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Cited by 87Cellular transport and secretionAutophagy in Disease and TherapyCalcium signaling and nucleotide metabolism

ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus

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Nakai, Waka, Kondo, Yumika, Saitoh, Akina, Naito, Tomoki, Nakayama, Kazuhisa, Shin, Hye-Won·2013·Molecular Biology of the Cell
DOI 10.1091/mbc.e13-04-0197PMC PMC3744953
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Abstract

Small GTPases of the ADP-ribosylation factor (ARF) family, except for ARF6, mainly localize to the Golgi apparatus, where they trigger formation of coated carrier vesicles. We recently showed that class I ARFs (ARF1 and ARF3) localize to recycling endosomes, as well as to the Golgi, and are redundantly required for recycling of endocytosed transferrin. On the other hand, the roles of class II ARFs (ARF4 and ARF5) are not yet fully understood, and the complementary or overlapping functions of class I and class II ARFs have been poorly characterized. In this study, we find that simultaneous depletion of ARF1 and ARF4 induces extensive tubulation of recycling endosomes. Moreover, the depletion of ARF1 and ARF4 inhibits retrograde transport of TGN38 and mannose-6-phosphate receptor from early/recycling endosomes to the trans-Golgi network (TGN) but does not affect the endocytic/recycling pathway of transferrin receptor or inhibit retrograde transport of CD4-furin from late endosomes to the TGN. These observations indicate that the ARF1+ARF4 and ARF1+ARF3 pairs are both required for integrity of recycling endosomes but are involved in distinct transport pathways: the former pair regulates retrograde transport from endosomes to the TGN, whereas the latter is required for the transferrin recycling pathway from endosomes to the plasma membrane.

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  • Recruitment of AP-1 to the TGN is mediated by Arf1 and Arf4 small G proteins.

    “The recruitment of AP-1 to the TGN is known to be mediated by Arf1 and Arf4 small G proteins (Stamnes and Rothman, 1993; Lee et al., 2008; Nakai et al., 2013)”

    From Distinct anterograde trafficking pathways of BACE1 and amyloid precursor protein from the TGN and the regulation of amyloid-β production (2020) · cites this paper as Nakai et al., 2013

    The Results report that depleting ARF1 and ARF4 reduces AP-1 at the TGN, suggesting that they participate in its recruitment. The Discussion calls their role a possibility, so the source does not establish the citing sentence’s stronger claim that recruitment is known to be mediated by them. Page numbers are not provided in our copy. (LLM, conf=0.88)

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Sorted by citations

Authors
Nakai, W., Kondo, Y., Saitoh, A., Naito, T., Nakayama, K., & Shin, H.-W.
Affiliations
Kyoto University (JP)
Corresponding author
Shin, Hye-Won
ORCID
Venue
Molecular Biology of the Cell
Publisher
American Society for Cell Biology (ASCB)
Published
2013-08-15
Volume
24
Issue
16
Pages
2570-2581
ISSN
  • 1059-1524 (ISSN-L)
  • 1939-4586
DOI
10.1091/mbc.e13-04-0197
PMC
PMC3744953
OpenAlex
W1971325965
Cited by
87
References
0
Citations by year
2026: 8 · 2025: 10 · 2024: 6 · 2023: 8 · 2022: 7 · 2021: 9 · 2020: 6 · 2019: 12 · 2018: 2 · 2017: 3 · 2016: 5 · 2015: 4 · 2014: 6 · 2013: 1
FWCI
2.46
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • Molecular Biology of the Cell (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
  • Institutional Repositories DataBase (IRDB) (submittedVersion)
  • Kyoto University Research Information Repository (Kyoto University) (submittedVersion, cc-by-nc-sa)
Funders
  • Takeda Science Foundation
  • Inamori Foundation
  • Japan Society for the Promotion of Science (23570162)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
Keywords
Arf1, ARF4, ADP-ribosylation factor, recycling endosomes, retrograde transport, trans-Golgi network, Golgi apparatus, TGN38, mannose 6-phosphate receptor, transferrin recycling, transferrin receptor, late endosomes, coated vesicles, small GTPases, ARF3
MeSH terms
rab11 GTP-Binding Proteins, Golgi Apparatus, HeLa Cells, Humans, Membrane Glycoproteins, Receptors, Transferrin, Endosomes, CD4 Antigens, Receptor, IGF Type 2, rab GTP-Binding Proteins, rab4 GTP-Binding Proteins, ADP-Ribosylation Factors, ADP-Ribosylation Factor 1, Protein Transport, trans-Golgi Network, RNA Interference, RNA, Small Interfering, Furin, Cell Line, Tumor

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