Sub1 associates with Spt5 and influences RNA polymerase II transcription elongation rate
Open accessAbstract
The transcriptional coactivator Sub1 has been implicated in several steps of mRNA metabolism in yeast, such as the activation of transcription, termination, and 3′-end formation. In addition, Sub1 globally regulates RNA polymerase II phosphorylation, and most recently it has been shown that it is a functional component of the preinitiation complex. Here we present evidence that Sub1 plays a significant role in transcription elongation by RNA polymerase II (RNAPII). We show that SUB1 genetically interacts with the gene encoding the elongation factor Spt5, that Sub1 influences Spt5 phosphorylation of the carboxy-terminal domain of RNAPII largest subunit by the kinase Bur1, and that both Sub1 and Spt5 copurify in the same complex, likely during early transcription elongation. Indeed, our data indicate that Sub1 influences Spt5–Rpb1 interaction. In addition, biochemical and molecular data show that Sub1 influences transcription elongation of constitutive and inducible genes and associates with coding regions in a transcription-dependent manner. Taken together, our results indicate that Sub1 associates with Spt5 and influences Spt5–Rpb1 complex levels and consequently transcription elongation rate.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Sub1 increases RNA Pol II velocity in budding yeast.
“Sub1, which is a functional component of the pre‐initiation complex playing a role in TSS selection is also involved in transcription elongation and has been shown to increase RNA Pol II velocity in budding yeast (García et al, 2012).”
From RNA polymerase II speed: a key player in controlling and adapting transcriptome composition (2021) · cites this paper as García et al, 2012
The Results (section “Sub1 influences RNAPII transcription elongation rate”; page unavailable) report an elongation-rate defect when SUB1 is deleted. The Discussion (section “Sub1 affects transcription elongation efficiency and elongation rate”; page unavailable) says polymerase without Sub1 may be slower than wild type, but offers an alternative explanation: it may fail to resume transcription after stalling. Thus the paper supports a positive role for Sub1 in elongation, but not the unqualified statement that it was shown to increase polymerase velocity. (LLM, conf=0.85)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
The citation lists haven’t been fetched yet
References 82 · Cited by 25
- Authors
- García, A., Collin, A., & Calvo, O.
- Affiliations
- Consejo Superior de Investigaciones Científicas (ES)
- Corresponding author
- Calvo, Olga
- Venue
- Molecular Biology of the Cell
- Published
- 2012-11
- Volume
- 23
- Issue
- 21
- Pages
- 4297-4312
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- PMC
- PMC3484106
- OpenAlex
- W2002123602
- Cited by
- 25
- References
- 82
- Citations by year
- 2024: 3 · 2023: 1 · 2022: 2 · 2021: 2 · 2020: 1 · 2019: 1 · 2017: 4 · 2016: 4 · 2015: 3 · 2014: 3 · 2013: 1
- FWCI
- 0.90
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- Molecular Biology of the Cell (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion, cc-by-nc-sa)
- Funders
- Fundación Ramón Areces
- Ministerio de Ciencia e Innovación
- Junta de Castilla y León
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- SUB1, Spt5, RNA polymerase II, transcription elongation, elongation factors, RPB1, transcriptional coactivator, Saccharomyces cerevisiae, genetic interactions, preinitiation complex
- MeSH terms
- Chromatin, Chromosomal Proteins, Non-Histone, DNA-Binding Proteins, Phosphorylation, Protein Binding, RNA Polymerase II, RNA Splicing, RNA, Messenger, Saccharomyces cerevisiae, Transcription Factors, Gene Expression Regulation, Fungal, Open Reading Frames, Gene Deletion, Saccharomyces cerevisiae Proteins, Transcriptional Elongation Factors, Transcription Elongation, Genetic
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
