CoChat
Sign Up
Cited by 20Lipid metabolism and biosynthesisLipid metabolism and disordersCellular transport and secretion

PI(3)P and DFCP1 regulate the biogenesis of lipid droplets

Open access
Lukmantara, Ivan, Chen, Fang, Mak, Hoi Yin, Zadoorian, Armella, Du, Ximing, Xiao, Fanqian Nicole, Norris, Dougall MacMurray, Pandzic, Elvis +3 more·2022·Molecular Biology of the Cell
DOI 10.1091/mbc.e22-07-0279PMC PMC9727793
Open this paper in CoChat — free account

Abstract

PI(3)P and DFCP1, both known for their association with autophagy initiation, regulate the biogenesis of lipid droplets.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1091/mbc.e22-07-0279

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 50

  1. Arlt H Sui X Folger B Adams C Chen X Remme R Hamprecht FA DiMaio F Liao M Goodman JM, et al. (2022). Seipin forms a flexible cage at lipid droplet formation sites. Nat Struct Mol Biol 29, 194– 202. 35210614 PMC8930772
  2. Axe EL Walker SA Manifava M Chandra P Roderick HL Habermann A Griffiths G Ktistakis NT (2008). Autophagosome formation from membrane compartments enriched in phosphatidylinositol 3-phosphate and dynamically connected to the endoplasmic reticulum. J Cell Biol 182, 685– 701. 18725538 PMC2518708
  3. Benedetti L Marvin JS Falahati H Guillen-Samander A Looger LL De Camilli P (2020). Optimized vivid-derived magnets photodimerizers for subcellular optogenetics in mammalian cells. eLife 9, e63230. 33174843 PMC7735757
  4. Bernales S McDonald KL Walter P (2006). Autophagy counterbalances endoplasmic reticulum expansion during the unfolded protein response. PLoS Biol 4, e423. 17132049 PMC1661684
  5. Bosch M Sanchez-Alvarez M Fajardo A Kapetanovic R Steiner B Dutra F Moreira L Lopez JA Campo R Mari M, et al. (2020). Mammalian lipid droplets are innate immune hubs integrating cell metabolism and host defense. Science 370, eaay8085. 33060333
  6. Chung J Wu X Lambert TJ Lai ZW Walther TC Farese RV Jr (2019). LDAF1 and seipin form a lipid droplet assembly complex. Dev Cell 51, 551–563.e557. 31708432 PMC7235935
  7. Cui X Wang Y Tang Y Liu Y Zhao L Deng J Xu G Peng X Ju S Liu G Yang H (2011). Seipin ablation in mice results in severe generalized lipodystrophy. Hum Mol Genet 20, 3022– 3030. 21551454
  8. Du X Yang H (2021). Seipin regulates the formation of nuclear lipid droplets from a distance. J Cell Biol 220, e202011166. 33320190 PMC7745754
  9. Fei W Du X Yang H (2011a). Seipin, adipogenesis and lipid droplets. Trends Endocrinol Metab 22, 204– 210. 21497513
  10. Fei W Shui G Gaeta B Du X Kuerschner L Li P Brown AJ Wenk MR Parton RG Yang H (2008). Fld1p, a functional homologue of human seipin, regulates the size of lipid droplets in yeast. J Cell Biol 180, 473– 482. 18250201 PMC2234226
  11. Fei W Shui G Zhang Y Krahmer N Ferguson C Kapterian TS Lin RC Dawes IW Brown AJ Li P, et al. (2011b). A role for phosphatidic acid in the formation of “supersized” lipid droplets. PLoS Genet 7, e1002201. 21829381 PMC3145623
  12. Fei W Wang H Fu X Bielby C Yang H (2009). Conditions of endoplasmic reticulum stress stimulate lipid droplet formation in Saccharomyces cerevisiae. Biochem J 424, 61– 67. 19708857
  13. Gao G Sheng Y Yang H Chua BT Xu L (2019a). DFCP1 associates with lipid droplets. Cell Biol Int 43, 1492– 1504. 31293035
  14. Gao M Huang X Song BL Yang H (2019b). The biogenesis of lipid droplets: lipids take center stage. Prog Lipid Res 75, 100989. 31351098
  15. Gao M Liu L Wang X Mak HY Liu G Yang H (2020). GPAT3 deficiency alleviates insulin resistance and hepatic steatosis in a mouse model of severe congenital generalized lipodystrophy. Hum Mol Genet 29, 432– 443. 31873720
  16. Gillooly DJ Morrow IC Lindsay M Gould R Bryant NJ Gaullier JM Parton RG Stenmark H. (2000) Localization of phosphatidylinositol 3-phosphate in yeast and mammalian cells. EMBO J 19, 4577– 4588. 10970851 PMC302054
  17. Grippa A Buxo L Mora G Funaya C Idrissi FZ Mancuso F Gomez R Muntanya J Sabido E Carvalho P (2015). The seipin complex Fld1/Ldb16 stabilizes ER-lipid droplet contact sites. J Cell Biol 211, 829– 844. 26572621 PMC4657162
  18. Han S Binns DD Chang YF Goodman JM (2015). Dissecting seipin function: the localized accumulation of phosphatidic acid at ER/LD junctions in the absence of seipin is suppressed by Sei1p(DeltaNterm) only in combination with Ldb16p. BMC Cell Biol 16, 29. 26637296 PMC4670494
  19. Hurley JH Young LN (2017). Mechanisms of autophagy initiation. Annu Rev Biochem 86, 225– 244. 28301741 PMC5604869
  20. Jiang M Gao M Wu C He H Guo X Zhou Z Yang H Xiao X Liu G Sha J (2014). Lack of testicular seipin causes teratozoospermia syndrome in men. Proc Natl Acad Sci USA 111, 7054– 7059. 24778225 PMC4024893
  21. Joshi AS Zhang H Prinz WA (2017). Organelle biogenesis in the endoplasmic reticulum. Nat Cell Biol 19, 876– 882. 28714972
  22. Kimura S Noda T Yoshimori T (2007). Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3. Autophagy 3, 452– 460. 17534139
  23. Klug YA Deme JC Corey RA Renne MF Stansfeld PJ Lea SM Carvalho P (2021). Mechanism of lipid droplet formation by the yeast Sei1/Ldb16 Seipin complex. Nat Commun 12, 5892. 34625558 PMC8501077
  24. Li D Zhao YG Li D Zhao H Huang J Miao G Feng D Liu P Li D Zhang H (2019). The ER-localized protein DFCP1 modulates ER-lipid droplet contact formation. Cell Rep 27, 343– 358.e345. 30970241
