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A Sensitive Fluorimetric Assay for Serum Angiotensin-con venrting Enzyme
Abstract
A simple, rapid, highly sensitive and reproducible assay for angiotensin-converting enzyme in untreated serum is described. It is based on the conversion of the substrate analog, hippuryl-L-histidyl-L-leucine (5 mM in 0.1 M K phosphate, pH 8.3-0.3 M NaCl) to hippurate and L-histidyl-L-leucine, which is quantified spectrofluorimetrically (lamdba excitation = 360 nm; lamdba fluorescence = 500 nm) by formation of a fluorescent adduct with ophthaldialdehyde. The chloride requirement and inhibition and activation patterns correspond to those for angiotensin-converting enzyme. The Km for hippuryl-L-histidyl-L-leucine was 1.33 mM. The mean value of serum angiotensin-converting enzyme for 58 normal human subjects (mean age, 32 years; range 19-57) was 32.2 +/- 1.30 (SE), with a standard deviation of 9.87 nmol/min/ml serum. The assay is useful for the diagnosis and possible management of sarcoidosis and may have other applications in the future.
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Cited by
ACE and ACE2 Activity in Diabetic Mice
Wysocki, J., Ye, M., Soler, M. J., Gurley, S. B., Xiao, H. D., Bernstein, K. E., Coffman, T. M., Chen, S., & Batlle, D. (2006) · Diabetes · doi:10.2337/db06-0033 · Cited by 339
Moholisa, R. R., Rayner, B. R., Patricia Owen, E., Schwager, S. L. U., Stark, J. S., Badri, M., Cupido, C. L., & Sturrock, E. D. (2013) · The Journal of Clinical Hypertension · doi:10.1111/jch.12104 · Cited by 42
Metadata
- Authors
- Friedland, J., & Silverstein, E.
- Venue
- American Journal of Clinical Pathology
- Publisher
- Oxford University Press (OUP)
- Published
- 1976-08-01
- Volume
- 66
- Issue
- 2
- Pages
- 416-424
- ISSN
- 0002-9173 (ISSN-L)
- 1943-7722
- OpenAlex
- W1887255777
- Cited by
- 456
- References
- 0
- FWCI
- 9.06
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: A Sensitive Fluorimetric Assay for Serum Angiotensin-con venrting Enzyme. https://app.cochat.ai/papers/10.1093/ajcp/66.2.416
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- Friedland, J., & Silverstein, E. (1976). A Sensitive Fluorimetric Assay for Serum Angiotensin-con venrting Enzyme. American Journal of Clinical Pathology. https://doi.org/10.1093/ajcp/66.2.416
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