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Cited by 81Cellular Mechanics and InteractionsProtein Kinase Regulation and GTPase SignalingMicrotubule and mitosis dynamics

Arp2/3 complex inhibition radically alters lamellipodial actin architecture, suspended cell shape, and the cell spreading process

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Henson, John H., Yeterian, Mesrob, Weeks, Richard M., Medrano, Angela E., Brown, Briana L., Geist, Heather L., Pais, Mollyann D., Oldenbourg, Rudolf +1 more·2015·Molecular Biology of the Cell
DOI 10.1091/mbc.e14-07-1244PMC PMC4342025
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Abstract

The consequences of drug-based Arp2/3 inhibition in sea urchin coelomocytes include dramatic changes in lamellipodial architecture, a lamellipodial-to-filopodial shape change in suspended cells, and a novel actin structural organization in spreading cells. The generation of actin arcs induced by Arp2/3 inhibition is arrested by formin inhibition.

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Authors
Henson, J. H., Yeterian, M., Weeks, R. M., Medrano, A. E., Brown, B. L., Geist, H. L., Pais, M. D., Oldenbourg, R., & Shuster, C. B.
Affiliations
  • Dickinson College (US)
  • Marine Biological Laboratory (US)
  • New Mexico State University (US)
Corresponding author
Henson, John H.
ORCID
Venue
Molecular Biology of the Cell
Publisher
American Society for Cell Biology (ASCB)
Published
2015-03
Volume
26
Issue
5
Pages
887-900
ISSN
  • 1059-1524 (ISSN-L)
  • 1939-4586
DOI
10.1091/mbc.e14-07-1244
PMC
PMC4342025
OpenAlex
W2093304039
Cited by
81
References
0
Citations by year
2026: 6 · 2025: 7 · 2024: 17 · 2023: 9 · 2022: 4 · 2021: 10 · 2020: 4 · 2019: 11 · 2018: 5 · 2017: 3 · 2016: 4 · 2015: 1
FWCI
2.08
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • Molecular Biology of the Cell (publishedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
  • Open Access Server of the Woods Hole Scientific Community (Woods Hole Scientific Community) (submittedVersion, cc-by-nc-sa)
Funders
  • National Science Foundation (0856704, 1412688, 1412734)
  • University of Pennsylvania
  • University of Pittsburgh
  • National Institutes of Health
  • National Institute of Biomedical Imaging and Bioengineering (R01EB002583, EB002583)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Keywords
Arp2/3 complex, lamellipodia, actin cytoskeleton, cell spreading, coelomocytes, sea urchins, keratocytes, Drosophila S2 cells, actin filament nucleation
MeSH terms
Animals, Drosophila, Goldfish, Indoles, Actin Cytoskeleton, Microscopy, Electron, Pseudopodia, Strongylocentrotus, Cell Shape, Actin-Related Protein 2-3 Complex

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