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Cited by 27Cellular transport and secretionNerve injury and regenerationNeuroscience and Neuropharmacology Research

Retrolinkin recruits the WAVE1 protein complex to facilitate BDNF-induced TrkB endocytosis and dendrite outgrowth

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Xu, Chenchang, Fu, Xiuping, Zhu, Shaoxia, Liu, Jia-Jia·2016·Molecular Biology of the Cell
DOI 10.1091/mbc.e16-05-0326PMC PMC5170866
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Abstract

Retrolinkin, a neuronal membrane protein, coordinates with endophilin A1 and mediates early endocytic trafficking and signal transduction of the ligand–receptor complex formed between brain-derived neurotrophic factor (BDNF) and its receptor, tropomyosin-related kinase B (TrkB), in dendrites of CNS neurons. Here we report that retrolinkin interacts with the CYFIP1/2 subunit of the WAVE1 complex, a member of the WASP/WAVE family of nucleation-promoting factors that binds and activates the Arp2/3 complex to promote branched actin polymerization. WAVE1, not N-WASP, is required for BDNF-induced TrkB endocytosis and dendrite outgrowth. Disruption of the interaction between retrolinkin and CYFIP1/2 impairs recruitment of WAVE1 to neuronal plasma membrane upon BDNF addition and blocks internalization of activated TrkB. We also show that WAVE1-mediated endocytosis of BDNF-activated TrkB is actin dependent and clathrin independent. These results not only reveal the mechanistic role of retrolinkin in BDNF–TrkB endocytosis, but also indicate that WASP/WAVE-dependent actin polymerization during endocytosis is regulated by cell type–specific and cargo-specific modulators.

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  • Retrolinkin interacts with CYFIP2 in the WAVE1 complex and facilitates BDNF-induced TRKB endocytosis and axonal outgrowth.

    “Retrolinkin interacts with CYFIP2 in the WAVE1 complex and facilitates BDNF-induced TRKB endocytosis and axonal outgrowth58.”

    From The WAVE complex in developmental and adulthood brain disorders (2025) · cites this paper as [58]

    The Discussion reports that retrolinkin binds CYFIP1/2 in the WAVE1 complex and facilitates BDNF-induced TrkB endocytosis. It reports BDNF-induced dendrite outgrowth, not axonal outgrowth; that part of the claim is unsupported. (LLM, conf=0.90)

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Cited by

Sorted by citations

Authors
Xu, C., Fu, X., Zhu, S., & Liu, J.-J.
Affiliations
Chinese Academy of Sciences (CN)
Corresponding author
Liu, Jia-Jia
ORCID
Venue
Molecular Biology of the Cell
Publisher
American Society for Cell Biology (ASCB)
Published
2016-11
Volume
27
Issue
21
Pages
3342-3356
ISSN
  • 1059-1524 (ISSN-L)
  • 1939-4586
DOI
10.1091/mbc.e16-05-0326
PMC
PMC5170866
OpenAlex
W2515149348
Cited by
27
References
1
Citations by year
2026: 1 · 2025: 2 · 2024: 3 · 2023: 4 · 2022: 6 · 2021: 3 · 2020: 1 · 2019: 3 · 2018: 2 · 2017: 2
FWCI
1.60
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • Molecular Biology of the Cell (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China
  • Ministry of Science and Technology of the People's Republic of China
  • University of Science and Technology of China
  • Institute of Biophysics, Chinese Academy of Sciences
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
WAVE1, BDNF signaling, dendritic outgrowth, Arp2/3 complex, actin polymerization, clathrin-independent endocytosis, endophilin A1
MeSH terms
Actins, Animals, Clathrin, Dendrites, Endocytosis, Membrane Proteins, Nerve Tissue Proteins, Neurons, Signal Transduction, Brain-Derived Neurotrophic Factor, Receptor, trkB, Protein Transport, Vesicular Transport Proteins, Adaptor Proteins, Signal Transducing, Wiskott-Aldrich Syndrome Protein Family, Actin-Related Protein 2-3 Complex, Mice

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