule that connects KIF5 and its cargoes in vesicular transport during process formation

Matsuzaki, Fumiko, Shirane, Michiko, Matsumoto, Masaki, Nakayama, Keiichi I.·2011·Molecular Biology of the Cell
DOI 10.1091/mbc.e11-01-0068PMC PMC3226478
Open this paper in CoChat — free account

Abstract

Neurons are highly polarized cells with long neurites. Vesicular transport is required for neurite extension. We recently identified protrudin as a key regulator of vesicular transport during neurite extension. Expression of protrudin in nonneuronal cells thus induces formation of neurite-like membrane protrusions. We adopted a proteomics approach to identify proteins that associate with protrudin. Among the protrudin-associated proteins, including many with a function related to intracellular trafficking, we focused on KIF5, a motor protein that mediates anterograde vesicular transport in neurons. A coimmunoprecipitation assay confirmed that endogenous protrudin and KIF5 interact in mouse brain. Overexpression of KIF5 induced the formation of membrane protrusions in HeLa cells, reminiscent of the effect of protrudin overexpression. Forced expression of both protrudin and KIF5 promoted protrusion extension in a synergistic manner, whereas depletion of either protein attenuated protrusion formation. Protrudin facilitated the interaction of KIF5 with Rab11, VAP-A and -B, Surf4, and RTN3, suggesting that protrudin serves as an adaptor protein and that the protrudin–KIF5 complex contributes to the transport of these proteins in neurons. Given that mutation of protrudin or KIF5 is a cause of human hereditary spastic paraplegia, the protrudin–KIF5 axis appears to be integral to neuronal function.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1091/mbc.e11-01-0068