  25. Lundin C Nordstrom R Wagner K Windpassinger C Andersson H von Heijne G Nilsson I (2006). Membrane topology of the human seipin protein. FEBS Lett 580, 2281– 2284. 16574104
  26. Madeira JB Masuda CA Maya-Monteiro CM Matos GS Montero-Lomeli M Bozaquel-Morais BL (2015). TORC1 inhibition induces lipid droplet replenishment in yeast. Mol Cell Biol 35, 737– 746. 25512609 PMC4301715
  27. Magre J Delepine M Khallouf E Gedde-Dahl T Jr Van Maldergem L Sobel E Papp J Meier M Megarbane A Bachy A, et al. (2001). Identification of the gene altered in Berardinelli-Seip congenital lipodystrophy on chromosome 11q13. Nat Genet 28, 365– 370. 11479539
  28. Matsunaga K Morita E Saitoh T Akira S Ktistakis NT Izumi T Noda T Yoshimori T (2010). Autophagy requires endoplasmic reticulum targeting of the PI3-kinase complex via Atg14L. J Cell Biol 190, 511– 521. 20713597 PMC2928018
  29. Melia TJ Lystad AH Simonsen A (2020). Autophagosome biogenesis: from membrane growth to closure. J Cell Biol 219, e202002085. 32357219 PMC7265318
  30. Mercer TJ Gubas A Tooze SA (2018). A molecular perspective of mammalian autophagosome biogenesis. J Biol Chem 293, 5386– 5395. 29371398 PMC5900756
  31. Mizushima N Yoshimori T Ohsumi Y (2011). The role of Atg proteins in autophagosome formation. Annu Rev Cell Dev Biol 27, 107– 132. 21801009
  32. Nguyen TB Louie SM Daniele JR Tran Q Dillin A Zoncu R Nomura DK Olzmann JA (2017). DGAT1-dependent lipid droplet biogenesis protects mitochondrial function during starvation-induced autophagy. Dev Cell 42, 9– 21.e25. 28697336 PMC5553613
  33. Ogata M Hino S Saito A Morikawa K Kondo S Kanemoto S Murakami T Taniguchi M Tanii I Yoshinaga K, et al. (2006). Autophagy is activated for cell survival after endoplasmic reticulum stress. Mol Cell Biol 26, 9220– 9231. 17030611 PMC1698520
  34. Olzmann JA Carvalho P (2019). Dynamics and functions of lipid droplets. Nat Rev Mol Cell Biol 20, 137– 155. 30523332 PMC6746329
  35. Pagac M Cooper DE Qi Y Lukmantara IE Mak HY Wu Z Tian Y Liu Z Lei M Du X, et al. (2016). SEIPIN regulates lipid droplet expansion and adipocyte development by modulating the activity of glycerol-3-phosphate acyltransferase. Cell Rep 17, 1546– 1559. 27806294 PMC5647143
  36. Rambold AS Cohen S Lippincott-Schwartz J (2015). Fatty acid trafficking in starved cells: regulation by lipid droplet lipolysis, autophagy, mitochondrial fusion dynamics. Dev Cell 32, 678– 692. 25752962 PMC4375018
  37. Salo VT Belevich I Li S Karhinen L Vihinen H Vigouroux C Magre J Thiele C Holtta-Vuori M Jokitalo E Ikonen E (2016). Seipin regulates ER-lipid droplet contacts and cargo delivery. EMBO J 35, 2699– 2716. 27879284 PMC5167346
  38. Salo VT Li S Vihinen H Holtta-Vuori M Szkalisity A Horvath P Belevich I Peranen J Thiele C Somerharju P, et al. (2019). Seipin facilitates triglyceride flow to lipid droplet and counteracts droplet ripening via endoplasmic reticulum contact. Dev Cell 50, 478– 493.e479. 31178403
  39. Santinho A Salo VT Chorlay A Li S Zhou X Omrane M Ikonen E Thiam AR (2020). Membrane curvature catalyzes lipid droplet assembly. Curr Biol 30, 2481– 2494. 32442467
  40. Sim MF Dennis RJ Aubry EM Ramanathan N Sembongi H Saudek V Ito D O’Rahilly S Siniossoglou S Rochford JJ (2012). The human lipodystrophy protein seipin is an ER membrane adaptor for the adipogenic PA phosphatase lipin 1. Mol Metabol 2, 38– 46. PMC3757660 24024128
  41. Soltysik K Ohsaki Y Tatematsu T Cheng J Maeda A Morita SY Fujimoto T (2021). Nuclear lipid droplets form in the inner nuclear membrane in a seipin-independent manner. J Cell Biol 220, e202005026. 33315072 PMC7737703
  42. Sui X Arlt H Brock KP Lai ZW DiMaio F Marks DS Liao M Farese RV Jr Walther TC (2018). Cryo-electron microscopy structure of the lipid droplet-formation protein seipin. J Cell Biol 217, 4080– 4091. 30327422 PMC6279392
  43. Szymanski KM Binns D Bartz R Grishin NV Li WP Agarwal AK Garg A Anderson RG Goodman JM (2007). The lipodystrophy protein seipin is found at endoplasmic reticulum lipid droplet junctions and is important for droplet morphology. Proc Natl Acad Sci USA 104, 20890– 20895. 18093937 PMC2409237
  44. Taylor FR Parks LW (1979). Triaglycerol metabolism in Saccharomyces cerevisiae. Relation to phospholipid synthesis. Biochim Biophys Acta 575, 204– 214. 389291
  45. Walther TC Chung J Farese RV Jr (2017). Lipid droplet biogenesis. Annu Rev Cell Dev Biol 33, 491– 510. 28793795 PMC6986389
  46. Wang H Becuwe M Housden BE Chitraju C Porras AJ Graham MM Liu XN Thiam AR Savage DB Agarwal AK, et al. (2016). Seipin is required for converting nascent to mature lipid droplets. eLife 5, e16582. 27564575 PMC5035145
  47. Wolinski H Hofbauer HF Hellauer K Cristobal-Sarramian A Kolb D Radulovic M Knittelfelder OL Rechberger GN Kohlwein SD (2015). Seipin is involved in the regulation of phosphatidic acid metabolism at a subdomain of the nuclear envelope in yeast. Biochim Biophys Acta 1851, 1450– 1464. 26275961
  48. Yan R Qian H Lukmantara I Gao M Du X Yan N Yang H (2018). Human SEIPIN binds anionic phospholipids. Dev Cell 47, 248– 256.e244. 30293840
  49. Yang H Bard M Bruner DA Gleeson A Deckelbaum RJ Aljinovic G Pohl TM Rothstein R Sturley SL (1996). Sterol esterification in yeast: a two-gene process. Science 272, 1353– 1356. 8650549
  50. Zhang Q Chieu HK Low CP Zhang S Heng CK Yang H (2003). Schizosaccharomyces pombe cells deficient in triacylglycerols synthesis undergo apoptosis upon entry into the stationary phase. J Biol Chem 278, 47145– 47155. 12963726

