Actin assembly produces sufficient forces for endocytosis in yeast
Open accessAbstract
We formulated a spatially resolved model to estimate forces exerted by a polymerizing actin meshwork on an invagination of the plasma membrane during endocytosis in yeast cells. The model, which approximates the actin meshwork as a visco-active gel exerting forces on a rigid spherocylinder representing the endocytic invagination, is tightly constrained by experimental data. Simulations of the model produce forces that can overcome resistance of turgor pressure in yeast cells. Strong forces emerge due to the high density of polymerized actin in the vicinity of the invagination and because of entanglement of the meshwork due to its dendritic structure and cross-linking. The model predicts forces orthogonal to the invagination that are consistent with formation of a flask shape, which would diminish the net force due to turgor pressure. Simulations of the model with either two rings of nucleation-promoting factors (NPFs) as in fission yeast or a single ring of NPFs as in budding yeast produce enough force to elongate the invagination against the turgor pressure.
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In yeast, active force generation by actin polymerization has been long established as the mechanism by which actin supports endocytosis.
“In yeast, active force generation by actin polymerization has been long established as the mechanism by which actin supports endocytosis85,86.”
From Membrane compression by synaptic vesicle exocytosis triggers ultrafast endocytosis (2023) · cites this paper as [85]
The Introduction says actin assembly is necessary for membrane invagination in yeast and that polymerizing actin was believed to produce the required forces. The Discussion says it had remained unclear whether actin assembly generated enough force to overcome turgor pressure. Thus the force-generating mechanism is supported, but describing it as long established overstates how settled the paper says it was. (LLM, conf=0.84)
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Membrane compression by synaptic vesicle exocytosis triggers ultrafast endocytosis
Ogunmowo, T. H., Jing, H., Raychaudhuri, S., Kusick, G. F., Imoto, Y., Li, S., Itoh, K., Ma, Y., Jafri, H., Dalva, M. B., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-38595-2 · Cited by 51 · see citing works
“In yeast, active force generation by actin polymerization has been long established as the mechanism by which actin supports endocytosis 85,86 .”
- Authors
- Nickaeen, M., Berro, J., Pollard, T. D., & Slepchenko, B. M.
- Affiliations
- UConn Health (US)
- Yale University (US)
- Corresponding author
- Slepchenko, Boris M.
- Venue
- Molecular Biology of the Cell
- Published
- 2019-07-22
- Volume
- 30
- Issue
- 16
- Pages
- 2014-2024
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- PMC
- PMC6727779
- OpenAlex
- W2955379069
- Cited by
- 27
- References
- 0
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 1 · 2023: 5 · 2022: 6 · 2021: 4 · 2020: 3 · 2019: 5
- FWCI
- 2.38
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- Molecular Biology of the Cell (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Funders
- National Science Foundation
- University of Connecticut
- National Institutes of Health
- National Institute of General Medical Sciences (R01GM026338, R01GM115636, P41GM103313)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Keywords
- actin polymerization, endocytosis, yeast, membrane invagination, turgor pressure, viscoelastic gels, actin cross-linking, nucleation-promoting factors, Schizosaccharomyces pombe, Saccharomyces cerevisiae
- MeSH terms
- Actins, Biomechanical Phenomena, Cell Membrane, Computer Simulation, Endocytosis, Kinetics, Actin Cytoskeleton, Models, Biological, Saccharomyces cerevisiae, Viscosity
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