Cites

3 of 3 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 58

  1. Belden WJ, 2001, Science, 294, 1528, 10.1126/science.1065224 · DOI
  2. Blasius TL, 2007, J Cell Biol, 176, 11, 10.1083/jcb.200605099 · DOI
  3. Cai D, 2007, J Cell Biol, 176, 51, 10.1083/jcb.200605097 · DOI
  4. Calhoun BC, 1997, Biochem J, 325, 559, 10.1042/bj3250559 · DOI
  5. Chai A, 2008, Hum Mol Genet, 17, 266, 10.1093/hmg/ddm303 · DOI
  6. Chen HJ, 2010, J Biol Chem, 285, 40266, 10.1074/jbc.M110.161398 · DOI
  7. Cho KI, 2007, Traffic, 8, 1722, 10.1111/j.1600-0854.2007.00647.x · DOI
  8. Deluca GC, 2004, Neuropathol Appl Neurobiol, 30, 576, 10.1111/j.1365-2990.2004.00587.x · DOI
  9. Ferreira A, 1992, J Cell Biol, 117, 595, 10.1083/jcb.117.3.595 · DOI
  10. Foster LJ, 2000, Traffic, 1, 512, 10.1034/j.1600-0854.2000.010609.x · DOI
  11. Futerman AH, 1996, Trends Neurosci, 19, 144, 10.1016/S0166-2236(96)80025-7 · DOI
  12. Glater EE, 2006, J Cell Biol, 173, 545, 10.1083/jcb.200601067 · DOI
  13. Goizet C, 2009, Hum Mutat, 30, E376, 10.1002/humu.20920 · DOI
  14. Goldstein AY, 2008, Curr Opin Neurobiol, 18, 495, 10.1016/j.conb.2008.10.003 · DOI
  15. Goldstein LS, 2001, Proc Natl Acad Sci USA, 98, 6999, 10.1073/pnas.111145298 · DOI
  16. Gyoeva FK, 2004, Biochemistry, 43, 13525, 10.1021/bi049288l · DOI
  17. He W, 2004, Nat Med, 10, 959, 10.1038/nm1088 · DOI
  18. Hirokawa N, 2008, Physiol Rev, 88, 1089, 10.1152/physrev.00023.2007 · DOI
  19. Hu J, 2009, Cell, 138, 549, 10.1016/j.cell.2009.05.025 · DOI
  20. Hu X, 2007, EMBO J, 26, 2755, 10.1038/sj.emboj.7601707 · DOI
  21. Jiang MY, 1995, Biophys J, 68, 283S
  22. Kessler A, 2000, Diabetologia, 43, 1518, 10.1007/s001250051563 · DOI
  23. Kimura T, 2005, J Neurochem, 93, 1371, 10.1111/j.1471-4159.2005.03063.x · DOI
  24. Konishi Y, 2009, Nat Neurosci, 12, 559, 10.1038/nn.2314 · DOI
  25. Lapierre LA, 2000, Adv Drug Deliv Rev, 41, 255, 10.1016/S0169-409X(00)00045-4 · DOI
  26. Lapierre LA, 2007, Am J Physiol Gastrointest Liver Physiol, 292, G1249, 10.1152/ajpgi.00505.2006 · DOI
  27. Lapierre LA, 1999, J Cell Sci, 112, 3723, 10.1242/jcs.112.21.3723 · DOI
  28. Mannan AU, 2008, Am J Hum Genet, 83, 128, 10.1016/j.ajhg.2008.05.018 · DOI
  29. Mannan AU, 2006, Neurogenetics, 7, 93, 10.1007/s10048-006-0034-4 · DOI
  30. Mannan AU, 2006, Am J Hum Genet, 79, 351, 10.1086/504927 · DOI
  31. Martignoni M, 2008, Am J Hum Genet, 83, 127, 10.1016/j.ajhg.2008.05.014 · DOI
  32. Matsumoto M, 2009, Proteomics, 9, 3549, 10.1002/pmic.200900011 · DOI
  33. Murayama KS, 2006, Eur J Neurosci, 24, 1237, 10.1111/j.1460-9568.2006.05005.x · DOI
  34. Nakata T, 1995, J Cell Biol, 131, 1039, 10.1083/jcb.131.4.1039 · DOI
  35. Nishimura AL, 2005, Hum Genet, 118, 499, 10.1007/s00439-005-0031-y · DOI
  36. Nishimura AL, 2004, Am J Hum Genet, 75, 822, 10.1086/425287 · DOI · Paper page
  37. Niwa S, 2008, Nat Cell Biol, 10, 1269, 10.1038/ncb1785 · DOI
  38. Park SH, 2010, J Clin Invest, 120, 1097, 10.1172/JCI40979 · DOI
  39. Pennetta G, 2002, Neuron, 35, 291, 10.1016/S0896-6273(02)00769-9 · DOI
  40. Prior M, 2010, Neurosci Biobehav Rev, 34, 1201, 10.1016/j.neubiorev.2010.01.017 · DOI
  41. Reeves JE, 1995, Mol Membr Biol, 12, 201, 10.3109/09687689509027508 · DOI
  42. Reid E, 1997, J Med Genet, 34, 499, 10.1136/jmg.34.6.499 · DOI
  43. Reid E, 2002, Am J Hum Genet, 71, 1189, 10.1086/344210 · DOI
  44. Saita S, 2009, J Biol Chem, 284, 13766, 10.1074/jbc.M807938200 · DOI
  45. Salinas S, 2007, J Neurosci Res, 85, 2778, 10.1002/jnr.21238 · DOI
  46. Salinas S, 2008, Lancet Neurol, 7, 1127, 10.1016/S1474-4422(08)70258-8 · DOI · Paper page
  47. Setou M, 2002, Nature, 417, 83, 10.1038/nature743 · DOI
  48. Sheehan D, 1996, NeuroReport, 7, 1297, 10.1097/00001756-199605170-00016 · DOI
  49. Shirane M, 2006, Science, 314, 818, 10.1126/science.1134027 · DOI
  50. Skehel PA, 1995, Science, 269, 1580, 10.1126/science.7667638 · DOI
  51. Su Q, 2004, Nat Cell Biol, 6, 941, 10.1038/ncb1169 · DOI
  52. Tang BL, 2001, J Neurochem, 79, 923, 10.1046/j.1471-4159.2001.00674.x · DOI
  53. Uhlig M, 2005, Mol Cell Endocrinol, 235, 1, 10.1016/j.mce.2005.02.004 · DOI
  54. Ullrich O, 1993, J Biol Chem, 268, 18143, 10.1016/S0021-9258(17)46822-0 · DOI
  55. Wang Z, 2008, Cell, 135, 535, 10.1016/j.cell.2008.09.057 · DOI · Paper page
  56. Weir ML, 2001, Biochem Biophys Res Commun, 286, 616, 10.1006/bbrc.2001.5437 · DOI
  57. Yada M, 2004, EMBO J, 23, 2116, 10.1038/sj.emboj.7600217 · DOI
  58. Yang JT, 1989, Cell, 56, 879, 10.1016/0092-8674(89)90692-2 · DOI

Cited by

Sorted by citations

Metadata

Authors
Matsuzaki, F., Shirane, M., Matsumoto, M., & Nakayama, K. I.
Venue
Molecular Biology of the Cell
Publisher
American Society for Cell Biology (ASCB)
Published
2011-12
Volume
22
Issue
23
Pages
4602-4620
ISSN
  • 1059-1524
  • 1939-4586
DOI
10.1091/mbc.e11-01-0068
PMC
PMC3226478
Cited by
118
References
3
Language
en

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review