Cited by

Sorted by citations

Authors
Lukmantara, I., Chen, F., Mak, H. Y., Zadoorian, A., Du, X., Xiao, F. N., Norris, D. M., Pandzic, E., Whan, R., Zhong, Q., & Yang, H.
Affiliations
  • UNSW Sydney (AU)
  • Shanghai Jiao Tong University (CN)
  • Katharina Gaus Light Microscopy Facility, Mark Wainwright Analytical Center, University of New South Wales, Sydney, NSW 2052, Australia
Corresponding author
Yang, Hongyuan
ORCID
Venue
Molecular Biology of the Cell
Publisher
American Society for Cell Biology (ASCB)
Published
2022-12-01
Volume
33
Issue
14
Pages
ar131-ar131
ISSN
  • 1059-1524 (ISSN-L)
  • 1939-4586
DOI
10.1091/mbc.e22-07-0279
PMC
PMC9727793
OpenAlex
W4296701608
Cited by
20
References
1
Citations by year
2026: 5 · 2025: 4 · 2024: 6 · 2023: 5
FWCI
1.58
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • Molecular Biology of the Cell (publishedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
Keywords
lipid droplet formation, phosphatidylinositol 3-phosphate, seipin, anionic phospholipids, fat deposition
MeSH terms
Phospholipids, Phosphatidylinositol Phosphates, Lipid Metabolism, Lipid Droplets

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review